Intracellular calcium homeostasis of sensoryneurons at hypoxic influences
P.G. Kostyuk1, 2, R.I. Stanika1, L.M. Koval1, E.A. Lukyanetz1, 2
- А.А.Bogomoletz Institute of Physiology, Kiev, Ukraine;
- International Center for Molecular Physiology, Kiev,Ukraine
Hypoxia is the main reason leading to neuronal death during
different forms of brain diseases. The main phenomenon observed at hypoxia is excessive growth of intraneuronal Ca2+
concentration leading to irreversible cell damage. Despite
extensive studies of this process, the intracellular mechanisms
responsible for disturbance in Ca2+ are still unclear. The aim of
present investigations was to explore these mechanisms. Ca2+
was measured by spatial screening of isolated dorsal root
ganglion (sensory) neurons loaded with fluorescent dye
Fura-2AM after exposing them hypoxic solution. Hypoxia
resulted in a reversible elevation of Ca2+
, which could be partly
prevented by several pharmacological agents. We concluded
that in sensory neurons hypoxia-induced elevation of cytosolic
is induced by primary changes in ionic channels and
secondary in function of mitochondria.
- Budd S.L. Mechanisms of neuronal damage in brain hypoxia/ischemia: focus on the role of mitochondrial calcium accumulation . Pharmacol.Ther. - 1998. - 80. - P. 203 - 229.
- Duarte C.B., Carvalho C.A., Ferreira I.L.,Carvalho A.P. Synaptosomal [Ca2+]i as influenced by Na+/Ca2+ exchange and K+ depolarization . Cell Calcium - 1991. - 12. - P. 623 - 633.
- Hammarstrom A.K., Gage P.W. Hypoxia and persistent sodium current . Eur.Biophys.J. - 2002. - 31. - P. 323 - 330.
- Konovalov A.A., Lukyanetz E.A. Activity of sodium voltage-operated currents of cortical neurones during hypoxia. Neurophysiology, 1998. 30. P. 312 - 315.
- Kostyuk P.G., Lukyanetz E.A., Doroshenko P.A. Effects of serotonin and cAMP on calcium currents in different neurones of Helix pomatia. Pflug. Arch. 1992. 420. P. 9 - 15.
- Kostyuk P.G., Shmigol A.V. Intracellular stores and calcium signalling in mammalian sensory neurones. Bioelectrochem Bioenerg. 1997. 42. P. 197 - 205.
- Lukyanetz E.A., Shkryl V.M. Scientific and technological aspects of oxygen-sensitive electrodes for measurements of oxygen partial pressure in patch-clamp experiments . J Biochem and Biophys Methods, 2003. 55. P. 37 - 52.
- Lukyanetz E.A., Sotkis A.V., Kostyuk P.G. Mechanisms of up-regulation of single calcium channels by serotonin in Helix pomatia neurons. Biochem Biophys Res Com 2002. 293. P. 132 - 138.
- Mironov S.L., Richter D.W. L-type Ca2+ channels in inspiratory neurones of mice and their modulation by hypoxia . J.Physiol. - 1998. - 512 ( Pt 1). - P. 75 - 87.
- Mironov S.L., Richter D.W. Hypoxic modulation of L-type Ca2+ channels in inspiratory brainstem neurones: intracellular signalling pathways and metabotropic glutamate receptors . Brain Res. - 2000. - 869. - P. 166 - 177.
- Orrenius S., Ankarcrona M., Nicotera P. Mechanisms of calcium-related cell death . Adv.Neurol. - 1996. - 71. - P. 137 - 149. 10 ISSN 0201-8489
- Shkryl V.M., Lukyanetz E.A. Properties of oxygensensitive electrodes using in patch-clamp experiments on nerve cells. Neurophysiology, 1998. 30. P. 279 - 283.
- Shkryl V.M., Kostyuk P.G., Lukyanetz E.A. Dual action of cytosolic calcium on calcium channel activity during hypoxia in hippocampal neurones. Neuroreport, 2001. 12. P.4035 - 4039.
- Shkryl V.M., Nikolaenko L.M., Kostyuk P.G.,Lukyanetz E.A. High-threshold calcium channel activity in rat hippocampal neurones during hypoxia. Brain Res. 1999. 833. P. 319 - 328.
- Solovyova N.A., Kostyuk P.G. Effects of nitric oxide and hypoxia on low- and high-voltage activated calcium currents in murine DRG neurons . Neurophysiology. 1999. 31. P. 69 - 72.
- Summers B.A., Overholt J.L.,Prabhakar N.R. Augmetation of calcium current by hypoxia in carotid body glomus cells . Adv.Exp.Med.Biol. - 2000. - 475. - P. 589 - 599.
- Thayer S.A., Miller R.J. Regulation of the intracellular free calcium concentration in single rat dorsal root ganglion neurones in vitro . J.Physiol - 1990. - 425. - P. 85 - 115.
- Vergun O., Sobolevsky A.I., Yelshansky M.V. et al. Exploration of the role of reactive oxygen species in glutamate neurotoxicity in rat hippocampal neurones in culture . Ibid - 2001. - 531. - P. 147 - 163.
- Werth J.L., Thayer S.A. Mitochondria buffer physiological calcium loads in cultured rat dorsal root ganglion neurons . J.Neurosci. - 1994. - 14. - P. 348 - 356.
- Zhang Y., Lipton P. Cytosolic Ca2+ changes during in vitro ischemia in rat hippocampal slices: major roles for glutamate and Na+-dependent Ca2+ release from mitochondria . Ibid. - 1999. - 19. - P. 3307 - 3315.