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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2022; 68(6): 12-17

Microelement imbalance in the rat uterus after short-term heavy metals exposure

K. Sikora1, Y. Lyndina1, W.A. Awuah1, T. Abdul-Rahman1, R. Chyzhma1, N. Hyriavenko1, M. Lyndin1,2, V. Sikora1,3, A. Romaniuk1

  1. Sumy State University, Ukraine
  2. University of Duisburg-Essen, Germany
  3. University of Foggia, Italy


Heavy metals (HMs) are distinguishable among various exogenous elements with a comprehensive impact and high adverse environmental consequences. The danger of their spread is accompanied by risks of entry into living organisms, leading to organ accumulation, homeostasis disruption, and the development of various inflammatory and oncological clinical manifestations. Interestingly, the uterus shows an unpredictable reaction to the influence of these chemical elements, with almost all previous studies providing differing results. Therefore, this study investigated the features of chemical element content in the rat uterus after 30 days of HMs exposure and assessed the benefits of vitamin E against HMs impacts. Spectral analysis of uterine tissue of rats (daily HMs exposure) and animals (HMs exposure with vitamin E treatment) showed a significantly higher accumulation of Zn, Cu, Fe, Mn, Pb, and Cr after 30 days of the experiment. Although the uterus samples of rats treated with vitamin E showed a significant accumulation of HMs, it should be noted, that the tendency for their accumulation was less pronounced than in untreated rats. This study showed the accumulation features of some chemical elements in the rat uterus after 30 days of HMs exposure. Therefore, widespread environmental HMs exposure can leads to high absorption levels in uterine tissues. However, increases in HMs levels were significantly attenuated by vitamin E supplementation. Unfortunately, while a positive trend was found for each chemical element, the complete protection and purification effect of vitamin E use against HMs exposure was not observed.

Keywords: uterus; heavy metals; accumulation; rats; heavy metals salts.


