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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2003; 49(6): 31-37


Effects of inhibiting protein kinase C on Ca[sup]2+[/sup]-sensitivityof contractile machinery in vascular smooth muscle under vasospasm of different genesis

A.A. Pavlova, V.F. Sagach, A.I. Soloviev

    Institute of Pharmacology and Toxicology Academy ofMedical Sciences of Ukraine, Kiev;А.А. Bogomoletz Institute of Physiology NationalAcademy of Sciences of Ukraine, Kiev


Abstract

The aim of the study was to investigate the role of protein kinase C (PKC) in changes in myofilament Ca2+-sensitivity of vascular smooth muscle cells (SMC) in rats at different vasospastic states: hypoxic pulmonary vasoconstriction, genetically determined hypertension, and hypertension resulted from ionizing radiation. All vasospastic states demonstrated rightward shifts in pCa-tension curves suggesting that myofilament Ca2+-sensitivity had increased. In chemically (?-es- cin) skinned pulmonary artery, hypoxia-induced increase in myofilament Ca2+-sensitivity was completely abolished by PKC inhibitor chelerythrine. The similar results were demon- strated in skinned aorta SMC of spontaneously hypertensive rats where an increase in myofilament Ca2+-sensitivity was also abolished by PKC inhibitors chelerythrine and stauro- sporine. The chelerythrine partially inhibited myofilament Ca2+-sensitivity that had increased following ?-radiation. The data suggest the key role of PKC activity in modulation of myofilament Ca2+-sensitivity in SMC. We conclude that PKC- mediated increase in myofilament Ca2+-sensitivity is one of the main mechanisms which contribute to the vasospasm of different genesis.

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