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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2003; 49(1): 67-75

Morpho-functional changes in circulating neutrophils at physiological and complicatedpregnancy

S.B. Koval

    Ін-т фізіології ім. О.О.Богомольця НАН України,Київ



Abstract

A comparative study of the morpho-functional characteristics of the circulating neutrophils was carried out by the electron microscopy method on women with physiological pregnancy and pregnancy-induced hypertension or EPH gestosis . Some leukocyte reactions were observed, and their intensity depended on the clinical manifestation of the EPH gestosis. In neutrophils there were detected reformation in the nucleus in the form of redistribution of the ratio between heterochromatin and euchromatin, as well as changes in the ultra-structure of nucleolemma. Both an amount of glycogen and initial or azurophil granules in the cytoplasm decreased very much but solubilization of their enzymes in cytosol did not occur. Such degranulation was due to the features of exocytosis which determined an extraleukocytic release of the content from the primary granules. The data obtained evidence that neutrophils are functionally active in the vascular bed and are likely to take part in the humoral regulation of the whole organism.

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References

  1. Бахов Н.И., Александрова Л.З., Титов В.Н. и др. Нейтро филы, их роль в регуляции метаболизма тканей //Усп. совр.биологии. – 1987. – 104,№ 2(5). – С.281 – 296.
  2. Большакова Т.Д. Активность симпатико – адреналовой системы как фактора риска развития болезней адапзации //Клин.лаб.диагностика. – 1997. – №5. – С.31.
  3. Бутенко З.Л., Глузман Д.Ф., Зак К.П. и соавт. Цитохимия и электронная микроскопия клеток крови и кроветворных органов. – К.:Наук.думка, 1974. – 248с.
  4. Вашкинель В.К., Петров М.Н. Ультраструктура и функ- ция тромбоцитов человека. – Л.:Наука, 1982. – 88 с.
  5. Галанкин В.Н., Токмаков А.М., Юнусходжаев Э.Х. Ультраструктурные проявления мембранной активности нейтрофильных лейкоцитов //Арх. патологии. – 1988. – 50, №7. – С.23 – 30.
  6. Коваль С.Б., Боярчук Е.Д. Некоторые этиопатогенетические особенности развития в организме распространенного ДВС-синдрома //Довкілля та здоров‘я. – 1999. – №1(8). – С.62 – 65.
  7. Коваль С.Б.. Луніна Н.В., Середенко М.М. Синдром дегрануляції нейтрофільних лейкоцитів //Буковин. мед. вісн. – 1998. – 2,№4. – С.204 – 213.
  8. Коваль С.Б., Середенко М.М., Луніна Н.В. Механізми впливу циркулюючих нейтрофільних гранулоцитів на реакцію вивільнення із тромбоцитів крові людини //Фізіол.журн. – 2001. – 47,№3. – С.26 – 34.
  9. 9. Коломийцева А.Г., Диденко Л.В. Преэклампсия – риск для матери и плода //Доктор. – 2001. – №3(7). – С.17 – 19.
  10. 10. Покровский А.А., Тутельян В.А. Лизосомы. – М.:Наука, 1976. – 382с.
  11. Ростомян М.А., Абрамян К.С. Ультраструктура клеток крови. – Ереван: Изд-во АН Арм.ССР. – 1975. – 152 с.
  12. Савельева Г.М., Шалина Р.И., Кашежева А.З. Значение ранней диагностики и терапии гестозов // Акушерство и гинекология. – 1989, №1. – С.73 – 76.
  13. Саркисов Д.С., Пальцын А.А.,Червонская Н.В. и др. Влияние сыворотки на внутри- и внеклеточную бактерицидную способность нейтрофилов //Арх. патологии. – 1988. – 50, №7 – С.17 – 23.
  14. Свечникова И.Г., Панин Л.Е., Маянская Н.Н. Влияние ингибиторов лизосомальной функции на изменение спектра гистоновых белков при физической наг- рузке у крыс //Тез.докл. III Всесоюз.симпоз.с международ. участием “Структура и функции лизосом”. Тбилиси; Москва, 1986. – С.177 – 178.
  15. Славинский А.А. Цитоплазматическая зернистость нейтрофильных лейкоцитов //Клин. лабор. диагностика. – 2002.– №3. – С.39 – 43.
  16. Шубич М.Г., Нагоев Б.С. Щелочная фосфатаза лейкоцитов в норме и патологии. – М.: Медицина,1980. – 224 с.
