Protection of heart from reperfusion injury andineffective oxygen using by inhibitors of the mitochondrial permeability transition pore
Sagach V.F., Shymanskaya T.V., Nadtochiy S.M.
A.A. Bogomoletz Institute of Physiology NAS Ukraine, Kiev
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Abstract
In experiments on isolated hearts of guinea-pig, perfused
under Langendorff preparation, possible protection of
hearts from reperfusion injury by the known inhibitors of
mitochondrial permeability transition pore - cyclosporin
A, and trolox - water-soluble vitamin E was studied. It has
been shown that cardiac reperfusion was followed with an
increase in an oxygen cost of myocardial work (by 83%
from control level in 40 min of reperfusion), in addition to
the disturbances of cardiac contractility, tone of the coronary vessels and heart rate. The heart function and myocardial oxygen metabolism disturbances, due to global
20 min ischaemia and reperfusion, were essentially
decreased by a preliminary application of investigated
agents. Trolox improved cardiac recovery both when it
was perfused in vitro and after its administration per os.
In 40 min of heart reperfusion LVdeveloped pressure was
79% as compared to 51% in that at control; dР/dt max and
dР/dt min were 88% and 85% accordingly against 66% and
45% in control; oxygen cost of myocardial work didn“t
change reliably). Conclusion: postreperfusion disturbances
of cardiac contractility, tone of the coronary vessels and
heart rate, as well as noneffective oxygen utilization by the
heart tissue were due to an opening of mitochondrial permeability transition pore.
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