THE STATE OF PERIODONTAL TISSUES IN RATS AGAINST THE BACKGROUND OF THEIR LONG-TERM ALCOHOLIZATION
A.A. Кotvytska1, K.V. Tykhonovych1, Т.D. Кryvoruchko1, S.M. Berehovyi2, К.S. Neporada1
- Poltava State Medical University, Ukraine
- Taras Shevchenko National University of Kyiv, ECS “Institute of Biology and Medicine”, Ukraine
DOI: https://doi.org/10.15407/fz68.02.023
Abstract
The aim of our research is to study the condition of periodontal
tissues of rats against the background of prolonged alcoholism.
The studies were performed on 19 white nonlinear rats
weighing 180-220 g, which were randomly divided into 2
groups. Rats of the first group served as controls. They were
injected intragastrally with saline for 72 days. The rats of
the second group for 72 days were intragastrically injected
with increasing concentrations of ethanol according to the
following scheme: 1-24 days - 11.8%; 25-48 - 23.6%; 49-
72 days - 37%. In rat soft periodontal tissues, free fucose
content as a monomer of fucoproteins, glycosaminoglycans as
proteoglycan heteropolysaccharides, total proteolytic activity,
total antitryptic activity, TBA-active products, oxidatively
modified proteins and catalase activity was determined. 72-
day alcoholization of rats caused an increase in periodontal
tissue content of TBA-active products by 74.7%, the content
of oxidatively modified proteins by 28.9% and catalase activity
by 33.3%, indicating the activation of free radical processes.
At the same time, the total proteolytic activity in periodontal
tissues decreased by 20.6% against the background of a
decrease in the activity of proteinase inhibitors by 9.6%. Also,
under conditions of long-term administration of ethyl alcohol
to animals an increased depolymerization of fucoproteins and
proteoglycans of periodontal connective tissue was observed,
as evidenced by an increase in free fucose content by 37.5%
and glycosaminoglycans 175.4%. Under conditions of longterm administration of ethyl alcohol to rats, an increased
depolymerization of fucoproteins and proteoglycans of
periodontal connective tissue is observed, which plays a
leading role in the fixation and stability of teeth.
Keywords:
alcohol; periodontal tissues; oxidative stress; proteases; protease inhibitors.
References
- Fi C, Wo W. Periodontal disease and systemic diseases: an overview on recent progresses. J Biol Regul Homeost Agents. 2021 Jan-Feb;35(Suppl. 1):1-9.
- Dantas AM, Mohn CE, Burdet B, Zubilete MZ, Mandalunis PM, Elverdin JC, Fernández-Solari J. Ethanol consumption enhances periodontal inflammatory markers in rats. Arch Oral Biol. 2012 Sep; 57(9):1211-7.
CrossRef
PubMed
- Frazão DR, Maia CD, Chemelo VD, Monteiro D, Ferreira RD, Bittencourt LO, Balbinot GD, Collares FM, Rösing CK, Martins MD, Lima RR. Ethanol binge drinking exposure affects alveolar bone quality and aggravates bone loss in experimentally-induced periodontitis. PLoS One. 2020 Jul 30;15(7):e0236161.
CrossRef
PubMed PubMedCentral
- Kamoda T, Komatsuzaki A, Ono S, Tanaka S, Yokoi Y. Association between drinking habits and oral symptoms: a cross-sectional study based on japanese national statistical data. Int J Dent. 2020 Dec 8; 2020:1-8.
CrossRef
PubMed PubMedCentral
- Mankovsky BM. Diabetic neuropathy: from head to toe. K.: Vira Project, 2021. [Ukrainan].
- Hammoud N, Jimenez-Shahed J. Chronic neurologic effects of alcohol. Clin Liver Dis. 2019 Feb;23(1):141-55.
CrossRef
PubMed
- Nguyen VA, Le T, Tong M, Mellion M, Gilchrist J, de la Monte SM. Experimental alcohol-related peripheral neuropathy: role of insulin/IGF resistance. Nutrients. 2012 Aug; 4(8):1042-57.
