Українська English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2023; 69(2): 37-43


CORRECTIVE EFFECT OF GERMANIUM CITRATE ON THE REPRODUCTIVE FUNCTION OF FEMALE OLD MICE

O.A. Kondratska, N.G. Grushka, S.I. Pavlovich, R.I. Yanchii

    O.O. Bogomoletz Institute of Physiology National Academy of Sciences of Ukraine, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz69.02.037

Abstract

Fertility decreases with a woman’s age, which is primarily associated with a deterioration in the quality and a decrease in the number of oocytes. Oxidative stress is the most likely factor influencing the decline in oocyte competence. The study of the effect of drugs with antioxidant properties can be a successful strategy for preventive intervention to improve the reproductive potential of women, which is of great medical and social importance. The aim of this work was to evaluate the effect of Ge citrate, obtained by electropulse nanotechnology, on the viability of ovarian granulosa cells, the state of the pro- and antioxidant systems, and the metabolic activity of neutrophils in female old mice. The studies were carried out on mature females of the Albino line (young - at the age of 6-8 weeks, weighing 20-22 g; old - at the age of 8-9 months, weighing 30-34 g). The results showed that in female aged mice, there was a decrease in viability and an increase in necrotic and apoptotic death of ovarian granulosa cells (GC). An increase in the content of the reactive products of 2-thiobarbituric acid in the liver tissue was revealed. With regard to antioxidant protection, a decrease in the level of ceruloplasmin in the blood serum was observed, however, the level of reduced glutathione in the liver homogenate was slightly increased. There was also an increase in the metabolic activity of neutrophils. The use of Ge citrate had a cytoprotective effect on the viability of GC, reducing their necrotic and apoptotic death. In addition, the effect of Ge citrate was accompanied by a significant decrease in lipid peroxidation, regulation of antioxidant protection, and a decrease in the functional activity of inflammatory effector cells, which was confirmed by a decrease in the activation of acid-independent and acid-independent metabolism of neutrophils in the peripheral blood of old mice. In addition, the effect of Ge citrate was accompanied by a significant decrease in lipid peroxidation, the regulation of antioxidant protection, and a decrease in the functional activity of inflammatory effector cells, which was confirmed by a decrease in the activation of oxygen-independent and oxygen-dependent metabolism of peripheral blood neutrophils in old mice. Thus, the results obtained in old female mice showed a positive effect of Ge citrate in preventing the development of oxidative stress, which is considered the main mechanism underlying ovarian aging.

Keywords: reproductive aging; germanium citrate; granulosa cell viability; TBA-reactive products; reduced glutathione; ceruloplasmin.

