Zhulinsky Comparative study of the effects ofcentral and conjunctive administrations of cholecystokinin to intact and diabetic rats
Y.M. Kolesnik, M.A. Orlovsky, A.V. Abrmov, V.A. Zhulinsky
Zhaporozhye State Medical University
![](/images/cite.png)
Abstract
To investigate the role of cholecystokinin (ChCK) in the
pathogenesis of insulin-dependent diabetes mellitus (IDDM)
and to search the ways of its treatment with good prospects
we conducted a comparative study of the effects of chronic
conjunctive instillations (c.i.) ) and intracerebroventricular
administrations (i.c.v.) of cholecystokinin octapeptide (ChCК-8)
to intact and streptozotocin-induced IDDM rats. The
state of α – and β-cells in pancreatic islets was studied by
immunocytochemical method with a subsequent quantitative
analysis on an automatic image analysis system.
Our investigation has shown that in healthy rats both i.c.v.
and c.i. administrations of ChCK-8 induced a significant increase in glycemia due to stimulation of α-cells and
depression of β-cells. However effects of ChCK-8 on the synthesis
and secretion of insulin prevailed at i.c.v. administations,
while ChCК-8 administrated by c.i. was more potent in
stimulating α-cells. Both ways of ChCK-8 administrations
to a IDDM rats caused a positive effect on those animals by
inhibiting a destruction of β-cells, stimulating their function,
and decreasing the content of α-cells in pancreatic islets which
lead to a significant increase in insulin and a decrease in glucose in blood.
References
- Абрамов А.В. Пептидергічна система гіпотала муса в регуляції ендокринної функції підшлункової залози в нормі і при цукровому діабеті (експериментальне дослідження): Автореф. дис. … д-ра мед.наук. – К., 1998. – 25 с.
- Абрамов А.В., Колесник Ю.М., Тржецинский С.Д., М.А.Орловский. Изменения холецистокинин-синтезирующей системы гипоталамуса при экспе- риментальном сахарном диабете у крыс // Морфология. – 1998. – 144, № 6. – C.27 – 31.
- Василенко Г.В. Стан нейронів латерального гіпоталамічного поля і вентромедіального ядра при цукровому діабеті і його корекції за допомогою інтервальних гіпоксичних тренувань: Автореф. дис. … канд. мед. наук. – К., 1997. – 15 с.
- Громов Л.А. Нейропептиды.– К.: Здоров’я, 1992. –
- Луцик П.В., Калихевич В.Н., Ардемасова З.А. Импульсная активность нейронов вентромедиального гипоталамуса кролика при аппликации на роговицу глаза холецистокинина // Физиол. журн. им. Сеченова. – 1986. – №11. – C.1493 – 1496.
- Траилин А.В., Колесник Ю.М., Орловский М.А. Состояние NPY-синтезирующих клеток остров- ков Лангерганса у нормальных и диабетических крыс при введении синтетического нейропептида Y // Пробл. эндокринологии. – 2001. – 47, № 3. – C.36 – 40.
- Berthoud H.R., Fox E.A., Powley T.L. Identificaton of vagal preganglionics that stimulate insulin and glucagon secretion // Amer. J. Physiol. – 1990. – 258, № 2. – P.160 – 168.
- Bhavsar S., Watkins J., Young A. Synergy between amylin and cholecystokinin for inhibition of food intake in mice // Physiol. Behav. – 1998. – 64, № 4. – P.557 – 561.
- 9. Blevins E.J., Stanley G.B., Reidelberger R.D. Brain regions where cholecystokinin supresses feeding in rats // Brain Res. – 2000. – 860. – P.1 – 10.
- 10. Ervin G.N., Birkemo L.S., Johnson M.F., et al. The effects of anorectic and aversive agents on deprivation-in- duced feeding and taste-aversion conditioning in rats // J. Pharm. Exp. – 1995. – 273. – P.1203 – 1210.
- Felig P., Baxter J.D., Broadus A.E., Frohman L.A. Endocrinology and methabolism. – New-York; McGraw-Hill, 1995. –
- Jacoby R., Stafford D., Kouyama N., Marshak D. Syn- aptic inputs to ON parasol ganglion cells in the pri- mate retina // J. Neurosci. – 1996. – 16, № 24. – P.8041 – 8056.
- Jacoby R.A., Marshak D.W. Synaptic connections of DB3 diffuse bipolar cell axons in macaque retina // J. Comp. Neurol. – 2000. – 416, № 1. – P.19 – 29.
- Laychock S.G. Rat pancreatic-islet and RINM5F cell responses to epiandrosterone, dehydroepiandrosterone and interleukin-1-beta // Bioch. Pharm. – 1998. – 55. – P.1453 – 1464.
- Leibowitz S.F. Specificity of hypothalamic peptides in the control of behavioral and physiological processes // Ann. N-Y. Acad. Sci. – 1994. – 739, P.12 – 35.
- Matthews D.R., Clark A. Neural control of the endocrine pancreas // Proc. Nutr. Soc. – 1987. – 46, № 1. – P.89 – 95.
- Monnikes H., Lauer G., Bauer C. et al. Pathways of Fos expression in locus ceruleus, dorsal vagal com- plex, and PVN in response to intestinal lipid // Amer. J. Physiol. – 1997. – 273, № 6, Pt 2. – P.R2059 – R2071.
- Paxinos G.B., Watson C.C. The rat brain in stereotaxic coordinates. – Sydney: Acad. Press, 1986. –
- 19. Silver R., Sookhoo A.I., LeSauter J., et al. Multiple regu- latory elements result in regional specificity in circadian rhythms of neuropeptide expression in mouse SCN // Neuroreport. – 1999. – 10, № 15. – P.3165 – C.3174.
- 20. Swanson L.W., Sawchenko P.E. Hypothalamic integra- tion: Organization of the paraventricular and supraoptic nuclei // Ann. Rev. Neurosci. – 1983. – 6. – P.269 – 324.
|