Українська English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2023; 69(4): 40-44

EFFECT OF NEURAMINIDASE INHIBITION ON VARIATION OF FIELD EXTRACELLULAR POTENTIALS IN RAT HIPPOCAMPAL NETWORK

A.V. Savotchenko1, E.V. Isaeva2, D.S. Isaev1

  1. Bogomoletz Institute of Physiology of National Academy of Science of Ukraine, Kyiv, Ukraine
  2. Medical College of Wisconsin, Milwaukee, USA
DOI: https://doi.org/10.15407/fz69.04.040


Abstract

Neuraminidase (NEU) is the key enzyme that cleaves negatively charged sialic acids of the cell surface. The results of numerous studies suggest that an imbalance in NEU metabolism due to various pathological conditions significantly affects nervous activity. Thus, it was shown that downregulation of NEU leads to such morphological changes as accumulation of sialic acid residues in the extracellular space and synaptogenesis. However, the functional ability of newly formed synapses remains unclear. Using the specific blocker 2-deoxy-2,3-dehydro-N-acetylneuraminic acid (NADNA), we examined the effect of NEU inhibition on synaptic efficacy in the hippocampal CA3-CA1 neuronal network. The significant increase in the amplitude of synaptic responses due to NEU blockage was accompanied by the decrease in their variability, which indicates the enhanced functional excitatory synaptic connectivity in the hippocampal CA3-to-CA1 network.

Keywords: neuraminidase blocker; polysialic acids; hippocampus; synaptogenesis; synaptic efficacy.

Full text: (PDF, in Ukrainian)

References

  1. Rougon G. Structure, metabolism and cell biology of polysialic acids. Eur J Cell Biol. 1993; 61(2): 197-207.
  2. James W, Agnew W. ?-(2-8)-polysialic acid immunoreactivity in voltage-sensitive sodium channel of eel electric organ. Proc Roy Soc Lond B Biol Sci. 1989; 1287: 233-45. CrossRef PubMed
  3. Zuber C, Lackie P, Catterall W, Roth J. Polysialic acid is associated with sodium channels and the neural cell adhesion molecule N-CAM in adult rat brain. J Biol Chem. 1992; 267: 9965-71. CrossRef PubMed
  4. Isaev D, Isaeva E, Shatskih T, Zhao Q, Smits N, Shworak N, Khazipov R, Holmes G. Role of extracellular sialic acid in regulation of neuronal and network excitability in the rat hippocampus. J Neurosci. 2007; 27(43): 11587-94. CrossRef PubMed PubMedCentral
  5. Zhang Y, Hartmann H, Satin J. Glycosylation influences voltage-dependent gating of cardiac and skeletal muscle sodium channels. J Membr Biol. 1999; 171: 195-207. CrossRef PubMed
  6. Bonfanti L, Theodosis D. Polysialic acid and activitydependent synapse remodeling. Cell Adh Migr. 2009; 3(1): 43-50. CrossRef PubMed PubMedCentral
  7. Gascon E, Vutskits L, Kiss J. Polysialic acid-neural cell adhesion molecule in brain plasticity: from synapses to integration of new neurons. Brain Res Rev. 2007; 56(1): 101-18. CrossRef PubMed
  8. Ednie A, Bennett E. Modulation of voltage-gated ion channels by sialylation. Compr Physiol. 2012; 2(2): 1269-301. CrossRef PubMed
  9. Rutishauser U, Landmesser L. Polysialic acid in the vertebrate nervous system: a promoter of plasticity in cellcell interactions. Trends Neurosci. 1996; 19(10): 422-7. CrossRef PubMed
  10. Savrasova A, Lushnikova I, Isaeva E, Skibo G, Isaev D, Kostyuk P. The effect of neuraminidase blocker on gabazine-induced seizures in rat hippocampus. Fiziol Zh. 2010; 56(4): 14-8. CrossRef
  11. Venero C, Herrero A, Touyarot K, Cambon K, LopezFernandez M, Berezin V, Bock E, Sandi C. Hippocampal up-regulation of NCAM expression and polysialylation plays a key role on spatial memory. Eur J Neurosci. 2006; 23(6): 1585-95. CrossRef PubMed
  12. Isaeva E, Lushnikova I, Savrasova A, Skibo G, Holmes G, Isaev D. Blockade of endogenous neuraminidase leads to an increase of neuronal excitability and activitydependent synaptogenesis in the rat hippocampus. Eur J Neurosci. 2010; 32(11): 1889-96. CrossRef PubMed
  13. Usami A, Sasaki T, Satoh N, Akiba T, Yokoshima S, Fukuyama T, Yamatsugu K, Kanai M, Shibasaki M, Matsuki N, Ikegaya Y. Oseltamivir enhances hippocampal network synchronization. J Pharmacol Sci. 2008; 106(4): 659-62. CrossRef PubMed
  14. Papatheodoropoulos C, Kostopoulos G. Decreased ability of rat temporal hippocampal CA1 region to produce longterm potentiation. Neurosci Lett. 2000; 279(3): 177-80. CrossRef PubMed
  15. Savotchenko A, Romanov A, Isaev D, Maximyuk O, Holmes G, Isaeva E. Neuraminidase inhibition primes short-term depression and suppresses longterm potentiation of synaptic transmission in the rat hippocampus. Neural Plast. 2015; 2015:1-10. CrossRef PubMed PubMedCentral
  16. Noristani H, Meadows R, Olabarria M, Verkhratsky A, Rodr??guez J. Increased hippocampal CA1 density of serotonergic terminals in a triple transgenic mouse model of Alzheimer's disease: an ultrastructural study. Cell Death Dis. 2011; 2(9): 1-11. CrossRef PubMed PubMedCentral
  17. Ruan Y, Han X, Shi Z, Lei Z, Xu Z. Remodeling of synapses in the CA1 area of the hippocampus after transient global ischemia. Neurosci. 2012; 218: 268-77. CrossRef PubMed
  18. Kerchner G, Nicoll R. Silent synapses and the emergence of a postsynaptic mechanism for LTP. Nat Rev Neurosci. 2008; 9(11): 813-25. CrossRef PubMed PubMedCentral
  19. Vaithianathan T, Matthias K, Bahr B, Schachner M, Suppiramaniam V, Dityatev A, Steinhauser C. Neural cell adhesion molecule-associated polysialic acid potentiates ??-amino-3-hydroxy-5-methylisoxazole-4-propionic acid receptor currents. J Biol Chem. 2004; 279(46): 47975-84. CrossRef PubMed
© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2024.