Effect of potentiated cryopreserved mesenchymal stem cells in intervertebral disc repair
M. Yukhta, N. Volkova, L. Stepanyuk, A. Goltsev
Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of
Ukraine, Kharkiv, Ukraine
The aim of the study was to investigate the effect of cryopreserved mesenchymal stem cells (CrMSCs) from
different tissue (bone marrow, adipose tissue, cartilage) potentiated with some growth and differentiation
factors (TGFβ and bFGF) on intervertebral disc (IVD) cartilage repair using compression model of
degenerative damage. In the group with self-healing histological changes in IVD cartilage tissue were
pronounced. Administration of untreated CrMSCs (regardless of their origin) regenerated IVD structure
up to a moderate degree on the 45th day. After therapy with TGFβ-potentiated cells, the histological
structure of the disc remained similar to untreated CrMSC influence, which according to the integrated
semi-quantitative scale corresponded to moderate degenerative changes. Whereas, the administration of
bFGF-potentiated cells contributed to the restoration of the disc structure up to a mild degree of histological
damages. In the latter case, there was an increase in cellularity of the fibrous ring, regeneration of cracks
and gaps, and restoration of collagen febrile structure in annulus fibrosus on the 45th day after therapy.
At the same time, the average cell density of fibrous rings increased by 1.35, 1.50, and 1.39 times and IVD
height grew by 1.33, 1.36, and 1.37 times for bone marrow-, adipose- and cartilage-derived CrMSC of
potentiated by bFGF respectively compared to untreated analog. Thus, administration of CrMSCs from
all studied sources activates regenerative processes in degenerated intervertebral discs of rats, the rate of
which increases using of bFGF-potentiated cells.
cryopreservation; cultivation; mesenchymal stem cells; b-fibroblast growth factors; transforming growth factor β; intervertebral disc; histological structure.
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