CONTENT OF GROWTH FACTORS AND HYPOXIA-INDUCIBLE FACTOR 1Α IN THE WOUND BED OF THE SKIN OF RATS WITH METABOLIC SYNDROME
N.R. Hrytsevych1, N.S. Nikitina2, L.I. Stepanova2, O.M. Savchuk2, V.V. Vereshchaka2
- Higher Educational Communal Institution of Lviv Regional
Council “Andrey Krupynsky Lviv Medical Academy”, Lviv, Ukraine
- Educational and Scientific Center “Institute of Biology
and Medicine” of Taras Shevchenko National University of
Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz69.06.069
Abstract
Minor injuries in healthy people usually heal well, but larger
wounds or the presence of various physiological (age) or
pathological conditions (metabolic syndrome, obesity, diabetes, and cancer) can impede this process. The aim of our work
was to determine the factors that may influence the duration
of healing (growth factors and hypoxia-induced factor 1α) in
the wound bed of rats with metabolic syndrome. The experiments were conducted on 80 white non-linear laboratory rats,
aged 4-5 months, which were divided after birth into 2 groups
of 40 animals each (20 males and 20 females). Group I rats
were subcutaneously injected with saline at a dose of 8 μg/
ml on days 2, 4, 6, 8, and 10 after birth. Group II rats were
administered a sodium glutamate solution at a dose of 4.0 mg/
kg at the same time. At the age of 4 months, animals of both
subgroups were modeled with incised wounds . The control
animals were rats in each of the groups in which wounds
were not modeled. The material for biochemical studies was
the skin in the areas of the former wound bed. Rats in the
control group had their skin excised at the same sites as those
in the experimental groups. The skin was homogenized and
the content of growth factors of endothelial and nerve cells
(VEGF, NGF, respectively) and hypoxia-inducible factor
(HIF-1α) was determined by immuno-enzymatic method.
In unoperated male rats with metabolic syndrome, the skin
content of VEGF, NGF, and HIF-1a increased compared to
control animals without the syndrome. In unoperated females
with metabolic syndrome, VEGF levels decreased with a
simultaneous increase in NGF and HIF-1α. In the wound bed
of animals with metabolic syndrome, after the closure of the
wound surface, the content of VEGF and HIF-1α increased,
and the content of NGF remained unchanged compared with
the values in unoperated rats. The results obtained indicate the
involvement of growth factors VEGF and NGF and HIF-1α in
prolonging the duration of healing of incised wounds in rats
with metabolic syndrome. At the same time, these growth
factors and HIF-1α may be involved in the mechanisms of
development of some postoperative complications.
Keywords:
cut wounds; skin; metabolic syndrome; growth factors; hypoxia-inducible factor 1α.
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