The effect of progesterone and 17β-estradiolon proinflammatory cytokine costimulatory proliferative activity
S.P. Grekova M.A. Vodyanik V.P. Chernyshov
Institute of Pediatrics, Obstetrics and Gynecology,Academy of Medical Sciences, Kiev
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Abstract
The lymphocyte proliferation is multicomponent mechanism of immune system reactivity.
Many costimulatory factors take part in this process. Proinflammatory
cytokines (TNF, IL-1α and β) enhance proliferation of activated lymphocytes. Female steroid
hormones inhibit proliferation of mitogen and alloantigen–activated
lymphocytes. The aim of this study
was to investigate the effect of progesterone and 17β-estradiol on the costimulatory proliferative activity
of proinflammatory cytokines in vitro. Female steroid
hormones inhibit lymphocyte response to antiCD3
antibody. Progesterone had a stronger effect than 17β-estradiol (64 and 13% of inhibition respectively).
17β-estradiol enhanced the TNF costimulatory effect
on the lymphocyte proliferation. Progesterone neutralized this TNF-induced effect and reverted it (inhibition of lymphocyte proliferation was enhanced in
the presence of TNF). We found dominant inhibitory
effect of progesterone on the TNF costimulatory activity when progesterone and estrogen were added simultaneously.
Progesterone and 17β-estradiol downregulated costimulatory proliferative activity of IL-1 α
or α. Thus female steroid hormones had suppressive
effect on the antiCD3-stimulated lymphocyte proliferation. They downregulated costimulatory proliferative activity of IL-1 α/β and had opposite effect
on TNF costimulatory activity. Our results suggest
possible roles female steroid hormones as regulators
on activity of proinflammatory cytokines and their
functions in lymphocyte proliferation.
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