THE HYPOTENSIVE ACTIVITY OF DIFERENT FRACTIONS OF THE EXTRACTS FROM TISSUE CULTURE BIOMASS OF RAUWOLFIA SERPENTINA BENTH
O.S. Khromov1, N.V. Dobrelia1, O.V. Parshikov1, I.I. Konvalyuk2, O.A. Bieda2,3, L.P. Mozhylevska2, S.M. Yarmoluk2, A.I. Soloviev1 , V.A. Kunakh2
- State Institution «Institute of Pharmacology and Toxicology of the National Academy of Medical Sciences of Ukraine», Kyiv, Ukraine
- Institute of Molecular Biology and Genetics of the NAS of Ukraine
- Scientific service company “OTAVA” LLC, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz69.04.045
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Abstract
Tissue culture of Rauwolfia serpentina is an alternative source
of more than 20 indole alkaloids that have antiarrhythmic,
hypotensive, psychotropic, and anti-inflammatory effects. The
paper was aimed at determining the hypotensive activity of
five fractions (containing different combinations of alkaloids)
of the extracts from the biomass of R. serpentina tissue culture
(K-27M line). To achieve this aim the following methods were
used: in vitro plant tissue culture, HPLC analysis of indole
alkaloids, assessment of the effects of fractions on the smooth
muscle of the isolated rat thoracic aorta in vitro and on the
blood pressure after intravenous administration in vivo. The
five fractions of the extract from cell biomass of R. serpentina
(K-27M line) were isolated from dried or live biomass and
differed in both qualitative and quantitative compositions. The
direction of action and the magnitude of the effect were found
to depend on the percentage composition of alkaloids in the
fraction. It was shown that fraction 1 containing ajmaline and
acetylajmaline (total alkaloids content of 2.2%) and fraction
2 containing ajmaline, raucaffricine, and acetylajmaline (total
alkaloids content of 6.4%) exerted vasoconstrictor effects on
thoracic aorta muscles. Fraction 4 containing vomilenine,
acetylajmaline, methylajmalicine, ajmalicine, and rauffloridine
(total alkaloids content of 65%) and fraction 5 containing
mainly acetylajmaline with lesser amounts of vomilenine,
methylajmalicine, and ajmalicine (total alkaloids content
of 47.4%) were found to have a relaxing activity in vitro.
Intravenous administration of fractions 4 and 5 resulted in a
short-time (15–30 s) hypotensia, with blood pressure drop by
25% to 30%. All the 5 fractions of the extracts from Rauwolfia
serpentina K-27M line demonstrated either vasoconstrictor or
relaxing effects depending on the alkaloids type and content.
Keywords:
tissue culture; Rauwolfia serpentine; indole alkaloids; vascular tonus; average blood pressure (ABP)
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