THE STATE OF PERIODONTAL TISSUES IN RATS AGAINST THE BACKGROUND OF THEIR LONG-TERM ALCOHOLIZATION
A.A. Кotvytska1, K.V. Tykhonovych1, Т.D. Кryvoruchko1, S.M. Berehovyi2, К.S. Neporada1
- Poltava State Medical University, Ukraine
- Taras Shevchenko National University of Kyiv, ECS “Institute of Biology and Medicine”, Ukraine
The aim of our research is to study the condition of periodontal
tissues of rats against the background of prolonged alcoholism.
The studies were performed on 19 white nonlinear rats
weighing 180-220 g, which were randomly divided into 2
groups. Rats of the first group served as controls. They were
injected intragastrally with saline for 72 days. The rats of
the second group for 72 days were intragastrically injected
with increasing concentrations of ethanol according to the
following scheme: 1-24 days - 11.8%; 25-48 - 23.6%; 49-
72 days - 37%. In rat soft periodontal tissues, free fucose
content as a monomer of fucoproteins, glycosaminoglycans as
proteoglycan heteropolysaccharides, total proteolytic activity,
total antitryptic activity, TBA-active products, oxidatively
modified proteins and catalase activity was determined. 72-
day alcoholization of rats caused an increase in periodontal
tissue content of TBA-active products by 74.7%, the content
of oxidatively modified proteins by 28.9% and catalase activity
by 33.3%, indicating the activation of free radical processes.
At the same time, the total proteolytic activity in periodontal
tissues decreased by 20.6% against the background of a
decrease in the activity of proteinase inhibitors by 9.6%. Also,
under conditions of long-term administration of ethyl alcohol
to animals an increased depolymerization of fucoproteins and
proteoglycans of periodontal connective tissue was observed,
as evidenced by an increase in free fucose content by 37.5%
and glycosaminoglycans 175.4%. Under conditions of longterm administration of ethyl alcohol to rats, an increased
depolymerization of fucoproteins and proteoglycans of
periodontal connective tissue is observed, which plays a
leading role in the fixation and stability of teeth.
alcohol; periodontal tissues; oxidative stress; proteases; protease inhibitors.
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