References

  1. Bertoldo MJ, Listijono DR, Ho WJ, Riepsamen AH, Goss DM, Richani D, et al. NAD+ Repletion rescues female fertility during reproductive aging. Cell Rep. 2020;30(6):1670-81. CrossRef PubMed PubMedCentral
  2. Tatone C, Di Emidio G, Vitti M, Di Carlo M, Santini S Jr, D'Alessandro AM, et al. Sirtuin functions in female fertility: possible role in oxidative stress and aging. Oxid Med Cell Long. 2015;2015:659687. CrossRef PubMed PubMedCentral
  3. Peters AE, Mihalas BP, Bromfield EG, Roman SD, Nixon B, et al. Autophagy in female fertility: a role in oxidative stress and aging. Antioxid Redox Signal. 2020;32(8):550-68. CrossRef PubMed
  4. Yong W, Ma H, Na M, Gao T, Zhang Y, Hao L, et al. Roles of melatonin in the field of reproductive medicine. Biomed Pharmacother. 2021;144:112001. CrossRef PubMed
  5. Agarwal A, Gupta S, Sharma RK. Role of oxidative stress in female reproduction. Reprod Biol Endocrinol. 2005;3:28. CrossRef PubMed PubMedCentral
  6. Akila VP, Harishchandra H, D'souza V, D'souza B. Age related changes in lipid peroxidation and antioxidants in elderly people. Ind J Clin Biochem. 2007;22(1):131-4. CrossRef PubMed PubMedCentral
  7. Kosinov MV, Kaplunenko VH. [Pat. of Ukraine No. 38391. 2009. IPC (2006): C07C 51/41, C07F 5/00, C07F 15/00, C07C 53/126 (2008.01), C07C 53/10 (2008.01), A23L 1/00, B82B 3/00. Method for metal carboxylates obtaining «Nanotechnology of obtaining metal carboxylates»]. Publ. 12.01.2009;Bull. N 1:5. [Ukrainian].
  8. Dolaychuk OP, Fedoruk RS, Kropyvka SJ. Physiological reactivity and antioxidant defense system of the animal organism induced by germanium, chromium, and selenium "Nanoaquacitrates". Agricult Sci Pract. 2015;2:50-5. CrossRef
  9. Fedoruk RS, Khrabko MI, Tsap MM, Martsynko OE. Growth, development and reproductive function of female rats and their offspring viability at the conditions of the watering of different doses of citrate germanium. Bìol Tvarin. 2016;18(3):97-106. CrossRef
  10. Fedoruk RS, Tesarivska UI, Khrabko MI, Tsap MM, Dolaychuk OP, Kropyvka SI. Haematological and biochemical parameters of the F2 rats organism in a period of prolonged watering of nano-Ge citrate. Bìol Tvarin. 2017;19(3):115-21. CrossRef
  11. Wrede CE, Dickson LM, Lingohr MK, Briaud I, Rhodes CJ. Protein kinase B/Akt prevents fatty acid-induced apoptosis in pancreatic β-cells (INS-1). J Biol Chem. 2002;277:49676-84. CrossRef PubMed
  12. Devasagayam TP, Boloor KK, Ramasarma T. Methods for estimating lipid peroxidation: an analysis of merits and demerits. Ind J Biochem Biophys. 2003;40(5):300-8.
  13. Giustarini D, Fanti P, Matteucci E, Rossi R. Micro-method for the determination of glutathione in human blood. J Chromatogr B: Analyt Technol Biomed Life Sci. 2014;964:191-4. CrossRef PubMed PubMedCentral
  14. Ravin HA. An improved colorimetric enzymatic assay of ceruloplasmin. J Lab Clin Med. 1961;58:161-8.
  15. Metcalf JA, Gallin JI, Nauseef WM, Root RK. Laboratory manual of neutrophil function. Raven Press. 1985;100.
  16. Nagalievska M, Sabadashka M, Hachkova H, Sybirna N. Galega officinalis extract regulate the diabetes mellitus related violations of proliferation, functions and apoptosis of leukocytes. BMC Complement Altern Med. 2018;18(1):4. CrossRef PubMed PubMedCentral
  17. Reznikov OH, Polumbryk OM, Balion YH, Polumbryk MO. Pro- and antioxidant systems and pathological processes in humans. Visn Nat Akad Nauk Ukrainy. 2014;10:17-29 [Ukrainian]. Material nadiishov do redaktsii 31.01.2023 CrossRef
  18. Gonchar OO, Karaban IM, Karasevich NV, Bratus LV, Mankovska IM. Cerebrolysin administration counteracts elevated oxidative stress in blood of patients with Parkinson's disease. Fiziol Zh. 2022;68(4):20-7. CrossRef
  19. Babayev E, Duncan FE. Age-associated changes in cumulus cells and follicular fluid: the local oocyte microenvironment as a determinant of gamete quality. Biol Reprod. 2022;106(2):351-65. CrossRef PubMed PubMedCentral
  20. Debbarh H, Louanjli N, Aboulmaouahib S, Jamil M, Ahbbas L, Kaarouch I, et al. Antioxidant activities and lipid peroxidation status in human follicular fluid: agedependent change. Zygote. 2021;29(6):490-94. CrossRef PubMed
  21. Ghoneum M, Abdulmalek S, Pan D. Reversal of age-associated oxidative stress in mice by PFT, a novel kefir product. Int J Immunopathol Pharmacol. 2020;34:2058738420950149. CrossRef PubMed PubMedCentral
  22. Sapey E, Greenwood H, Walton G, Mann E, Love A, Aaronson N, et al. Phosphoinositide 3-kinase inhibition restores neutrophil accuracy in the elderly: toward targeted treatments for immunosenescence. Blood. 2014;123(2):239-48. CrossRef PubMed PubMedCentral
  23. Messerer DAC, Schmidt H, Frick M, Huber-Lang M. Ion and water transport in neutrophil granulocytes and its impairment during sepsis. Int J Mol Sci. 2021;22(4):1699. CrossRef PubMed PubMedCentral
  24. De Maeyer RPH, Chambers ES. The impact of ageing on monocytes and macrophages. Immunol Lett. 2021 Feb;230:1-10. CrossRef PubMed
  25. Kramer PA, Prichard L, Chacko B, Ravi S, Overton ET, Heath SL, et al. Inhibition of the lymphocyte metabolic switch by the oxidative burst of human neutrophils. Clin Sci (Lond). 2015;129(6):489-504. CrossRef PubMed PubMedCentral
  26. Teissier T, Boulanger E, Cox LS. Interconnections between inflammageing and immunosenescence during ageing. Cells. 2022;11(3):359. CrossRef PubMed PubMedCentral

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2023.