MORPHO-FUNCTIONAL STATUS OF GOBLET CELLS AND CHANGES IN THE COLONIC MICROBIOTA DURING OBESITY IN RATS
I.M. Vareniuk, T. M. Serhiichuk, M.E. Dzerzhynsky
Taras Shevchenko National University of Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz69.03.039
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Abstract
Changes between the main types of mucins in the goblet cells
and colonic microbiota in obese rats were studied. Paraffin
histological slides of the colon of control and obese rats
were stained with periodic Schiff (to visualize all mucins in
the goblet cells), alcian blue with pH 2.5 (to visualize acidic
mucins), aldehyde fuschin (to visualize sulphomucins), alcian
blue and periodic Schiff (to distinguish between neutral and
acidic mucins), alcian blue and aldehyde fuschin (to distinguish
between sialo- and sulphomucins). Also, the composition of
microbiota in feces was determined on selective and differential media. It has been shown, that obesity is accompanied by
hypoplasia and hypertrophy of goblet cells in the crypts of the
colon. The decrease in the total number of goblet cells in obese
animals occurs due to cells producing mixed mucins with a
predominance of acidic mucins, as well as cells producing
sulphomucins or mixed sulphomucins and sialomucins. As
result, the percentage of goblet cells producing neutral, mixed
neutral-acidic or sialomucins increases. The percentage of
cells producing acidic-neutral mucins, sulfomucins or mixed
sialo- and sulfomucins decreases. At the same time, the number
of Bifidobacterium and lactose-fermenting E. coli (sucrolytic
bacteria) decreases in feces. The number of mannitol-negative
staphylococci (transient microbiota) increases. The number
of Clostridium, Staphylococcus aureus and yeast-like fungi
Candida does not change.
Keywords:
obesity; colon; goblet cells; mucins; microbiota
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