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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2003; 49(4): 72-79


Zhulinsky Comparative study of the effects ofcentral and conjunctive administrations of cholecystokinin to intact and diabetic rats

Y.M. Kolesnik, M.A. Orlovsky, A.V. Abrmov, V.A. Zhulinsky

    Zhaporozhye State Medical University


Abstract

To investigate the role of cholecystokinin (ChCK) in the pathogenesis of insulin-dependent diabetes mellitus (IDDM) and to search the ways of its treatment with good prospects we conducted a comparative study of the effects of chronic conjunctive instillations (c.i.) ) and intracerebroventricular administrations (i.c.v.) of cholecystokinin octapeptide (ChCК-8) to intact and streptozotocin-induced IDDM rats. The state of α – and β-cells in pancreatic islets was studied by immunocytochemical method with a subsequent quantitative analysis on an automatic image analysis system. Our investigation has shown that in healthy rats both i.c.v. and c.i. administrations of ChCK-8 induced a significant increase in glycemia due to stimulation of α-cells and depression of β-cells. However effects of ChCK-8 on the synthesis and secretion of insulin prevailed at i.c.v. administations, while ChCК-8 administrated by c.i. was more potent in stimulating α-cells. Both ways of ChCK-8 administrations to a IDDM rats caused a positive effect on those animals by inhibiting a destruction of β-cells, stimulating their function, and decreasing the content of α-cells in pancreatic islets which lead to a significant increase in insulin and a decrease in glucose in blood.

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