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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2003; 49(4): 48-55

Insulin and endothelium-dependent mechanisms of regulation of coronary circula-tion

O.P. Neshcheret

    V.P. Komissarenko Institute of Endocrinology and Metabo-lism, Academy of Medical Sciences of Ukraine, Kyiv.



Abstract

Changes in cardiohemodynamics after intravenous administration of insulin (1.0 IU/kg) were investigated on anaesthetized dogs with closed chest. During the first 5-10 min (phase 1 of the response), coronary vasoconstriction, an enhancement of the cardiac activity and an increase in a coronary arteriovenous oxygen difference were observed. During the next 25-30 min (phase II) a long-term coronary dilation, an attenuation of the cardiac contractility and a decrease in the coronary arteriovenous oxygen difference occurred. An injection of insulin after preliminary inhibiting NO-synthase (L-MANE, intracoronary infusion, 1.0 mg/min), guanilatcy-clase (100 μg ouabaine, intracoronary injection), Na+,K+–AT-Pase (methylene blue, intracoronary infusion, 5 mg/min) induced coronary vasoconstriction. Inhibiting muscarinic receptors with atropine (0.5 mg/kg i.v.) attenuated or absolutely abolished haemodynamic responses induced by insulin. Under β-adrenergic blockade by propranolol (2.0 mg/kg i.v.) administrations of insulin resulted in coronary constriction, an augmentation of cardiac contractility and an elevation of the arterial blood pressure. The conception has being developed, according to which the degree and trends of induced by insulin changes in cardio-haemodynamics depend on the initial levels the sympathetic and parasympathetic activities, as well as on the synthesis of endothelial vaso- and cardiotropic substances, and their interactions with these systems.

Full text: (PDF, in Ukrainian)

References

  1. Нещерет О.П., Шепеленко І.В., Хомазюк А.І. Модуляція інсуліном адренергічних та холінергічних механізмів регуляції кровопостачання та функції серця // Журн. АМН України. – 2002. – 8, № 4. – С. 770 – 780.
  2. Фролькис В.В., Кобзарь А.А., Соколова О.В. Инверторный механизм гормональной регуляции Na+,K+- АТФазы // Бюл. эксперим. биологии и медицины. – 1996. – 122, № 7. – С. 9 – 11.
  3. Хомазюк А.И. Роль инсулина в регуляции кровооб- ращения и патогенезе болезней сердечно-сосудистой сис- темы // Укр.кардіол. журн. – 1999. – № 5. – С. 62 – 70.
  4. Хомазюк А.И. Экстракорпоральная перфузия и резистография коронарных сосудов у животных без вскрытия грудной клетки // Патол. физиология и эксперим. терапия. – 1986. – № 2. – С. 74 – 77 .
  5. Хомазюк А.І., Нещерет О.П., Шепеленко І.В. Кардіо- гемодинамічні та електрокардіографічні критерії захис- ної дії інсуліну при катехоламінових пошкодженнях серця // Доп. НАН України. – 2000. – № 12. – С. 198 – 203.
  6. Хомазюк А.І., Нещерет О.П., Шепеленко І.В. Вплив інсуліну на діяльність серця, вінцевий та системний кровообіг // Фізіол. журн. – 1994. – 40, № 3 – 4. – С. 3 – 9.
  7. Хомазюк А.И., Нещерет А.П., Яворский Л.А. Кате- тер. А. с. 659154 СССР // Открытия. Изобретения. – 1979. – № 16. – С. 14.
  8. Baron A.D. Vascular reactivity // Amer. J. Cardiol. – 1999. – 84, № 1A. – P. 25J – 37J.
  9. 9. Baron A.D. Hemodynamic action of insulin // Ibid. – 1994. – 267, № 2, Part 1. – P. E187 – E202.
  10. 10. Cardillo C., Nambi S.S., Kilcoyne C.M. et al. Insulin stimulates both endothelin and nitric oxide activity in the human forearm // Circulation. – 1999. – 100, № 8. – P. 820 – 825.
  11. Cleland S.J., Petrie J.R., Ueda S., Elliott H.L., Connell J.M. Insulin as a vascular hormone: implications for the pathophysiology of cardiovascular disease // Clin. Exp. Pharmacol. Physiol. – 1998. – 25, № 3 – 4. – P. 175 - 184.
  12. Ferranini E., Galvan A.Q., Gastaldelli A. et al. Insulin: new roles for ancient hormone // Eur.J. Clin. Invest. – 1999. – 29, № 10. – P. 842 – 852.
  13. Hauck C.M., Dunbar J.C. Effects of general and selective beta-adrenergic antagonists on insulin-induced cardiac and selected vascular responses in rats // Acta Diabetol. – 1999. – 36, № 1 – 2. – P. 53 – 60.
  14. Kawasaki H., Kuroda S., Mimaki Y. Vascular effects of insulin // Nippon Yakurigaku Zasshi. – 2000. – 115, № 5. – P. 287 – 294.
  15. Khalil Z., Marley P.D., Livett B.G. Elevation in plasma catecholamines in response to insulin stress is under both neuronal and nonneuronal control // Endocrinology. – 1986. – 118, № 1. – P. 150 – 157.
  16. Lembo G., Iaccarino G., Vecchione C. et al. Insulin modu- lation of an endothelial nitric oxide component present in the alpha2- and beta-adrenergic responses in human forearm // J. Clin. Invest. – 1997. – 100, № 8. – P. 2007 – 2014.
  17. Liang C.-S., Doherty J.U., Faillace R. et al. Insulin infusion in conscious dogs. Effects on systemic and coronary hemodynamics, regional blood flow, and plasma catecholamines // Ibid. – 1982. – 69, № 6. – P. 1321 –1336.
  18. McNulty P.H., Pfau S., Deckelbaum L.I. Effect of plasma insulin level on myocardial blood flow and its mechanism of action // Amer. J. Cardiol. – 2000. – 85, № 2. – P. 161 – 165.
  19. 19. Poldorman K.H., Stehouwer C.D.A., van Kamp G.J., Gooron L.J.G. Effects of insulin on endothelium-derived vasoactive substances // Diabetologia. – 1996. – 39, № 11. – P. 1284 – 1292.
  20. 20. Randin D., Vollenweider P., Tappy L. et al. Effects of adrenergic and cholinergic blockade on insulin-induced stimulation of calf blood flow in humans // Amer. J. Phys- iol. – 1994. –266, № 3. – P. R809 – R816.
  21. Scherrer U., Sartori C. Insulin as a vascular and sympathoexitatory hormone: Implication for blood pressure regulation, insulin sensitivity, and cardiovascular morbid- ity // Circulation. – 1997. – 96, № 11. – P. 4104 – 4113.
  22. Steinberg H.O., Brechtel G., Johnson A. et al. Insulinmediated skeletal muscle vasodilation is nitric oxide dependent. A novel action of insulin to increase nitric oxide release // J. Clin. Invest. – 1994. – 94, № 9. – P. 1172 – 1179.
  23. Tack C.J., Lutterman J.A., Vervoort G. et al. Activation of the sodium-potassium pump contributes to insulin- induced vasodilation in humans // Hypertension. – 1996. – 28, № 3. – P. 426 – 432.
  24. Yki-Yarvinen H., Utriainen T. Insulin-induced vasodilatation. Physiology or pharmacology? // Diabetologia. – 1998. – 41, № 4. – P. 369 –379.
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