ROLE OF POTASSIUM CHANNEL TREK-1 IN MECHANOSENSITIVITY OF SMOOTH MUSCLE CELLS FROM RAT DETRUSOR
S.I. Yeliashov, B.R. Sharopov, Ya.M. Shuba
Bogomoletz Institute of Physiology National Academy of
Sciences of Ukraine, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz70.02.035
Abstract
Currently, TREK-1 is considered to be the main mechanosensitive channel in detrusor smooth muscle (DSM) cells. The aim
of our study was to detect the functioning of the K+-conducting
mechanosensitive TREK-1 channel in rat DSM cells using
the patch-clamp technique in response to hydrodynamic
stimulation (shear stress) and to determine the effects of a
TREK-1 agonist – arachidonic acid (AA) and an antagonist
– L-methionine. Mechanical stimulation of DSM cells using
hydrodynamic stress led to the appearance of a membrane
current with signs of pronounced outward rectification at
positive membrane potentials, which is typical of TREK-1
activation. The application of AA (50 mcmol/l) activated a
current with similar characteristics of the outward rectification
to the shear stress-activated one. L-methionine (10 mcmol/l)
almost completely prevented the generation of an outwardly
rectifying current in response to shear stress stimulation. DSM
cells also retained the ability to generate a mechanoactivated
current with a more pronounced inward component when
extracellular and intracellular K+ were replaced by Cs+. It was
concluded that the dominant mechanoactivated current in rat
DSM cells is carried by K+-selective TREK-1 channels, but a
small portion of this current can also be carried by other nonselective mechanosensitive cation channels.
Keywords:
mechanosensation; urinary bladder; smooth muscle cells; shear stress; TREK-1.
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