Intracellular calcium homeostasis of sensoryneurons at hypoxic influences
P.G. Kostyuk1, 2, R.I. Stanika1, L.M. Koval1, E.A. Lukyanetz1, 2
- А.А.Bogomoletz Institute of Physiology, Kiev, Ukraine;
- International Center for Molecular Physiology, Kiev,Ukraine
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Abstract
Hypoxia is the main reason leading to neuronal death during
different forms of brain diseases. The main phenomenon observed at hypoxia is excessive growth of intraneuronal Ca 2+
concentration leading to irreversible cell damage. Despite
extensive studies of this process, the intracellular mechanisms
responsible for disturbance in Ca2+ are still unclear. The aim of
present investigations was to explore these mechanisms. Ca 2+
was measured by spatial screening of isolated dorsal root
ganglion (sensory) neurons loaded with fluorescent dye
Fura-2AM after exposing them hypoxic solution. Hypoxia
resulted in a reversible elevation of Ca 2+, which could be partly
prevented by several pharmacological agents. We concluded
that in sensory neurons hypoxia-induced elevation of cytosolic
Ca 2+ is induced by primary changes in ionic channels and
secondary in function of mitochondria.
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