Myelination and demyelinationprocesses in the rat cerebellum cellculture: an electron microscopic study.
T.A. Pivneva, E.V. Kolotushkina, G.G. Skibo
A.A.Bogomoletz Institute of Physiology National Academy ofSciences of Ukraine, Kiev
Abstract
We observed manifestations of the myelination process in
dissociated culture of the cerebellar tissue of newborn rats and
modifications of the structure of myelin sheaths after treating
the culture with a demyelinating factor, blood serum of patients
suffering from multiple sclerosis (MS). On day in vitro (DIV)
26, in the control myelin sheaths of the axons demonstrated
the closest resemblance to those observed in vivo, and we
selected this term for inducing demyelination. Addition of the
serum of MS patients to the culturing medium evoked rapid
(in 3-6 hours) dramatic changes in the ultrastructure of myelin
sheaths; these were a decrease in the number of the lamellae,
their splitting and invagination, formation of vesicles, etc. The
serum of MS patients in an acute stage of the disease exerted
more intensive demyelination effects than that of patients in a
remission stage.
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