THE ROLE OF CELL ENERGY STATUS IN THE PROTECTION OF POSTHYPERTONIC HEMOLYSIS BY AMPHIPHILIC SUBSTANCES
O.Е. Nipot, N.A. Yershova, O.O. Shapkina, N.M. Shpakova, S.S. Yershov, N.V. Orlova
Institute for Problems of Cryobiology and Cryomedicine NAS Ukraine, Kharkiv, Ukraine
DOI: https://doi.org/10.15407/fz68.06.068
Abstract
The effect of ATP depletion on the protection of rabbit eryth-
rocytes by amphiphilic substances in posthypertonic shock
conditions was studied. For this, the level of hemolysis in
posthypertonic shock of native and ATP-depleted erythrocytes
in the presence of amphiphilic substances was determined. It
was shown that ATP depletion reduces posthypertonic cell
damage by 15-17% both at 0°С and at 37°С. It was found that
amphiphilic compounds are able to protect rabbit erythrocytes
in conditions of posthypertonic shock, but only at a temperature
of 0°С. In conditions of ATP-depletion of cells, only anionic
sodium decyl sulfate and nonionic decyl-β,D-glucopyranoside
retain their protective effect in posthypertonic shock condi-
tions, whereas cationic chlorpromazine and trifluoroperazine
lose this ability. This can be explained based on the features
of the incorporation of amphiphiles into the erythrocyte
membrane. All studied amphiphilic compounds increase
the level of damage in both control and ATP-depleted cells
under conditions of posthypertonic shock at a temperature of
37°C. Probably, easier intercalation and increased fluidity of
the membrane at this temperature destabilize the bilayer and
reduce the protective effect of amphiphilic compounds.
Keywords:
rabbit erythrocytes; posthypertonic shock; he- molysis; amphiphilic compounds; ATP; metabolic depletion.
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