EFFECT OF NF-ΚB AND NRF2 TRANSCRIPTION FACTOR MODULATORS ON INDICATORS OF OXIDATIVE– NITROSATIVE STRESS IN SKELETAL MUSCLES OF RATS UNDER CHRONIC HYPOMELATONINEMIA AND CARBOHYDRATE-LIPID DIET
Yu.D. Frenkel’1, V.S. Cherno1, V.O. Kostenko2
- Petro Mohyla Black Sea National University, Mykolayiv,
Ukraine
- Poltava State Medical University, Ukraine
DOI: https://doi.org/10.15407/fz69.02.011
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Abstract
This article describes the effect of NF-κB inhibitor ammonium
pyrrolidine dithiocarbamate and Nrf2 inducer dimethylfumarate
on indicators of oxidative-nitrosative stress in skeletal muscles
of rats with chronic hypomelatoninemia, induced by roundthe-clock illumination with an intensity of 1500 lux for 30
days, against the background of a carbohydrate-lipid diet (20%
fructose solution and appropriate food) for 60 days. The study
demonstrated that the administration of ammonium pyrrolidine
dithiocarbamate and dimethylfumarate under the experimental
conditions impedes the development of hypomelatoninemia,
this was accompanied by a two-fold increase in the blood
serum melatonin concentration compared to the control
group. Moreover, the administration of ammonium pyrrolidine
dithiocarbamate and dimethylfumarate significantly reduces
the production of superoxide anion radical by electron
transport chains of mitochondria (by 47.9 and 51.3%) and
sarcoplasmic reticulum (by 48.6 and 52.0%) in the homogenate
of femoral muscles, the total activity of NO synthase (by 37.2
and 36.2%) and its inducible isoform (by 41.1 and 40.0%),
the concentration of peroxynitrites of alkaline and alkaline
earth metals (by 37.2 and 41.0 %), while the activity of
constitutive NO-synthases (by 2.9 and 2.8 times) and their
conjugation index (by 5.2 and 5.4 times) increases compared
to the respective control values. We suggest that modulators
of redox-sensitive transcription factors NF-κB and Nrf2 under
conditions of chronic hypomelatoninemia and the exposure to
the carbohydrate-lipid diet are effective means to restrain the
development of oxidative-nitrosative stress in skeletal muscles.
Keywords:
hypomelatoninemia; round-the-clock lighting; carbohydrate-lipid diet; melatonin; oxidative–nitrosative stress; transcription factors NF-κB and Nrf2; skeletal muscles.
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