EFFECT OF LAPARATOMY AND LIPOPOLYSACCHARIDE-INDUCED SYSTEMIC INFLAMMATORY RESPONSE ON METABOLIC DISORDERS IN RATS
O.V. Taran, N.V. Solovyova, V.O. Kostenko
Poltava State Medical University, Ukraine
DOI: https://doi.org/10.15407/fz68.03.035
Abstract
This investigation is aimed at studying the effect of abdominal
surgical trauma (laparotomy) on markers of surgical stress and
acute phase response as well as markers of carbohydrate and
lipid metabolism under lipopolysaccharide (LPS) -induced
systemic inflammatory response (SIR). Male Wistar rats were
divided into 4 groups: 1st (control) group included “pseudooperated” animals (the procedure included the administration
of anesthesia, epilation, fixation of animals, compression
of the skin of the abdomen with Mikulicz’s clamp by one
click); the 2nd group included the rats, which were injected
Salmonella typhi (in a dose of 0.4 μg/kg body weight 3 times
during the 1st week and once a week for the next 7 weeks)
before performing the “false operation”; the 3rd group was
made up of the rats after laparotomy; and the 4th group
involved the rats after laparotomy performed under LPSinduced SIR. The markers were assessed in 7 days following
the “pseudo-operation” or laparotomy. The results obtained
have demonstrated the combined effect of laparotomy and
LPS-induced SIR was accompanied by a significant increase
in the marker of surgical stress, the concentration of cortisol in
blood plasma, which significantly exceeded the values of the
groups 2 and 3 – by 61.8 and 25.1%, respectively. However, the
content of acute-phase protein ceruloplasmin, an acute phase
reactant, in the serum remained at the level of the 2nd group.
Under these conditions, the concentration of very low-density
lipoprotein cholesterol and triglycerides significantly exceeded
the relevant values in the 2nd and 3rd groups. The combined
effect of surgical trauma and LPS-induced SIR considerably
reduced the activity of constitutive isoforms of NO-synthase,
which was significantly lower, by 41.7%, than the value in
the group 2, and by 41.7% lower than in the group 3. At the
same time, the total activity of this enzyme and the activity
of its inducible isoform were consistent with the values of
the 2nd group. This was accompanied by the development
of decompensated lipid peroxidation (with a considerable
decrease in the blood antioxidant potential).
Keywords:
surgical trauma; laparotomy; lipopolysaccharideinduced systemic inflammatory response; carbohydrate and lipid metabolism; NO-synthase activity; lipid peroxidation
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