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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2002; 48(5): 3-8


Increased nitric oxide stable metabolites blood level in highlanders

V.F. Sagach 1 , L.B. Doloman 1 , A.V. Kotsuruba 2 , О.М. Buhanevich 2, H.А. Kurdanov 3 , І.А. Beslaneev 3 , S.А. Bekuzarova 3

  1. A. A. Bogomoletz Institute of Physiology NASU, Kiev, Ukraine;
  2. A.V. Palladin Institute of Biochemistry NASU, Kiev, Ukraine;
  3. International Centre for Astronomical, Medical and Ecological Research NASU, RAS and government of KBR, Terskol, Russia


Abstract

The investigation was designed to study nitric oxide (NO) blood level estimated by measuring its stable metabolites nitrite (NO2-) and nitrate (NO3-) concentration in highlanders (2,200 m a.s.l.). NO2- и NO3- concentration was measured with Griss reagent and brucine reagent respectively with using the spectrophotometric method. We first found that high- landers compared to lowlanders have increased blood level of NO2- (37 ± 3.3 nmol/ml vs. 28.5 ± 1.3 nmol/ml, Р < 0.05) and NO3- (1362 ± 43 nmol/ml vs. 845 ± 65 nmol/ml, Р < 0.001). As the most significant, there was increase in NO2- concentration in erythrocytes (by about 6 times), NO3- level in erythrocytes was revealed to be increased by 2.5 times, whereas the plasma concentration of these stable metabolites was the same (for NO3-) or even slightly decreased (for NO2-). Such increased NO stable metabolites blood level in highlanders may explain widening of vasodilative reserve and strengthening of cardiac pump and contractile functions, which we revealed previously. Activation of NO-system in high altitude is important and probable trigger adaptive mechanism of cardiovascular system to hypoxia. This mechanism allows to retain adequate tissue perfusion and optimal oxygen supply in its decreased partial pressure in inhaled air and thereby to prevent diseases caused by vasospastic processes.

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