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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2022; 68(3): 35-43


EFFECT OF LAPARATOMY AND LIPOPOLYSACCHARIDE-INDUCED SYSTEMIC INFLAMMATORY RESPONSE ON METABOLIC DISORDERS IN RATS

O.V. Taran, N.V. Solovyova, V.O. Kostenko

    Poltava State Medical University, Ukraine
DOI: https://doi.org/10.15407/fz68.03.035

Abstract

This investigation is aimed at studying the effect of abdominal surgical trauma (laparotomy) on markers of surgical stress and acute phase response as well as markers of carbohydrate and lipid metabolism under lipopolysaccharide (LPS) -induced systemic inflammatory response (SIR). Male Wistar rats were divided into 4 groups: 1st (control) group included “pseudooperated” animals (the procedure included the administration of anesthesia, epilation, fixation of animals, compression of the skin of the abdomen with Mikulicz’s clamp by one click); the 2nd group included the rats, which were injected Salmonella typhi (in a dose of 0.4 μg/kg body weight 3 times during the 1st week and once a week for the next 7 weeks) before performing the “false operation”; the 3rd group was made up of the rats after laparotomy; and the 4th group involved the rats after laparotomy performed under LPSinduced SIR. The markers were assessed in 7 days following the “pseudo-operation” or laparotomy. The results obtained have demonstrated the combined effect of laparotomy and LPS-induced SIR was accompanied by a significant increase in the marker of surgical stress, the concentration of cortisol in blood plasma, which significantly exceeded the values of the groups 2 and 3 – by 61.8 and 25.1%, respectively. However, the content of acute-phase protein ceruloplasmin, an acute phase reactant, in the serum remained at the level of the 2nd group. Under these conditions, the concentration of very low-density lipoprotein cholesterol and triglycerides significantly exceeded the relevant values in the 2nd and 3rd groups. The combined effect of surgical trauma and LPS-induced SIR considerably reduced the activity of constitutive isoforms of NO-synthase, which was significantly lower, by 41.7%, than the value in the group 2, and by 41.7% lower than in the group 3. At the same time, the total activity of this enzyme and the activity of its inducible isoform were consistent with the values of the 2nd group. This was accompanied by the development of decompensated lipid peroxidation (with a considerable decrease in the blood antioxidant potential).

Keywords: surgical trauma; laparotomy; lipopolysaccharideinduced systemic inflammatory response; carbohydrate and lipid metabolism; NO-synthase activity; lipid peroxidation

