EFFECT OF NEURAMINIDASE INHIBITION ON VARIATION OF FIELD EXTRACELLULAR POTENTIALS IN RAT HIPPOCAMPAL NETWORK
A.V. Savotchenko1, E.V. Isaeva2, D.S. Isaev1
- Bogomoletz Institute of Physiology of National Academy of Science of Ukraine, Kyiv, Ukraine
- Medical College of Wisconsin, Milwaukee, USA
DOI: https://doi.org/10.15407/fz69.04.040
Abstract
Neuraminidase (NEU) is the key enzyme that cleaves negatively charged sialic acids of the cell surface. The results of
numerous studies suggest that an imbalance in NEU metabolism due to various pathological conditions significantly
affects nervous activity. Thus, it was shown that downregulation of NEU leads to such morphological changes as accumulation of sialic acid residues in the extracellular space
and synaptogenesis. However, the functional ability of newly
formed synapses remains unclear. Using the specific blocker
2-deoxy-2,3-dehydro-N-acetylneuraminic acid (NADNA), we
examined the effect of NEU inhibition on synaptic efficacy in
the hippocampal CA3-CA1 neuronal network. The significant
increase in the amplitude of synaptic responses due to NEU
blockage was accompanied by the decrease in their variability,
which indicates the enhanced functional excitatory synaptic
connectivity in the hippocampal CA3-to-CA1 network.
Keywords:
neuraminidase blocker; polysialic acids; hippocampus; synaptogenesis; synaptic efficacy.
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