CORRECTIVE EFFECT OF GERMANIUM CITRATE ON THE REPRODUCTIVE FUNCTION OF FEMALE OLD MICE
O.A. Kondratska, N.G. Grushka, S.I. Pavlovich, R.I. Yanchii
O.O. Bogomoletz Institute of Physiology National Academy
of Sciences of Ukraine, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz69.02.037
Abstract
Fertility decreases with a woman’s age, which is primarily
associated with a deterioration in the quality and a decrease in
the number of oocytes. Oxidative stress is the most likely factor
influencing the decline in oocyte competence. The study of the
effect of drugs with antioxidant properties can be a successful
strategy for preventive intervention to improve the reproductive potential of women, which is of great medical and social
importance. The aim of this work was to evaluate the effect
of Ge citrate, obtained by electropulse nanotechnology, on the
viability of ovarian granulosa cells, the state of the pro- and
antioxidant systems, and the metabolic activity of neutrophils
in female old mice. The studies were carried out on mature
females of the Albino line (young - at the age of 6-8 weeks,
weighing 20-22 g; old - at the age of 8-9 months, weighing
30-34 g). The results showed that in female aged mice, there
was a decrease in viability and an increase in necrotic and
apoptotic death of ovarian granulosa cells (GC). An increase
in the content of the reactive products of 2-thiobarbituric acid
in the liver tissue was revealed. With regard to antioxidant
protection, a decrease in the level of ceruloplasmin in the blood
serum was observed, however, the level of reduced glutathione
in the liver homogenate was slightly increased. There was also
an increase in the metabolic activity of neutrophils. The use of
Ge citrate had a cytoprotective effect on the viability of GC,
reducing their necrotic and apoptotic death. In addition, the
effect of Ge citrate was accompanied by a significant decrease
in lipid peroxidation, regulation of antioxidant protection, and
a decrease in the functional activity of inflammatory effector
cells, which was confirmed by a decrease in the activation of
acid-independent and acid-independent metabolism of neutrophils in the peripheral blood of old mice. In addition, the
effect of Ge citrate was accompanied by a significant decrease
in lipid peroxidation, the regulation of antioxidant protection,
and a decrease in the functional activity of inflammatory effector cells, which was confirmed by a decrease in the activation
of oxygen-independent and oxygen-dependent metabolism
of peripheral blood neutrophils in old mice. Thus, the results
obtained in old female mice showed a positive effect of Ge
citrate in preventing the development of oxidative stress, which
is considered the main mechanism underlying ovarian aging.
Keywords:
reproductive aging; germanium citrate; granulosa cell viability; TBA-reactive products; reduced glutathione; ceruloplasmin.
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