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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2022; 68(5): 16-24

Vitamin D alters the transcriptional profile of blood cells in patients with primary hypothyroidism

I.I. Kamyshna1, L.B. Pavlovych2, A.M. Kamyshnyi1

  1. I. Horbachevsky Ternopil National Medical University, Ukraine
  2. Bukovinian State Medical University, Chernivtsi, Ukraine
DOI: https://doi.org/10.15407/fz68.05.016


Abstract

Our work aimed to study the influence of cholecalciferol on neurogenesis, neurotrophins and their receptors pathway-focused genes expression in patients with autoimmune thyroiditis and hypothyroidism. The pathway-specific PCR array (Neurotrophins and Receptors RT2 Profiler PCR Array, “QIAGEN”, Germany) was used to identify and validate the neurogenesis regulatory genes expression in patients with thyroid pathology and control group. The results showed that in patients with autoimmune thyroiditis-caused primary hypothyroidism, the expression of BDNF, FAS, FGF2, HSPB1, IL10, NGF, NGFR, NRG1, NTF3, NTF4, TGFB1, and TP53 were increased after treatment with cholecalciferol and L-thyroxine. The mRNA levels of BAX, GDNF, CD40, FOS, GDNF, GFRA1, IL1B, and STAT3 were significantly decreased in patients with autoimmune thyroiditis-caused primary hypothyroidism after treatment with cholecalciferol and Lthyroxine compared to the patients who received only L-thyroxine. Therefore, the addition of vitamin D to standard therapy alters the transcriptional profile of blood cells in patients with primary hypothyroidism.

Keywords: vitamin D; neurogenesis; mRNA; autoimmune thyroiditis; hypothyroidism.

Full text: (PDF, in English)

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