THE METHOD OF EVOKED POTENTIALS AS A PROMISING DIRECTION FOR THE STUDY OF NOCICEPTION IN ANESTHETIZED ANIMALS
D. Zavodovskyi1, O. Lehedza1, N. Bulgakova1, N. Semenuk2, О. Kostyukov1
- Bogomoletz Institute of Physiology of NAS of Ukraine, Kyiv, Ukraine
- National University of “Kyiv-Mohyla Academy”, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz70.03.065

Abstract
The sensation of pain is a pathogenetic link in a wide range
of diseases, and the study of nociception as its component
is an important area of physiology and medicine. However,
modern requirements for research conducted using laboratory
animals require the search for new approaches to studying
nociception and analgesia with minimizing the suffering of
subjects in ethological testing and maximizing the transition
to instrumental testing. We proposed the use of a standard
formalin model of pain in combination with the recording
of changes in somatosensory cortex potentials caused by
electrical stimulation of efferents at the level of the forelimb.
It was found that after subcutaneous injection of 0.30 ml of
4% formalin, the amplitude of evoked potentials increased.
Under the conditions of the proposed testing design, both dosedependent and time-dependent effects of the formalin model of
pain were observed in an anesthetized animal. The peculiarity
of the obtained results was that the fixed somatosensory
potentials of the brain in the S1 forelimb region showed
sensitivity to the administration of a nociceptive agent in those
areas of the body that topographically did not belong to the cortical representation of the forelimbs. This probably indicates
the potential universality of this test approach. Thus, it was
shown that evoked potentials in the forelimb representation
of the primary somatosensory cortex demonstrate a clearly
fixed response to painful stimulation in the formalin model of
pain and can be used in studies of nociceptive and analgesic
effects as a partial alternative to standard ethological testing.
Keywords:
pain; nociception; formalin test; evoked potentials; somatosensory cortex.
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