References

    1. Dobson GP. Trauma of major surgery: A global problem that is not going away. Int J Surg. 2020 Sep;81:47-54.2. Dikhtenko TH, Levkov AA, Kostenko VO. Impact of L-arginin immobilized on surgical suture material on the oxidation metabolism in the periwound tissues of the operated small bowel. Klin Khir. 2013 Sep;(9):66-9. [Ukrainian]. 3. Smajic J, Tupkovic LR, Husic S, Avdagic SS, Hodzic S, Imamovic S. Systemic inflammatory response syndrome in surgical patients. Med Arch. 2018;72(2):116-9. 4. Dobson GP, Morris JL, Biros E, Davenport LM, Letson HL. Major surgery leads to a proinflammatory phenotype: Differential gene expression following a laparotomy. Ann Med Surg (Lond). 2021 Oct 21;71:102970. 5. Chen Y, Liu S, Leng SX. Chronic Low-grade Inflammatory Phenotype (CLIP) and senescent immune dysregulation. Clin Ther. 2019 Mar;41(3):400-9. 6. Peng C, Li J, Xu G, Jin J, Chen J, Pan S. Significance of preoperative systemic immune-inflammation (SII) in predicting postoperative systemic inflammatory response syndrome after percutaneous nephrolithotomy. Urolithiasis. 2021 Dec;49(6):513-9. 7. Vogel TR, Smith JB, Kruse RL. The association of postoperative glycemic control and lower extremity procedure outcomes. J Vascul Surg. 2017 Oct;66(4):1123-32. 8. He T, Wang Z, Wu Y, Zhang X, Li X, Li J, Du L, Chen J, Lv Q. Lipid changes during the perioperative period in patients with early breast cancer: a realworld retrospective analysis. BMC Surg. 2021 Nov 12;21(1):396. 9. Belozertseva IV, Dravolina OA, Krivov VO, Tur MA, Polushin YuS. Experimental simulation of postoperative cognitive disorders in rats. Vest Anest Rean. 2016;13(5):37-49. [Russian]. 10. Yelins’ka AM, Shvaykovs’ka OO, Kostenko VO. Epigallocatechin-3-gallate prevents disruption of connective tissue in periodontium and salivary glands of rats during systemic inflammation. Wiad Lek. 2018;71(4):869-73. 11. Tu E, Pearlmutter P, Tiangco M, Derose G, Begdache L, Koh A. Comparison of colorimetric analyses to determine cortisol in human sweat. ACS Omega. 2020;5(14):8211-8. 12. Kaidashev IP, editor. Methods of clinical and experimental research in medicine. Poltava. 2003. [Ukrainian]. 13. Akimov O Ye, Kostenko VO. Functioning of nitric oxide cycle in gastric mucosa of rats under excessive combined intake of sodium nitrate and fluoride. Ukr Biochem J. 2016;88(6):70-5. 14. Yelins’ka AM, Akimov OYe, Kostenko VO. Role of AP-1 transcriptional factor in development of oxidative and nitrosative stress in periodontal tissues during systemic inflammatory response. Ukr Biochim J. 2019;91(1):80-5. 15. Kozaeva RS, Klymenko MO, Kostenko VO. Lipopolysaccharide-induced systemic inflammatory response enhances the development of oxidative-nitrosative stress in salivary glands of rats under alcohol damage. Fiziol Zh. 2021;67(6):60-7. [Ukrainian]. 16. Manou-Stathopoulou V, Korbonits M, Ackland GL. Redefining the perioperative stress response: a narrative review. Br J Anaesth. 2019 Nov;123(5):570-83. 17. Prete A, Yan Q, Al-Tarrah K, Akturk HK, Prokop LJ, Alahdab F, et al. The cortisol stress response induced by surgery: A systematic review and meta-analysis. Clin Endocrinol (Oxf). 2018 Nov;89(5):554-67. 18. Milone M, Desiderio A, Velotti N, Manigrasso M, Vertaldi S, Bracale U, et al. Surgical stress and metabolic response after totally laparoscopic right colectomy. Sci Rep. 2021 May 6;11(1):9652. 19. Nguyen AT, Mandard S, Dray C, Deckert V, Valet P, Besnard P, et al. Lipopolysaccharides-mediated increase in glucose-stimulated insulin secretion: involvement of the GLP-1 pathway. Diabetes. 2014 Feb;63(2):471-82. 20. Mohammad S, Thiemermann C. Role of Metabolic Endotoxemia in Systemic Inflammation and Potential Interventions. Front Immunol. 2021 Jan 11;11:594150. 21. Kaidashev IP. NF-kB Activation as a molecular basis of pathological process by metabolic syndrome. Fiziol Zh. 2012;58(1):93-101. [Ukrainian]. 22. Feingold KR, Grunfeld C. The effect of inflammation and infection on lipids and lipoproteins. In: Feingold KR, Anawalt B, Boyce A, et al., editors. Endotext [Internet]. South Dartmouth (MA): MDText.com, Inc.; 2019. Available from: https://www.ncbi.nlm.nih.gov/books/ NBK326741/ 23. Orekhov AN, Orekhova VA, Nikiforov NG, Myasoedova VA, Grechko AV, Romanenko EB, et al. Monocyte differentiation and macrophage polarization. Vessel Plus. 2019;3:10. 24. Baek N, Sim S, Heo K-S. LPS-stimulated macrophage activation affects endothelial dysfunction. J Bacteriol Virol. 2018 Mar;48(1):23-30. 25. Ekeloef S, Larsen MH, Schou-Pedersen AM, Lykkesfeldt J, Rosenberg J, Gögenür I. Endothelial dysfunction in the early postoperative period after major colon cancer surgery. Br J Anaesth. 2017 Feb;118(2):200-6. 26. Dauphinee SM, Karsan A. Lipopolysaccharide signaling in endothelial cells. Lab Invest. 2006 Jan;86(1):9-22. 27. Morgan MJ, Liu ZG. Crosstalk of reactive oxygen species and NF-κB signaling. Cell Res. 2011 Jan;21(1):103-15.

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