Zhulinsky Comparative study of the effects ofcentral and conjunctive administrations of cholecystokinin to intact and diabetic rats
Y.M. Kolesnik, M.A. Orlovsky, A.V. Abrmov, V.A. Zhulinsky
Zhaporozhye State Medical University
Abstract
To investigate the role of cholecystokinin (ChCK) in the
pathogenesis of insulin-dependent diabetes mellitus (IDDM)
and to search the ways of its treatment with good prospects
we conducted a comparative study of the effects of chronic
conjunctive instillations (c.i.) ) and intracerebroventricular
administrations (i.c.v.) of cholecystokinin octapeptide (ChCК-8)
to intact and streptozotocin-induced IDDM rats. The
state of α – and β-cells in pancreatic islets was studied by
immunocytochemical method with a subsequent quantitative
analysis on an automatic image analysis system.
Our investigation has shown that in healthy rats both i.c.v.
and c.i. administrations of ChCK-8 induced a significant increase in glycemia due to stimulation of α-cells and
depression of β-cells. However effects of ChCK-8 on the synthesis
and secretion of insulin prevailed at i.c.v. administations,
while ChCК-8 administrated by c.i. was more potent in
stimulating α-cells. Both ways of ChCK-8 administrations
to a IDDM rats caused a positive effect on those animals by
inhibiting a destruction of β-cells, stimulating their function,
and decreasing the content of α-cells in pancreatic islets which
lead to a significant increase in insulin and a decrease in glucose in blood.
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