MORPHOFUNCTIONAL CHANGES IN THE LIVER PARENCHYMA OF RATS WITH THE ADMINISTRATION OF L-TRYPTOPHAN
R.V. Yanko, O.G. Chaka, S.L. Safonov, A.S. Zinchenko, M.I. Levashov
O.O. Bogomoletz Institute of Physiology of the National Academy of Sciences of Ukraine, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz68.03.078
Abstract
The morphofunctional changes of the liver parenchyma of
male Wistar rats after administration of L-tryptophan were
investigated. Experimental rats in addition to the standard diet
received L-tryptophan at a dose of 80 mg/kg. The duration
of the experiment was 28 days. Histological preparations
were made from liver tissue according to standard methods.
Morphometry was performed on digital images using
the computer program “Image J”. The activity of hepatic
aminotransferases and albumin concentration were determined
in serum by biochemical methods; in liver homogenates
determined glucose-6-phosphatase activity and triglyceride
concentration; in the suspension of hepatocyte mitochondria
determined succinate dehydrogenase activity and protein
concentration. Multifrequency bioimpedancemetry was used
to assess the biophysical properties of the liver. It was found
that the administration of L-tryptophan leads to an increase in
the nuclear-cytoplasmic ratio (19%), the number of binuclear
hepatocytes (81%), and the relative area of the sinusoid (17%).
Aspartate aminotransferase activity decreased in serum of
experimental rats and albumin concentration increased. In the
homogenate of the liver of these animals, the activity of glucose6-phosphatase and the concentration of triglycerides decreased.
Succinate dehydrogenase activity and protein concentration
in the hepatocyte mitochondrial suspension increased by
35% and 32%, respectively. The method of multifrequency
bioimpedancemetry revealed an increase in the ability of the
liver tissue of experimental rats to polarize, namely to increases
in reactivity. That is, the administration of L-tryptophan is
accompanied by the appearance of morphological, biochemical,
and biophysical signs of increased synthetic and regenerative
activity of the rat liver parenchyma.
Keywords:
tryptophan; liver; rats.
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