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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2022; 68(5): 16-24


Vitamin D alters the transcriptional profile of blood cells in patients with primary hypothyroidism

I.I. Kamyshna1, L.B. Pavlovych2, A.M. Kamyshnyi1

  1. I. Horbachevsky Ternopil National Medical University, Ukraine
  2. Bukovinian State Medical University, Chernivtsi, Ukraine
DOI: https://doi.org/10.15407/fz68.05.016

Abstract

Our work aimed to study the influence of cholecalciferol on neurogenesis, neurotrophins and their receptors pathway-focused genes expression in patients with autoimmune thyroiditis and hypothyroidism. The pathway-specific PCR array (Neurotrophins and Receptors RT2 Profiler PCR Array, “QIAGEN”, Germany) was used to identify and validate the neurogenesis regulatory genes expression in patients with thyroid pathology and control group. The results showed that in patients with autoimmune thyroiditis-caused primary hypothyroidism, the expression of BDNF, FAS, FGF2, HSPB1, IL10, NGF, NGFR, NRG1, NTF3, NTF4, TGFB1, and TP53 were increased after treatment with cholecalciferol and L-thyroxine. The mRNA levels of BAX, GDNF, CD40, FOS, GDNF, GFRA1, IL1B, and STAT3 were significantly decreased in patients with autoimmune thyroiditis-caused primary hypothyroidism after treatment with cholecalciferol and Lthyroxine compared to the patients who received only L-thyroxine. Therefore, the addition of vitamin D to standard therapy alters the transcriptional profile of blood cells in patients with primary hypothyroidism.

Keywords: vitamin D; neurogenesis; mRNA; autoimmune thyroiditis; hypothyroidism.

