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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2022; 68(3): 78-85


R.V. Yanko, O.G. Chaka, S.L. Safonov, A.S. Zinchenko, M.I. Levashov

    O.O. Bogomoletz Institute of Physiology of the National Academy of Sciences of Ukraine, Kyiv, Ukraine


The morphofunctional changes of the liver parenchyma of male Wistar rats after administration of L-tryptophan were investigated. Experimental rats in addition to the standard diet received L-tryptophan at a dose of 80 mg/kg. The duration of the experiment was 28 days. Histological preparations were made from liver tissue according to standard methods. Morphometry was performed on digital images using the computer program “Image J”. The activity of hepatic aminotransferases and albumin concentration were determined in serum by biochemical methods; in liver homogenates determined glucose-6-phosphatase activity and triglyceride concentration; in the suspension of hepatocyte mitochondria determined succinate dehydrogenase activity and protein concentration. Multifrequency bioimpedancemetry was used to assess the biophysical properties of the liver. It was found that the administration of L-tryptophan leads to an increase in the nuclear-cytoplasmic ratio (19%), the number of binuclear hepatocytes (81%), and the relative area of the sinusoid (17%). Aspartate aminotransferase activity decreased in serum of experimental rats and albumin concentration increased. In the homogenate of the liver of these animals, the activity of glucose6-phosphatase and the concentration of triglycerides decreased. Succinate dehydrogenase activity and protein concentration in the hepatocyte mitochondrial suspension increased by 35% and 32%, respectively. The method of multifrequency bioimpedancemetry revealed an increase in the ability of the liver tissue of experimental rats to polarize, namely to increases in reactivity. That is, the administration of L-tryptophan is accompanied by the appearance of morphological, biochemical, and biophysical signs of increased synthetic and regenerative activity of the rat liver parenchyma.

Keywords: tryptophan; liver; rats.


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