GOLD NANOPARTICLES EFFECTS ON THE MORPHOFUNCTIONAL STATE OF THE HYPOTHALAMUS AND ADRENAL CORTEX OF RATS WITH OBESITY
A.S. Pustovalov1, M.G. Matviienko2, G.Ya. Grodzyuk3, N.S. Andryushina3, M.E. Dzerzhynsky1
- Taras Shevchenko National University of Kyiv, Educational
and Scientific Centre “Institute of Biology and Medicine”, Ukraine
- State Ecological Academy of Postgraduate Education and
Management of the Ministry of Environmental Protection
and Natural Resources of Ukraine, Kyiv, Ukraine
- L.V. Pisarzhevskii Institute of Physical Chemistry of the
National Academy of Sciences of Ukraine, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz69.02.052
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Abstract
The aim of our study was to compare the effects of a gold salt
(sodium tetrachloroaurate) and gold nanoparticles colloidal
solution on the morphofunctional state of the hypothalamus
and adrenal glands of 6-month-old male rats with obesity,
which was induced using a diet-induced model of metabolic
disorders. The rats, modeled for obesity, were divided into
three groups that received a NaCl solution, gold salt solution
and colloidal solution of gold nanoparticles for 10 days. Rats
of the control group received a standard diet. After the end of
the experiment, the hypothalamic nuclei and adrenal glands,
prepared according to the appropriate histological methods,
were collected from the animals. In the paraventricular
nucleus of the hypothalamus, a small-cell area of neurons
was examined. In the adrenal glands, cells of three zones
of the cortex were examined: glomerular, fascicular, and
reticular. The results of the study showed that obesity led to
the increase of functional activity in the nuclei of neurons in
the small-cell region of the paraventricular nucleus of the rat
hypothalamus. Functional activity increased maximally in the
cells of the glomerular zone of the adrenal cortex, moderately
increased in the fascicular zone, and no significant changes
were recorded in the reticular zone. It was shown that the gold
salt caused a decrease in the functional activity of neurons of
the paraventricular nucleus of the hypothalamus in obese rats.
In the glomerular zone of the adrenal cortex, the functional
activity of cells significantly decreased; in the fascicular zone,
the parameters of cell nuclei decreased to a greater extent;
in the reticular zone, there were almost no changes. The
introduction of gold nanoparticles colloidal solution caused
the intensification of functional activity of the paraventricular
hypothalamic nucleus cells in obese rats. In the glomerular
zone of the adrenal cortex, cell parameters decreased; in the
fascicular zone, cytoplasmic parameters decreased, but nuclear
parameters did not change; in the reticular zone, measured
values of nuclei increased and cytoplasmic parameters
remained unchanged. In general, the administration of gold
nanoparticles colloidal solution produced more pronounced
effects than gold salt solution on cells of the paraventricular
nucleus of the hypothalamus and the adrenal cortex in obese
rats.
Keywords:
rat; paraventricular hypothalamic nucleus; adrenal gland; gold nanoparticles; sodium tetrachloroaurate; obesity.
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