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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2003; 49(4): 48-55


Insulin and endothelium-dependent mechanisms of regulation of coronary circula-tion

O.P. Neshcheret

    V.P. Komissarenko Institute of Endocrinology and Metabo-lism, Academy of Medical Sciences of Ukraine, Kyiv.


Abstract

Changes in cardiohemodynamics after intravenous administration of insulin (1.0 IU/kg) were investigated on anaesthetized dogs with closed chest. During the first 5-10 min (phase 1 of the response), coronary vasoconstriction, an enhancement of the cardiac activity and an increase in a coronary arteriovenous oxygen difference were observed. During the next 25-30 min (phase II) a long-term coronary dilation, an attenuation of the cardiac contractility and a decrease in the coronary arteriovenous oxygen difference occurred. An injection of insulin after preliminary inhibiting NO-synthase (L-MANE, intracoronary infusion, 1.0 mg/min), guanilatcy-clase (100 μg ouabaine, intracoronary injection), Na+,K+–AT-Pase (methylene blue, intracoronary infusion, 5 mg/min) induced coronary vasoconstriction. Inhibiting muscarinic receptors with atropine (0.5 mg/kg i.v.) attenuated or absolutely abolished haemodynamic responses induced by insulin. Under β-adrenergic blockade by propranolol (2.0 mg/kg i.v.) administrations of insulin resulted in coronary constriction, an augmentation of cardiac contractility and an elevation of the arterial blood pressure. The conception has being developed, according to which the degree and trends of induced by insulin changes in cardio-haemodynamics depend on the initial levels the sympathetic and parasympathetic activities, as well as on the synthesis of endothelial vaso- and cardiotropic substances, and their interactions with these systems.

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