References

  1. Shapira Y, Agmon-Levin N, Shoenfeld Y. Geoepidemiology of autoimmune rheumatic diseases. Nat Rev Rheumatol. 2010 Aug;6(8):468-76. CrossRef PubMed
  2. Bizzaro G, Shoenfeld Y. Vitamin D and autoimmune thyroid diseases: facts and unresolved questions. Immunol Res. 2015 Feb;61(1-2):46-52. CrossRef PubMed
  3. Bivona G, Gambino CM, Lo Sasso B, Scazzone C, Giglio RV, Agnello L, Ciaccio M. Serum Vitamin D as a biomarker in autoimmune, psychiatric and neurodegenerative diseases. Diagnostics (Basel). 2022 Jan 6;12(1):130. CrossRef PubMed PubMedCentral
  4. Al-Amin M, Bradford D, Sullivan RKP, Kurniawan ND, Moon Y, Han SH, Zalesky A, Burne THJ. Vitamin D deficiency is associated with reduced hippocampal volume and disrupted structural connectivity in patients with mild cognitive impairment. Hum Brain Mapp. 2019 Feb 1;40(2):394-406. CrossRef PubMed PubMedCentral
  5. Eyles D, Almeras L, Benech P, Patatian A, Mackay-Sim A, McGrath J, Féron F. Developmental vitamin D deficiency alters the expression of genes encoding mitochondrial, cytoskeletal and synaptic proteins in the adult rat brain. J Steroid Biochem Mol Biol. 2007 Mar;103(3-5):538-45. CrossRef PubMed
  6. Kapoor R, Fanibunda SE, Desouza LA, Guha SK, Vaidya VA. Perspectives on thyroid hormone action in adult neurogenesis. J Neurochem. 2015;133(5),599-616. CrossRef PubMed
  7. Kamyshna I, Pavlovych L, Kamyshnyi A. Association between serum brain-derived neurotrophic factor and 25-OH vitamin D levels with vitamin D receptors gene polymorphism (rs2228570) in patients with autoimmune thyroiditis and hypothyroidism. Open Access Maced J Med Sci [Internet]. 2021 Aug. 31 [cited 2021 Oct. 7];9(A):659-64. CrossRef
  8. Kamyshna I, Pavlovych L, Malyk I, Kamyshnyi A. 25- OH Vitamin D blood serum linkage with VDR gene polymorphism (rs2228570) in thyroid pathology patients in the West-Ukrainian population. J Med Life. 2021;14 (4):549-56. CrossRef PubMed PubMedCentral
  9. Kamyshna I, Pavlovych L, Maslyanko V, Kamyshnyi A. Analysis of the transcriptional activity of genes of neuropeptides and their receptors and their receptors in the blood of patients with thyroid pathology. J Med Life. 2021;14(2):1-7. CrossRef PubMed PubMedCentral
  10. Kamyshna I, Pavlovych L, Sydorchuk L, Malyk I, Kamyshnyi A. BDNF blood serum linkage with BDNF gene polymorphism (rs6265) in thyroid pathology patients in the West-Ukrainian population. Endocr Regul. 2021;55(4):101-11. CrossRef PubMed
  11. Topol IA, Kamyshny AM, Abramov AV, Kolesnik YM. Expression of XBP1 in lymphocytes of the small intestine in rats under chronic social stress and modulation of intestinal microflora composition. Fiziol Zh. 2014;60(2):38-44. CrossRef
  12. Sintzel MB, Rametta M, Reder AT. Vitamin D and multiple sclerosis: A comprehensive review. Neurol Ther. 2018 Jun;7(1):59-85. CrossRef PubMed PubMedCentral
  13. Groves NJ, McGrath JJ, Burne TH. Vitamin D as a neurosteroid affecting the developing and adult brain. Annu Rev Nutr. 2014;34:117-41. CrossRef PubMed
  14. Marini F, Bartoccini E, Cascianelli G, Voccoli V, Baviglia MG, Magni MV, Garcia-Gil M, Albi E. Effect of 1alpha,25-dihydroxyvitamin D3 in embryonic hippocampal cells. Hippocampus. 2010 Jun;20(6):696-705. CrossRef PubMed
  15. Shirazi HA, Rasouli J, Ciric B, Rostami A, Zhang GX. 1,25-Dihydroxyvitamin D3 enhances neural stem cell proliferation and oligodendrocyte differentiation. Exp Mol Pathol. 2015 Apr;98(2):240-5. CrossRef PubMed PubMedCentral
  16. Brown J, Bianco JI, McGrath JJ, Eyles DW. 1,25-dihydroxyvitamin D3 induces nerve growth factor, promotes neurite outgrowth and inhibits mitosis in embryonic rat hippocampal neurons. Neurosci Lett. 2003 Jun 5;343(2):139-43. CrossRef
  17. Bayat M, Kohlmeier KA, Haghani M, Haghighi AB, Khalili A, Bayat G, Hooshmandi E, Shabani M. Cotreatment of vitamin D supplementation with enriched environment improves synaptic plasticity and spatial learning and memory in aged rats. Psychopharmacology (Berl). 2021 Aug;238(8):2297-312. CrossRef PubMed
  18. Molteni R, Fumagalli F, Magnaghi V, Roceri M, Gennarelli M, Racagni G. Modulation of fibroblast growth factor-2 by stress and corticosteroids: romdevelopmen talevents to adult brain plasticity. Brainresearch, Brain Res Rev. 2001;37(1-3), 249-58. CrossRef
  19. Dayan CM, Panicker V. Hypothyroidism and depression. Eur Thyroid J 2(3), 168-79, 2013. CrossRef PubMed PubMedCentral
  20. Kristensen B, Hegedus L, Lundy SK, Brimnes MK, Smith TJ, Nielsen CH. Characterization of regulatory B cells in Graves' disease and Hashimoto's thyroiditis. PLoS One. 2015;10(5):e0127949. CrossRef PubMed PubMedCentral
  21. Gerenova J, Stanilova S. IL-12B and IL-10 gene polymorphisms in the development of Hashimoto's thyroiditis. Int J Immunogenet. 2016 Dec;43(6):397-403. CrossRef PubMed
  22. Sterzl I. D vitamin a imunita [D vitamin and immunity]. Vnitr Lek. 2012 May;58(5):405-10.
  23. Christakos S, Hewison M, Gardner DG, Wagner CL, Sergeev IN, Rutten E, Pittas AG, Boland R, Ferrucci L, Bikle DD. Vitamin D: beyond bone. Ann New York Acad Sci. 2013 May;1287(1):45-58. CrossRef PubMed PubMedCentral
  24. Cui C, Cui J, Jin F, Cui Y, Li R, Jiang X, Tian Y, Wang K, Jiang P, Gao J. Induction of the Vitamin D receptor attenuates autophagy dysfunction-mediated cell death following traumatic brain injury. Cell Physiol Biochem. 2017;42(5):1888-96. CrossRef PubMed
  25. Hwang L, Choi IY, Kim SE, Ko IG, Shin MS, Kim CJ, et al. Dexmedetomidine ameliorates intracerebral hemorrhage-induced memory impairment by inhibiting apoptosis and enhancing brain-derived neurotrophic factor expression in the rat hippocampus. Int J Mol Med. 2013 May;31(5):1047-56. CrossRef PubMed
  26. Chen S, Sims GP, Chen XX, Gu YY, Chen S, Lipsky PE. Modulatory effects of 1,25-dihydroxyvitamin D3 on human B cell differentiation. J Immunol. 2007 Aug 1;179(3):1634-47. CrossRef PubMed

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