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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2003; 49(1): 3-12


Effect of inductors and inhibitors of the mitochondrial permeability transition pore (mpt) on its opening and release of unidentifiedmitochondrial factor

V.F. Sagach, G.L.. Vavilova, N.A. Strutinskaya, O.V. Akopova

    Ін-т фізіології ім. О.О.Богомольця НАН України,КиївІн-т фізіології ім. О.О.Богомольця НАН України,КиївІн-т фізіології ім. О.О.Богомольця НАН України,Київ


Abstract

The release of an unidentified substance (factor) induced by sulfhydryl group (SH) modifier and mitochondrial permeability transition pore (MPT) inductor phenylarsine oxide (PAO) has been found in vitro on isolated guinea-pig and rat heart mitochondria. The factor was also released at oxidative stress. The factor release induced by PAO was inhibited by cyclosporin A (CsA), the MPT inhibitor. Protective actions of antioxidants melatonin and trolox in vitro and in vivo, as well as dithiothreitol (DTT) and diethylmaleat (DEM) were studied. The influences of nitric oxide (NO) donor, sodium nitroprusside (SNP) on the mitochondrial factor release were also studied. We have shown the participation of this agent in the regulation of factor formation after inhibition of NO-synthase activity (NOS) by aminoguanidine. The data were obtained about MPT-opening after Ca2+ loading and under the influence of the MPT inductor PAO, inhibited by CsA. The results obtained on isolated mitochondria are in a good agreement with the those on the isolated guinea-pig heart after myocardial ischemia-reperfusion. It was concluded that mitochondria are essential for the factor formation and its release under ischemia-reperfusion of the myocardium.

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