Increased nitric oxide stable metabolites blood level in highlanders
V.F. Sagach 1 , L.B. Doloman 1 , A.V. Kotsuruba 2 , О.М. Buhanevich 2, H.А. Kurdanov 3 , І.А. Beslaneev 3 , S.А. Bekuzarova 3
- A. A. Bogomoletz Institute of Physiology NASU, Kiev, Ukraine;
- A.V. Palladin Institute of Biochemistry NASU, Kiev, Ukraine;
- International Centre for Astronomical, Medical and Ecological Research NASU, RAS and government of KBR, Terskol, Russia
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Abstract
The investigation was designed to study nitric oxide
(NO) blood level estimated by measuring its stable
metabolites nitrite (NO 2-) and nitrate (NO 3-) concentration in highlanders (2,200 m a.s.l.). NO2- и NO3-
concentration was measured with Griss reagent and
brucine reagent respectively with using the spectrophotometric method. We first found that high-
landers compared to lowlanders have increased blood
level of NO 2- (37 ± 3.3 nmol/ml vs. 28.5 ± 1.3 nmol/ml,
Р < 0.05) and NO3- (1362 ± 43 nmol/ml vs. 845 ±
65 nmol/ml, Р < 0.001). As the most significant,
there was increase in NO 2- concentration in erythrocytes (by about 6 times), NO3- level in erythrocytes
was revealed to be increased by 2.5 times, whereas the
plasma concentration of these stable metabolites was
the same (for NO 3-) or even slightly decreased (for
NO2-). Such increased NO stable metabolites blood
level in highlanders may explain widening of vasodilative reserve and strengthening of cardiac pump
and contractile functions, which we revealed previously. Activation of NO-system in high altitude is
important and probable trigger adaptive mechanism
of cardiovascular system to hypoxia. This mechanism
allows to retain adequate tissue perfusion and optimal
oxygen supply in its decreased partial pressure in
inhaled air and thereby to prevent diseases caused by
vasospastic processes.
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