  1. Delgado-Iniesta MJ, Marín-Sanleandro P, Díaz-Pereira E, Bautista F, Romero-Muñoz M, Sánchez-Navarro A. Estimation of ecological and human health risks posed by heavy metals in street dust of Madrid city (Spain). Int J Environ Res Publ Health. 2022;19(9):5263. CrossRef PubMed PubMedCentral
  2. Tchounwou PB, Yedjou CG, Patlolla AK, Sutton DJ. Heavy metal toxicity and the environment. Exp Suppl. 2012;101:133-64. CrossRef PubMed PubMedCentral
  3. Sakunkoo P, Thonglua T, Sangkham S, Jirapornkul C, Limmongkon Y, Daduang S, et al. Human health risk assessment of PM2.5-bound heavy metal of anthropogenic sources in the Khon Kaen Province of Northeast Thailand. Heliyon. 2022;8(6):e09572. CrossRef PubMed PubMedCentral
  4. Romaniuk A, Lyndin M, Lundina Y, Sikora V, Hrintsova N, Timakova O, et al. Changes in the hematopoietic system and blood under the influence of heavy metal salts can be reduced with Vitamin E. Turk Patol Derg. 2018;34(1):73-81.
  5. Romaniuk A, Sikora V, Lyndin M, Smiyanov V, Sikora V, Lyndina Y, et al. The features of morphological changes in the urinary bladder under combined effect of heavy metal salts. Interv Med Appl Sci. 2017;9(2):105-11. CrossRef PubMed PubMedCentral
  6. Avilova O, Shyian D, Marakushin D, Erokhina V, Gargin V. Ultrastructural changes in the organs of the immune system under the influence of xenobiotics. Georgian Med News. 2018;(279):132-7.
  7. Li Q, Liu H, Alattar M, Jiang S, Han J, Ma Y, Jiang C. The preferential accumulation of heavy metals in different tis- sues following frequent respiratory exposure to PM2.5 in rats. Sci Rep. 2015;5:16936. Publ 2015 Nov 19. CrossRef PubMed PubMedCentral
  8. Sikora K, Lyndin M, Hyriavenko N, Lyndina Y, Sikora V, Romaniuk A. Morphological features of the rat uterus. Pol Merkur Lekarski. 2021;49(294):420-5.
  9. Rzymski P, Niedzielski P, Rzymski P, Tomczyk K, Kozak L, Poniedziałek B. Metal accumulation in the human uterus varies by pathology and smoking status. Fertil Steril. 2016;105(6):1511-8.e3. CrossRef PubMed
  10. Nasiadek M, Danilewicz M, Sitarek K, Świątkowska E, Daragó A, Stragierowicz J, Kilanowicz A. The ef- fect of repeated cadmium oral exposure on the level of sex hormones, estrous cyclicity, and endometrium morphometry in female rats. Environ Sci Pollut Res Int. 2018;25(28):28025-38. CrossRef PubMed PubMedCentral
  11. Lytvynenko M, Narbutova T, Vasylyev V, Bondarenko A, Gargin V. Morphofunctional changes in endometrium under the influence of chronic alcoholism. Georgian Med News. 2021;(315):160-4.
  12. Avilova O, Marakushin D, Nakonechna O, Gargin V. Microscopic features of the spleen under the influence of laproxides. Georgian Med News. 2018;(Issue):163-7.
  13. Al-Attar AM. Antioxidant effect of vitamin E treatment on some heavy metals-induced renal and testicular injuries in male mice. Saudi J Biol Sci. 2011;18(1):63-72. CrossRef PubMed PubMedCentral
  14. Md Amin NA, Sheikh Abdul Kadir SH, Arshad AH, Abdul Aziz N, Abdul Nasir NA, Ab Latip N. Are vitamin E supplementation beneficial for female gynaecology health and diseases? Molecules. 2022;27(6):1896. CrossRef PubMed PubMedCentral
  15. Romaniuk A, Sikora V, Lundina Y, Lundin M, Hyriavenko N, Sikora V, et al. Effect of heavy metals on the readap- tive processes in the urinary bladder. Bangladesh J Med Sci. 2019;18(1):100-6. CrossRef
  16. Lytvynenko M, Narbutova T, Oliynyk N, Lantukh I, Katsap O, Gargin V. Estrogen and progesterone receptors in en- dometrial, cervical and breast tissues in immunodefficient conditions. Azerbaijan Med J. 2021;(3):70-7.
  17. Bove M, Tucci P, Dimonte S, Trabace L, Schiavone S, Morgese MG. Postnatal antioxidant and anti-inflam- matory treatments prevent early ketamine-induced cortical dysfunctions in adult mice. Front Neurosci. 2020;14:590088. CrossRef PubMed PubMedCentral
  18. Doncova V, Lukacinova A, Benacka R, Nistiar F. Effect of low-dose exposure to toxic heavy metals on the re- productive health of rats a multigenerational study. Folia Veterinaria. 2019;63(1):64-71. CrossRef
  19. Schiavone S, Morgese MG, Bove M, Colia AL, Maffione AB, Tucci P, Trabace L, Cuomo V. Ketamine admin- istration induces early and persistent neurochemical imbalance and altered NADPH oxidase in mice. Prog Neuropsychopharmacol Biol Psychiatr. 2020;96:109750. CrossRef PubMed
  20. Chatterjee A, Chatterji U. Arsenic abrogates the estrogen- signaling pathway in the rat uterus. Reprod Biol Endo- crinol. 2010;8:80. CrossRef PubMed PubMedCentral
  21. Diantini NSE, Soeharto S, Wiyasa WA. The effect of lead acetate administration to the uterine malondialdehyde level and endometrial thickness in albino rats (Rattus novergicus). Med J Indones. 2018;27:150-4. CrossRef
  22. Iskra RY, Fedoruk RS. Chromium, its properties, transformation, and impact on humans. Fiziol Zh. 2022;68(4):89-97. CrossRef
  23. Lukačínová A, Rácz O, Lovásová E, Ništiar F. Effect of lifetime low dose exposure to heavy metals on selected serum proteins of Wistar rats during three subsequent generations. Ecotoxicol Environ Saf. 2011;74(6):1747-55. CrossRef PubMed
  24. Morgese MG, Schiavone S, Bove M, Mhillaj E, Tucci P, Trabace L. Sub-chronic celecoxib prevents soluble beta amyloid-induced depressive-like behavior in rats. J Affect Disord. 2018;238:118-21. CrossRef PubMed
  25. Pustovalov AS, Ratushna KV, Matviienko MG, Grod- zyuk GY, Andryushina NS, Dzerzhynsky ME. Morpho- functional changes of the rat hypothalamo-adrenal system under the influence of silver nanoparticles in obesity. Fiziol Zh. 2022;68(2):74-83. [Ukrainian]. CrossRef
  26. Schiavone S, Morgese MG, Mhillaj E, Bove M, De Giorgi A, Cantatore FP, et al. Chronic psychosocial stress impairs bone homeostasis: A study in the social isolation reared rat. Front Pharmacol. 2016;7:152. CrossRef PubMed PubMedCentral
  27. Beschasnyi SP, Hasiuk OM. The carbon monoxide donor, topiramate, and blockers of aquaporine receptors decrease myocardial ischemia-reperfusion injiry. Fiziol Zh. 2021: 67(5):30-8. [Ukrainian]. CrossRef
  28. Morgese MG, Bove M, Francavilla M, Schiavone S, Dimonte S, Colia AL, et al. Sublingual AKBA exerts antidepressant effects in the Aβ-treated mouse model. Biomolecules. 2021;11(5):686. CrossRef PubMed PubMedCentral
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