  17. Baggiolini M.,Dewald B. The neutrophil // Archs. Allerge appl. and Immunol. – 1985. – 76, № 1. – P.13 – 20
  18. Baggiolini M., Hirch J.G., de Duve C. Further biochemical and morphological studies of granule fractions from rabbit heterophil leukocytes // JCell Biol. – 1970. –45. – P.586 – 597.
  19. 19. Bahremand M., Schumacher H.R.Ir. Effect of medication on synvial fluid leukocyte differentials in patients with rheumatiod arthritis // Arthit. and Rheumat. – 1991. – 34, № 9. – P.1173 – 1176.
  20. 20. Bitensky L., Butcher R.C., Chayen J. Quantitative cytochemistry in the study of lysosomal function // Lysosomes in Biology and Pathology. – North-Holland, Amsterdam. – 1973 – Vol.3. – P.465 – 510.
  21. Bostock C.J., Sumner A.T. The eukaryotic chomosome. – NorthHolland: Pabl.Comp.Ams. – N.Y. – Oxf. – 1978. – 597 p.
  22. Bretz U., Baggiolini M., Biochemical and morphological characterization of azurophil and specific granules of human neutrophilic polymorphonuclear leucocytes // J.Cell Biol. – 1974. – 63. – P.251 – 269.
  23. Clark P., Boswell F., Greer I.A.The neutrophil and preeclampsia // Semin.Reprod. Endocr. – 1998. – 16, № 1. – P.57 – 64.
  24. De Bruyn W.C. A modifical OsO4 - (double) fixation procedure which selectively contrasts glycogen // Europ. IV Reg. Conf.EM. Rome. – 1968. – P.65 – 68.
  25. Ericsson J.L.E., Trump B.F. Observations on the application of electron microscopy of the lead phosphate technique for the demonstration of acid phosphatase // Histochem. – 1964 – 1965. – 4. – P.470 – 487.
  26. Graham R.C.Ir., Karnovsky M.J. The early stages of absorption of injected horseradish peroxidase in the proximaltubules of mouse kidney: ulrastructural cytochemistry by a new technique // J. Histochem. Cytochem. – 1966. – 14. – P.291 – 302.
  27. Halim A., Kanayama N., El Marandi E. et al. Immunohistological study in cases of HELLP syndrome (hemolysis, elevated liver enzymes and low platelets) and acute fatty liver of pregnancy // Ginecol. Obstetr. Invest. – 1996. – 41, № 2. – P.106 – 112.
  28. Hampton M.B., Kettle A.J., Winterbourn C.C. Inside the Neutrophil Phagosome: Oxidants, Myeloperoxidase and Bacterial Killing// J.Amer. Soc. Hematol. – 1998. – 92, № 9. – P.3007 – 3017.
  29. 29. Hayhoe F.G.J., Quaglino D. Haematological Cytochemistry. Churchill Livingstone. – Edinburgh, L., N.Y., 1980. – 320 c.
  30. 30. Hugon J.S., Borgers V. A direct lead method for the electron microscopic visualisation of alkaline phosphatase activity// J.Histochem.Cytochem. – 1966. – 14. – P.429 – 431.
  31. Jacques P.J. Endocytosis and the lysosomal apparatus: recent developments. In: Cell biogical aspects of disease. The plasma, membrane and lysosomes. – Boston – London, 1981. – P.151 – 169.
  32. Smolen J.E., Korchak H.M., Weissman G.Initial kinetics of lysosomal enzyme secretion and superoxide anion generation by human polymorphonuclear leukocytes// Inflam- mation. – 1980. – №4. – P.145 – 163.
  33. Tasca C. Introducere in morfologia cantitativa cito – histo- logica. – Bucuresti: Ed.Acad. R.S.R. – 1976. – 192 p.
  34. Watanabe I., Donahue S., Hoggatt N. Method for electron microscopic studies of circulating human leukocytes and observations on their fine structure// J.Ultrastr. Res. – 1967. – 20. – P.366 – 382.
  35. Williams D.J., de Swiet M. The pathophysiology of pre – eclampsia// Intensive Care Med. – 1997. – 23. – P.620 – 629.
  36. Wright D.G., Gallin J.I. Secretory responses of human neutrophils: exocytosis of specific (secondary) granules by human neutrophils during adherence in vitro and during exudation in vivo//J.Immunol.– 1979. – 123. – P.285 – 294.
  37. Zimmerman G.A., Prescott S.M., Mc Intyze T.M. Endot- helial cell interactions wish granulocytes: tethering and sig- naling molecules// Immunol. Today – 1992. – 13, №3. – P.93 – 100.
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