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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2023; 69(1): 25-34

INHIBITORY ACTION OF THE GENERAL ANESTHETIC KETAMINE ON INTRACELLULAR CALCIUM TRANSIENTS AND SMOOTH MUSCLE CONTRACTIONS OF THE MOUSE SMALL INTESTINE

M.I. Melnyk1,2, D.O. Dryn1, D.O. Dziuba3, A.V. Zholos2

  1. O.O. Bogomoletz Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine
  2. ESC “Institute of Biology and Medicine”, Taras Shevchenko National University of Kyiv, Ukraine
  3. Shupyk National Healthcare University of Ukraine, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz69.01.025


Abstract

The mechanisms of the negative consequences of general anaesthetics action on the nervous system have been studied in detail, but regarding smooth muscle function, such issues have not yet been sufficiently addressed. In this study, we investigated the effect of the general intravenous anaesthetic ketamine on the level of intracellular calcium in isolated ileum myocytes and the contractile activity of smooth muscle strips of the mouse small intestine. The concentration of intracellular calcium in cells was measured using the Ca2+-sensitive fluorescent dye Fura-2, and tensiometry was used to record the contractile activity of smooth muscles. It was shown that ketamine at a concentration of 100 µM significantly, by 40%, suppressed carbachol-induced contractile reactions of the ileum. The inhibitory effect correlated with the suppression of the intracellular calcium responses to carbachol in isolated smooth muscle cells after the addition of ketamine to the extracellular solution, which was by 65% on average. These results contribute to our better understanding of the possible membrane and intracellular mechanisms of the development of post-surgical intestinal motility disorders.

Keywords: smooth muscles; small intestine; general anesthesia; ketamine; calcium signaling; transient receptor potential channels; contraction recordings; TRPC4 channels agonist and antagonist.

Full text: (PDF, in Ukrainian)

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