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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2003; 49(5): 105-111


Myelination and demyelinationprocesses in the rat cerebellum cellculture: an electron microscopic study.

T.A. Pivneva, E.V. Kolotushkina, G.G. Skibo

    A.A.Bogomoletz Institute of Physiology National Academy ofSciences of Ukraine, Kiev


Abstract

We observed manifestations of the myelination process in dissociated culture of the cerebellar tissue of newborn rats and modifications of the structure of myelin sheaths after treating the culture with a demyelinating factor, blood serum of patients suffering from multiple sclerosis (MS). On day in vitro (DIV) 26, in the control myelin sheaths of the axons demonstrated the closest resemblance to those observed in vivo, and we selected this term for inducing demyelination. Addition of the serum of MS patients to the culturing medium evoked rapid (in 3-6 hours) dramatic changes in the ultrastructure of myelin sheaths; these were a decrease in the number of the lamellae, their splitting and invagination, formation of vesicles, etc. The serum of MS patients in an acute stage of the disease exerted more intensive demyelination effects than that of patients in a remission stage.

References

  1. Avtsyn A.P., Shakhlamov V.A. Ultrastructural principles of general pathology of the cell. – M.: Meditsina, 1979. – P. 85 – 106.
  2. Bernard C.C.C., de Rosbo N.K. Multiple sclerosis: an autoimmune disease of multifactorial Etiology // Curr. Opin. Immunol. – 1992. – 4, N 2. – P. 760 – 765.
  3. Butt A.M., Ibrahim M., Gregson N., Berry M. Differential expression of myelin-associated glycoprotein (MAG) in oligodendrocyte unit phenotypes in the adult rat anterior medullary velum // J. Neurocytol. – 1998. – 27, N 4. – P. 271 – 280.
  4. D’Souza S.D., Bonetti B., Balasingam V., Cashman N.R., Barker P.A., Troutt A.B., Raine C.S., Antel J.P. Multiple sclerosis: Fas signalling in oligodendrocyte cell death // J. Exp. Med. – 1996. – 184, N 6. – P. 2361 – 2370.
  5. Fedoroff S., Richardson A. Protocols for neural tissue culture. – Totowa, New Jersey, Human Press, 1997.
  6. Feldman M., Peters A. Ballooning of myelin sheaths in normally aged macaques // J. Neurocytol. – 1998. – 27, N 8. – P. 605 – 614.
  7. Johns T.C., Bernard C.C.A. The structure of myelin oligodendrocyte glycoprotein // J. Neurochem. – 1999. – 72, N 1. – P. 1 – 9.
  8. Khokhlov A.P., Savchenko Yu.N. Myelin and molecular basis of myelination process // Korsakov Neurol. Psychiatry J. – 1990. – 90, N 12, – P. 104 – 109.
  9. 9. Moonen G., Neale E.A., Macdonald R.L. et al. Cerebellar macroneurons in microexplant cell culture: methodology, basic electrophysiology and morphology after horseradish peroxidase injection // Dev. Brain Res. – 1982. – 281, N 1. – P. 59 – 73.
  10. 10. Nakahara J., Tan-Takeuchi K., Seiwa C. et al. Myelin basic protein is necessary for the regulation of myelinassociated glycoprotein expression in mouse oligodengroglia // Neurosci. Lett. – 2001. – 298, N 3. – P. 163 – 166.
  11. Notterpek L.M., Bullock P.N., Malek-Hedayat S., Rome L.H. Myelination in cerebellar slice culture: development of a system amenable to biochemical analysis // J. Neurosci. Res. – 1993. – 36, N 6. – P. 621 – 634.
  12. Pivneva T.A., Kolotushkina E.V., Melnik N.A. Mechanisms of demyelination process and its modelling // Neurohysiol. – 1999. – 31, N 6. – P. 497 – 509.
  13. Roitbak A.I. Glia and its role in the nerve activity. – S-Pb: Nauka, 1993.
  14. Yin X., Crawford T.O., Griffin J.W. et al. Myelin-associated glycoprotein is myelin signal that modulate the caliber of myelinated axons // J. Neurosci. – 1998. – 18, N 6. – P. 1953 – 1962.
  15. Zaprianova E.T., Deleva D.D. Localization of synthesis and transport of phospholipids at myelination in the central nerve system // Morphology. – 1998. – 113, N 1. – P. 43 – 46.
  16. Zavalishin I.A., Zakharova M.N., Askarova L.Sh. et al. Current directions in studying of demyelinating diseases pathogenesis // Korsakov Neurol. Psychiatry J. – 1997. – 5, N 1. – P. 64 – 67.

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