Українська Русский English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2003; 49(1): 38-45

The influence of hericium erinaceusextract on myelination process in vitro

E.V.kolotushkina, M.G.moldavan, K.yU.voronin, G.G.skibo

    A.A.Bogomoletz Institute of Physiology National Academy ofSciences, Kiev


Myelin sheathes, wrapping axons, perform the following important functions: support, protection, feeding and isolation. Injury of myelin compact structure leads to an impairment andsevere illness of the nerve system. Exact mechanisms underlying the myelination process and myelin sheaths damage have not established yet. Therefore search for substances, which provide regulatory and protective effects on the normal myelination as well as stimulating action on the remyelination after myelin damage, is of special interest. Recently it was shown that extract from mushroom Hericium erinaceus had activating action on the nerve tissue. So the aim of the present work was to study an influence of an extract from H. erinaceus on the cerebellar cells and the process of myelination in vitro. Obtained data revealed the normal growth of the nerve and glial cells with extract at cultivating. No pathologic or toxic action of the extract has been found. The cell ultrastructure was intact and similar to that observed in vivo. The process of myelination in the presence of the extract began earlier as compared to controls and was characterised by a higher rate. Thus, extract of H. erinaceus promoted normal development of cultivated cerebellar cells and demonstrated a regulatory effect on the process of myelin genesis process in vitro.


  1. Bozhkova V.P., Brezhestovsky P.D., Buravlev V.P. et al. Handbook on nerve tissue culture: methods, techniques, questions. - I.: Nauka, 1988. - 317 p.
  2. Roitbak A.I. Glia and its role in nerve system activity. - SPb: Nauka, 1993. - 351 p.
  3. Skibo G.G., Koval L.M. Structural features of neuron development in vitro. - K.: Nauk. Dumka, 1992. - 152 p.
  4. Khokhlov A.P., Savchenko Yu.N. Myelin and molecular basic of demyelination process // Korsakov J. Neuropathol. and Psychiatr. - 1990. - 90, N 8. - P. 104-109.
  5. Campagnoni A. Molecular biology of myelination. - In: Neuroglia. - New York: Oxford University Press, Inc., 1995. - P. 555-570.
  6. Casha S., Yu W.R., Fehlings M.G. Oligodendroglial apoptosis occurs along degenerating axons and is associated with FAS and p75 expression following spinal cord injury in the rat // Neuroscience. - 2001. - 103, N 1. - P. 203-218.
  7. Chang R. Functional properties of edible mushrooms // Nutr. Rev. - 1996. - 54 (11 Pt 2). - S91-3.
  8. Lee E.W., Shizuki K., Hosokawa S. et al. Two novel diterpenoids, erinacines H and I from the mycelia of Hericium erinaceum // Biosci. Biotechnol. Biochem. - 2000. - -64, N 11. - P. 2402-2405.
  9. 9. Fedoroff S., Richardson A. Protocols for neural tissue culture. - Totowa, New Jersey: Human Press, 1997. - 279 p.
  10. 10. Feldman M., Peters A. Ballooning of myelin sheaths in normally aged macaques // J. Neurocytol. - 1998. - 27. - P. 605-614.
  11. Kenmoku H., Shimai T., Toyomasu T. et al. A new erinacine from Hericium erinaceum, and its biosynthetic route to erinacine C in the basidiomycete // Biosci. Biotechnol. Biochem. - 2002. - 66, N 3. - P. 571-575.
  12. Ladiwala U., Lachance C., Simoneau S.J. et al. p75 neurotrophin receptor expression on adult human oligodendrocytes: signaling without cell death in response to NGF // J. Neurosci. - 1998. - 18, N 4. - P. 1297-1304.
  13. Moldavan M.G., Grodzinskaya A.A., Wasser S.P., Storozhuk V.M. Effects of higher basidiomycetes mushrooms on the hippocampal slices in rat // Int. J. Med. Mushrooms. - 1999. - 1. - P.173-180.
  14. Matthieu J-M., Honegger P., Trapp B.D. et al. Myelination in rat aggregating cell cultures // Neuroscience. - 1978. - 3. - P. 565-572.
  15. Nataf S., Naveilhan P., Sindji L. et al. Low affinity NGF receptor expression in the central nervous system during experimental allergic encephalomyelitis // J. Neurosci. Res. - 1998. - 52, N 1. - P. 83-92.
  16. Notterpek L.M., Bullock P.N., Malek-Hedayat S., Rome .H. Myelination in cerebellar slice culture: development of a system amenable to biochemical analysis // J. Neurosci. Res. - 1993. - 36, P. 621-634.
  17. Peters A., Palay S., Webster H. The fine structure of the nervous system. - New York: Oxford University Press, 1991. - 494 p.
  18. Waxman S.G., Black J.A. Axoglial interactions at the cellular and molecular levels in central nervous system myelinated fibers. In: Neuroglia. - New York: Oxford University Press, Inc., 1995. - P. 587-610. 19. Waxman S.C. Rules governing membrane reorganization and axon-glial interactions during development of myelinated fibers. In: Progress in Brain Research, Neural Regeneration. - New York: Raven Press, 1987. - 71. - P. 121-142.

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2018.