Elevated temperatures negatively impact rabbits’ metabolism, leading to oxidative stress and their death. To solve this problem, organic compounds of trace elements produced by nanotechnology, which are characterized by high absorption, bioavailability, and surface activity, which ensures interaction with the cellular structures of the body, are being studied in scientific practice. The aim of our study was to determine changes in the antioxidant defense of rabbits after feeding zinc, selenium and germanium citrate nanoparticles under moderate heat stress. The study was conducted on young rabbits of the Thermon White breed from the 35th to the 78th day of life. Rabbits were kept in a vivarium at an elevated temperature of 27.8 to 28.9 °C and relative humidity of 83.4 to 87.7 %. Rabbits of experimental groups I, II, and III consumed the same feed and water without restrictions as the animals of the control group, but received water for 24 h: Group I - 60 mg/l zinc citrate; Group II - 300 µg/l selenium citrate; Group III - 62.5 µg/l germanium citrate. Blood for the study was taken on the 14th day of the preparatory period and the 14th and 29th days of the experimental period. During these periods, the temperature and humidity index were determined to comply with the conditions of heat stress. Thus, it was found that additional feeding of zinc citrate and selenium citrate was characterized by positive changes in the functioning of the antioxidant defense system of rabbits: it reduced the content of lipid hydroperoxides and TBA-active products, increased the activity of superoxide dismutase, catalase, reduced glutathione and glutathione peroxidase in the blood on the 14th and 29th day of the study.