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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2002; 48(5): 17-21

Elastolitic system of pancreas at modellingdiabetes melitus

M.V. Borysyuk, V.Ye. Dosenko, Yu.V. Byts

    A.A.Bogomoletz Medical University, Kiev



Abstract

At modelling streptozotocin and alloxan diabetes melitus in rats we have detected an imbalance between elastase and its inhibitors (alpha 1 inhibitor of protein kinases, alpha-2 macroglobulin, acid-thermoresistant inhibitors) in tissues of the pancreas. The contribution of proelactase decreased but that of an active form of protein kinase increased; the content of alpha 1 inhibitor of protein kinase and alpha 2 macroglobulin reduced, however the contribution of the acid-thermoresistant inhibitors in the pancreatic tissues in- creased. Imbalance in the system elastase- inhibitors, shown at the model of diabetes mellitus, may be an important mechanism of an exocrine unsufficiency of the pancreas which results in pancreatitis.

Keywords: diabetes melitus, rats

Full text: (PDF, in Ukrainian)

References

  1. Балаболкин М.И. Эндокринология. – М.: Медицина, 1998.–400 с.
  2. Баранов В.Г. Экспериментальный сахарный диабет. – Л.:Наука, 1983.–240 с.
  3. Биць Ю.В., Досенко В.Є. Роль еластази в патогенезі артеріосклерозу // Пробл. медицини.1999.–№5.–С.10–17.
  4. Веремеенко К.Н. Голобородько О.П., Кизим А.Й. Протеолиз в норме и при патологии. – К.: Здоров’я, 1988.–200с.
  5. Веремєєнко К.М., Кізім А.Й., Досенко В.Є. Альфа2-макроглобулін: структура, фізіологічна роль і клінічне значення// Лаб. діагностика.–2000.–№2.–С.3–8.
  6. Веремеенко К.Н., Кизим А.И., Терентьев А.Г. Определение активности еластазы и ее ингибиторов в сыворотке крови с помощью хромогенных субстратов // Клин. лаб. диагностика.–1992.–№5–6.–C.58–61.
  7. Логинов А.С. Ингибиторы протеаз в поджелудочной железе и сыворотке крови: физиологическая роль // Физиол.журн.–1982.– 28, №5. – С.692–698.
  8. Морозова Н.Н. Внешнесекреторная функция поджелудочной железы при начальных формах сахарного диабета // Клин. медицина. – 1980.–58, №6. – С.68–75.
  9. 9. Нартикова В.Ф. Термо-кислотостабильный ингибитор трипсина из сыворотки крови кролика: выделение, очистка и свойства: Автореф. дис.... канд.биол.наук.– М., 1970.– 27 с.
  10. 10. Системная энзимотерапия. Теоретические основы, опыт клинического применения /Под ред. К.Н.Веремеенко. – К.: Морион, 2000. – 320с.
  11. Bernstein P.R., Edwards P.D., Williams J.C. Inhibitors of human leukocyte elastase [Review]// Progress. Med. Chem.– 1994.– 31.–P. 59–120.
  12. Kato M., Hayakawa S., Naruse S. et al. Plasma alpha 2-macroglobulin-trypsin complex like substance (MTLS) in pancreatic disease //J. Clin.Lab. Anal. –1996.–10, №6.–P.399–402.
  13. Li Z., Zhao L., Sandler S. et al. Expression of pancreatic islet MHC class I, insulin, and ICA 512 tyrosine phosphatase in low-dose streptozotocin-induced diabetes in mice // J. Histochem.Cytochem.–2000.–48, №.67.–P.761–767.
  14. Lorini R., Cortona L., Scotta M.S. et al. Exocrine pancreatic function in children and adolescents with insulin-dependent diabetes mellitus // Diabetes.Res. Clin. Pract.–1990.–8, № 3. – P.263–267.
  15. Lowry O.H., Rosenbrough N.J., Farr A.L. et al. Protein measurement with Folin phenol reagent // J. Biol. Chem.–1951.–193.–P.265–275.
  16. Lysiak J.J., Hussaini I.M., Webb D.J. et al. Alpha 2-macroglobulin function as a cytokine carrier to induce nitric oxide syntesis and cause nitric oxidedependent cytotoxicity in the RAW 264.7 macro-phage cell line // J. Biol. Chem.– 1995.– 270,№ 37.– P. 21919–21927.
  17. Mashiba S., Wada Y., Mayama H. et al. In vivo complex formation of oxidized а1-antitrypsin and LDL // Arterioscler. Thromb. Vasc. Biol.– 2001.–21, №11.–P.1801–1809.
  18. Sato M, Yamamoto K, Mayama H. et al. Exocrine pancreatic function in diabetic children //J. Pediatr. Gastroenterol. Nutr.–1984.–3, № 3.–P.415–420.
  19. 19. Umans L, Serneels L, Stas L. et al. Alpha2–macrodlobulin and murinodlobulin-1-deficient mice. A mouse model for acute pancreatitis //Pancreas.– 1998.–17, № 3.– P.289–294.
  20. 20. Wood S.C., Rao T.D., Frey A.B. Multidose strep- tozotocin induction of diabetes in BALB/c mice induces a T cell proliferation defect in thymocytes which is reversible by interleukin-4 // Cell.Immunol.–1999.–192, № 1.–P.1–12.
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