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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2023; 69(5): 66-74


V.V. Lomako

    Institute for Problems of Cryobiology and Cryomedicine of the NAS of Ukraine, Kharkov, Ukraine


It has been suggested that the preventive administration of cryopreserved cord blood nucleated cells (CBNCs) prior to the onset of desynchronosis may help to correct its negative effects on the body. The efficacy of CBNCs on blood leukocyte indicators in young and old rats (6 and 18 months old) was investigated. Desynchronosis was modelled by shifting the light regime: the duration of the light period was increased by 12 h, resulting in a light period of 24 h. Leukocyte types were determined in blood smears. Integral leukocyte indices were used to assess the state of the immune system. Desynchronosis caused leukocytosis in young rats and leukopenia in aged rats. The number of banded neutrophils increased in both, segmented neutrophils decreased in young rats and increased in aged rats; lymphocytes changed on the contrary; eosinophils decreased in young rats but did not change in aged rats. In young rats with desynchronosis, the predominance of young cells, macrophages, activation of the humoral link of the immune system, auto- and endogenous intoxication, acceleration of hypersensitivity of the immediate type processes, and increased body adaptation were noted. In aged rats, infectious intoxication, cellular link of immune system predominance, and a decrease in allergy and adaptation were observed. After CBNCs injection before desynchronosis, leukocytosis remained, segmented neutrophils and eosinophils recovered, banded neutrophils increased and lymphocytes decreased in young rats. In old rats, the total number of leukocytes, monocytes and eosinophils recovered, but lymphopenia increased. Regardless of age, the cells of non-specific protection predominated; the cellular link of the immune system activated, adaptation decreased and autointoxication was manifested. In young rats, young forms of neutrophils increased, allergy and immunoreactivity decreased, and the affective link of the immune system and macrophage in aged rats was activated. Therefore, after the preventive use of human CBNCs before desynchronosis initiation in aged rats, the total leukocyte content and the number of eosinophils and monocytes were restored. In 6-month-old rats, the content of young forms of neutrophils increased, indicating stimulation of leukopoiesis. The allergy index decreased in both groups.

Keywords: blood leukocytes; desynchronosis; cryopreserved human cord blood nucleated cells; age; rats.


  1. Bazhanova ED. Desynchronosis: types, main mechanisms, role in the pathogenesis of epilepsy and other diseases: a literature review. Life (Basel). 2022 Aug 11; 12(8):1218. CrossRef PubMed PubMedCentral
  2. Steele TA, St Louis EK, Videnovic A, Auger RR. Circadian rhythm sleep-wake disorders: a contemporary review of neurobiology, treatment, and dysregulation in neurodegenerative disease. Neurotherapeutics. 2021; 18(1):53-74. CrossRef PubMed PubMedCentral
  3. Berglund S, Magalhaes I, Gaballa A, Vanherberghen B, Uhlin M. Advances in umbilical cord blood cell therapy: the present and the future. Exp Opin Biol Ther. 2017; 17(6): 691-99. CrossRef PubMed
  4. Orlando N, Pellegrino C, Valentini CG, Bianchi M, Barbagallo O, Sparnacci S, Forni F, Fontana TM, Teofili L. Umbilical cord blood: Current uses for transfusion and regenerative medicine. Transfus Apher Sci. 2020; 59(5):102952. CrossRef PubMed
  5. Babiichuk LO, Grischenko VI, Gurina TM, et al., inventors; Institute for Problems of Cryobiology and Cryomedicine, assignee. [Method of cryopreservation of nucleated cord blood cells, including hematopoietic stem cells]. Patent of Ukraine No. 92227. 11.10.2010. [Ukrainian].
  6. Omman RA, Kini AR. Leukocyte Development, Kinetics, and Functions Chapter 9. In: Keohane EM, Walluga JM, Otto CN. Rodax's hematology. Clinical principles and applicatios. 6th ed. Elsevier; 2020. 117-35. CrossRef
  7. Dale DC, Boxer L, Liles WC. The phagocytes: neutrophils and monocytes. Blood. 2008; 112(4): 935-45. CrossRef PubMed
  8. Kolaczkowska E, Kubes P. Neutrophil recruitment and function in health and inflammation. Nat Rev Immunol. 2013; 13(3): 159-75. CrossRef PubMed
  9. Sudo A, Miki K. Circadian rhythm of catecholamine excretion in rats after phase shift of light-dark cycle. Indust Health. 1995; 33(2): 57-66. CrossRef PubMed
  10. Dong CH, Wang ZM, Chen SY. Neutrophil to lymphocyte ratio predict mortality and major adverse cardiac events in acute coronary syndrome: A systematic review and meta-analysis. Clin Biochem. 2018; 52: 131-6. CrossRef PubMed
  11. Speransky AI, Samoilenko GE, Lobachev MV. General blood test - are all its possibilities exhausted? Integral intoxication indices as criteria for assessing the severity of endogenous intoxication, its complications and the effectiveness of the treatment. Health Ukraine. 2009; 19 (6): 51-7.
  12. Voloshchuk OM, Luchyk TV, Kopylchuk GP. Indicators of immunoreactivity in rats under conditions of different nutrition regimen. Biol Tvarin. 2021; 23(1): 12-7. CrossRef
  13. Lomako V.V. Blood leukocyte indices in male rats of different ages. Advan Gerontol. 2020; 10(2): 135-41. CrossRef
  14. Hogan SP, Rosenberg HF, Moqbel R, Phipps S, Foster PS, Lacy P, Kay AB, Rothenberg ME. Eosinophils: biological properties and role in health and disease. Clin Exp Allergy. 2008; 38(5): 709-50. CrossRef PubMed
  15. Long H, Liao W, Wang L, Lu Q. A player and coordinator: the versatile roles of eosinophils in the immune system. Transfus Med Hemother. 2016; 43(2), 96-108. CrossRef PubMed PubMedCentral
  16. Lomako VV, Pirozhenko LM. Effect of sryopreserved human sord blood on leukocyte indices in rats. Probl Cryobiol Cryomed. 2021; 32(1):058-062. CrossRef

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