Українська English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2002; 48(4): 75-80

Electrical responses of aortic endotheliumin spontaneously-hypertensive rats

A.I. Bondarenko V.F. Sagach

    A.A.Bogomoletz Institute of PhysiologyNational Academy of Science of Ukraine, Kiev


Endothelium-dependent relaxation is known to be depressed in arteries of SHR. We studied acetylcholine (Ach) -induced electrical responses of intact aortic endothelial cells from WKY rats and SHR using perforated patch-clamp method. Resting Em averaged -44,3±1,1 mV and -35,2±1,2 mV for WKY and SHR, respectively. Application of K+ free solution depolarized endothelium by 11,0±0,7 mV and 7,5±1,3 mV (р<0,05) for WKY and SHR, respectively, showing inhibition of endothelial electrogenic Na+/K+ pump in SHR. The amplitude of Ach-induced hyperpolarization for WKY and SHR was 19±1 mV and 25±1 mV, respectively. However, the rate of its recovery was different within particular preparation taken from SHR whereas it was very similar within particular preparation from WKY rats, showing alterations in intercellular signaling in endothelium of SHR.


  1. Бондаренко А.И., Сагач В.Ф. Модуляция мембранного потенциала клеток интактного эндотелия аорты морской свинки // Нейрофизиология.-1996.-28, N6.- С.260-266.
  2. Ткаченко М.М., Яроцький В.В., Марченко С.М.,Сагач В.Ф. Вплив ацетилхоліну та аденозинтрифосфату на мембранний потенціал інтактого ендотелію аорти щурів за умов старіння //Фізіол. журн.-2002.-48, N3.-С.3-8.
  3. Busse R., Fichtner H., Luckhoff A., Kohlhardt M.,Hyperpolarization and increased free calcium in acetylcholine-stimulated endothelial cells //Amer. J. Physiol.- 1988.- 255.-H965-H969.
  4. Cox R.H., Lozinskaya I., Dietz N.J. Differences in K+ current components in mesenteric artery myocytes from WKY and SHR //Amer. J. Hypertens.-2001.- 14, N9. P.897-907.
  5. Dalle Lucca J.J., Borges A.C., Ponchirolli R., et al. Role of smooth muscle cell membrane potential in neointima formation in arteries of spontaneously hypertensive rats // Pathophysiology.- 2001.- 7,N4.-P.245-250.
  6. Daut J., Mehrke G., Nees S., Newman W.H. Passive electrical properties and electrogenic sodium transport of cultured quinea pig coronary endothelial cells // J. Physiol.-1988.-402.-P.237-254.
  7. Gauthier K.M.,. Rusch N.J. Rat coronary endothelial cell membrane potential during hypertension // Hypertension.-2001.-37.-P.66-71.
  8. Himmel H.M., Whorton A.R., Strauss H.C. Intracellular calcium, currents, and stimulus-response coupling in endothelial cells // Ibid.- 1993.- 21.-P.112-127.
  9. 9. Kohler R., Distler A., Hoyer J. Increased mechanosensitive currents in aortic endothelial cells from genetically hypertensive rats // J. Hypertens.-1999.- 17, N3,-P.365-371.
  10. 10. Luscher R.F., Vanhoutte P.M. Endothelium-dependent contraction to acetylcholine in the aorta of the spontaneously hypertensive rat // Hyper-tension.-1986.-8.-P.344-348.
  11. Manjeet S., Sim M.K. Decreased Na+K+ATPase activity in the aortic endothelium and smooth muscle of the spontaneously hypertensive rats //Clin. Exp. Hypertens.-1987.-9, N4. - P.797-812.
  12. Marchenko S.M., Sage S.O. Electrical properties of resting and acetylcholine-stimulated endothelium in intact rat aorta // J.Physiol.-1993.- 462,N3.-P.735-751.
  13. Marin J., Redondo. Vascular sodium pump: endothelial modulation and alterations in some pathological processes and aging // Pharmacol. and Therapeut.-1999.-84.-P.249-271.О.І. Бондаренко, В.Ф. Сагач
  14. Martens J.R., Gelband C.H. Alterations in rat interlobar artery membrane potential and K+ channels in genetic and nongenetic hypertension //Circulat. Res.- 1996.-79.-P.295-301.
  15. Nilius B., Viana F., Droogmans G. Ion channels in vascular endothelium // Annu. Rev. Physiol. –1997.-59.-P.145-170.
  16. Rusch N.J, De Lucena R.G, Wooldridge T.A, et al. Ca2+-dependent K+ current is enhanced in arterial membranes of hypertensive rats // Hypertension.-1992.-19.-P.301-307.
  17. Sohn H.-Y., Keller M., Gloe T., et al. The small G-protein Rac mediates depolarization-induced superoxide formation in human endothelial cells// J. Biol. Chem.-2000.- 275.-P.18745-18750.
  18. Soloviev A.I., Stefanov A.V., Bazilyuk O.V, Sagach V.F. Phospholipid vesilcles (liposomes) restore endothelium-dependent cholinergic relaxation in thoracic aorta from spontaneously-hypertensive rats //J. Hypertension.– 1993.- 11.-P.623-627.
  19. 19. Vanhoutte P.M. Endothelial dysfunction in hypertension // Ibid.- 1996.-14.- S83-S93.
  20. 20. Wellman G.C., Cartin L., Eckman D.M., et al.,Membrane depolarization, elevated Ca(2+) entry,and gene expression in cerebral arteries of hypertensive rats //Amer. J. Physiol. Heart Circulat. Physiol.-2001.- 281, N6.- H2559-2267.
  21. Zharikov S.I., Herrera H., Block E.R. Role of membrane potential in hypoxic inhibition of L-arginine uptake by lung endothelial cells//Amer. J. Physiol.- 1997.-272, N1,Pt 1.-L78-84

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2024.