PREDICTORS OF HEPATOCYTES CYTOLYSIS OF INSULIN RESISTANT RATS UNDER CONDITIONS OF PRE- AND POSTNATAL IODINE DEFICIENCY
V.B. Stetseviat1, N.M. Voronych-Semchenko1, M.M. Bahrii2, T.V. Huranych1
- Ivano-Frankivsk National Medical University, Ukraine
- Institute of the Pathology and Cytology, University Clinic Ruppin-Brandenburg, Federal State Brandenburg, Germany
DOI: https://doi.org/10.15407/fz69.03.010

Abstract
Carbohydrate metabolism markers, thyroid profile indexes,
transaminase activity and structural features of liver were
studied, taking into account sexual dimorphism, in rats which
were keeping on a high-fructose diet under conditions of
adequate iodine supply and pre- and postnatal iodine deficiency.
It was found that under fructose loading of animals with iodine
deficiency the risk of insulin resistance (IR) development
increases (HOMA-IR index in insulin-resistant male rats under
conditions of pre- and postnatal iodine deficiency increases
respectively by 22 and 13% compared to the data in animals
that received a high-carbohydrate diet and adequate iodine
supply). It is important, that in males and females with IR, the
secretion of free triiodothyronine decreased by 36 and 40%,
thyroid-stimulating hormone – by 28 and 34%, respectively,
compared to the data of intact animals. The development of
IR was accompanied by the activation of aminotransferases in
blood serum (more in males). In particular, in male rats with
IR under conditions of pre- and postnatal iodine deficiency,
the activity of aspartate aminotransferase increased by 2.7 and
2.4 times, alanine aminotransferase – by 3.9 and 2.7 times,
respectively, which led to a decrease of the de-Ritis index
(more significantly against the background of prenatal iodine
deficiency – by 25%) in relation to the data in animals that were
on a standard diet. The activity of aminotransferases in rats
with IR under conditions of iodine deprivation was higher than
in animals with isolated iodine deficiency – by 11-56% and IR
– by 13-47 %. In animals with IR, the presence of binucleated
hepatocytes with a diffuse decrease in the number of glycogen
granules in them and accumulation of glycoproteins in the
wall of liver vessels were found. The foci of fatty dystrophy
and pronounced zonal character of dystrophic processes in
hepatocytes were identified in females, diffuse dystrophy
prevailed in males. In the cytoplasm of hepatocytes of animals
with IR under conditions of prenatal iodine deficiency, the
lightening and the granularity, single granules of glycogen
were observed. Under these conditions, the average perimeter
(by 74%) and area (by three times) of hepatocytes, the average
area of nucleus (by 3.1 times), the average perimeter (by 50%)
and area (by 66%) of the nucleolus were increased compared
to the control values. The structural changes were consistent
with an increase of the transaminase activity. Thus, fructose loading and the development of IR acts as a trigger for cytolysis
of hepatocytes, which increases under conditions of iodine
deficiency, especially prenatal.
Keywords:
hepatobiliary system; aminotransferases; insulin resistance; pre- and postnatal iodine deficiency
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