PECULIARITIES OF SYSTEMIC PATHOBIOCHEMICAL REACTIONS TO BRAIN ISCHEMIA-REPERFUSION IN RATS WITH DIABETES MELLITUS
О.V. Tkachuk1, S.S. Tkachuk1, М.А. Povar1, S.I. Anokhina1, O.V. Yasinska1, S.N. Vadziuk2
- Bukovinian State Medical University, Chernivtsi, Ukraine
- I.Y.Horbachevsky Ternopil National Medical University,
Ternopil, Ukraine
DOI: https://doi.org/10.15407/fz68.04.040

Abstract
The objective of the research was to study the signs of oxidative
stress, the state of proteolysis, and the proteinase-inhibiting
system of the blood plasma in rats with streptozotocin-induced
diabetes mellitus (60 mg/kg intra-abdominally), complicated
by cerebral ischemia-reperfusion. Levels of products of lipid
peroxide oxidation, protein oxidative modification, nitrogen
oxide metabolites, and activity of the antioxidant protection
enzymes were determined by means of biochemical methods
one hour after completion of the reperfusion period and on
the 12th day. Increased intensity of lipid peroxidation was
found to occur in the blood plasma of rats without diabetes
mellitus in both terms of observation. This was accompanied
by an increase in the activity of all the antioxidant enzymes,
while in animals with diabetes the amount of lipid peroxidation products decreased in the ground of prevailing depression
of all the antioxidant protection enzymes. Irrespective of a
tendency of changes in the amount of POM products with
cerebral ischemia-reperfusion, their content is reliably higher
in animals with diabetes mellitus in both terms of observation
than those in animals without diabetes, which is indicative
of a higher intensity of their oxidation. Without diabetes, the
proteolytic activity of the blood plasma reacts by increasing
the values of the studied parameters against the background
of suppression of the proteinase inhibitory system during the
entire observation period, in rats, with diabetes the parameters
of plasma proteolysis and the state of the proteinase-inhibiting
system remain without changes in both terms of the observation. The data obtained allowed us to state that diabetes
mellitus considerably modifies the response of the systemic
pathobiochemical indices to cerebral ischemia-reperfusion.
Keywords:
diabetes mellitus; cerebral ischemia-reperfusion; pathobiochemical disturbances.
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