CrossRef
PubMed PubMedCentral
- Dina OA, Barletta J, Chen X, Mutero A, Martin A, Messing RO, Levine JD. Key role for the epsilon isoform of protein kinase c in painful alcoholic neuropathy in the rat. J Neurosci. 2000 Nov 15;20(22):8614-9.
CrossRef
PubMed PubMedCentral
- Santiago S, Ferrer T, Espinosa ML. Neurophysiological studies of thin myelinated (A delta) and unmyelinated (C) fibers: application to peripheral neuropathies. Neurophysiol Clin. 2000 Feb; 30(1):27-42.
CrossRef
- Olukman M, Onal A, Gul Celenk F, Uyanikgil Y, Cavusoglu T, Duzenli N, Ulker S. Treatment with NADPH oxidase inhibitor apocynin alleviates diabetic neuropathic pain in rats. Neural Regen Res. 2018 Sep;13(9):1657-64.
CrossRef
PubMed PubMedCentral
- Mokrik OYa. Optimization of anesthesiological support of surgical interventions in dental patients with different individual-typological features (experimental-clinical study). Dis Doct Med Sci, specialty 14.01.22 - dentistry. Lviv: 2020. [Ukrainan].
- Ugolev AM. Study of the digestive apparatus in humans. L.: Nauka, 1969. [Russian].
- Veremeenko KN. Proteolysis in health and disease. K.: Health, 1988. [Russian].
- Stalnaya ID, Garishvili TG. Method for the determination of malonic dialdehyde using thiobarbituric acid. Modern methods in biochemistry. M.: Medicine, 1977. [Russian].
- Dubinina EE. Oxidative modification of human serum proteins. Method of its determination. Quest Med Chem. 1995;1:24-6. [Russian].
- Korolyuk MA. Ivanova LI, Mayorova IG. Method for determining the activity of catalase. Laboratory. 1988;1:16-9. [Russian].
- Sharaev P N. Method for determination of fucose unbound with proteins. Clin Lab Diagnost. 1997;4:17-8. [Russian].
- Sharaev PN. Method for the determination of glycosaminoglycans in biological fluids. Lab Work. 1987;5:530-2. [Russian].
- Donohue, Jr TM, Thomes PG. Ethanol-induced oxidant stress modulates hepatic autophagy and proteasome activity. Redox Biol. 2014;3:29-39.
CrossRef
PubMed PubMedCentral
- Rukkumani R, Priyanka A, Sankar P, Menon VP. Ferulic acid influences hepatic expression pattern of matrix metalloproteinases during alcohol and PUFA induced toxicity. Eur Rev Med Pharmacol Sci. 2012 Dec;16(15):2147-53.
- Sies H. Role of metabolic H2O2 generation: redox signaling and oxidative stress. J Biol Chem. 2014 Mar 28;289(13):8735-41.
CrossRef
PubMed PubMedCentral
- Demkovych AYe, Bondarenko YuI. Changes of antioxidant potential under the experimental periodontitis development. Fiziol Zh. 2018; 64(3): 43-51.
CrossRef
- Araujo CM, Rocha AC, Araujo BM, Johann AC, Pereira LF, Tanaka OM, Guariza Filho O, Camargo ES. Effect of acute administration of nicotine and ethanol on tooth movement in rats. Braz Oral Res. 2018 Oct 11;32.
CrossRef
- Wang MJ, Kuo JS, LeeWW, Huang HY, ChenWF, Lin SZ. Translational event mediates differential production of tumor necrosis factor-α in hyaluronan-stimulated microglia and macrophages. J Neurochem. 2006 Mar 29;97(3):857-71.
CrossRef
PubMed
- Chen H, Mahaseth M, Zhang Y. Hyaluronic acid as a rescue therapy for trinitrobenzene sulfonic acidinduced colitis through Cox-2 and PGE2 in a Tolllike receptor 4-dependent way. J Zhejiang Univ. 2011 Sep;12(9):712-9.
CrossRef
PubMed PubMedCentral
- Barcia JM, Portolés S, Portolés L, UrdanetaAC, Ausina V, Pérez-Pastor GM, Romero FJ, Villar VM. Does oxidative stress induced by alcohol consumption affect orthodontic treatment outcome? Front Physiol. 2017 Jan 25;8.
CrossRef
PubMed PubMedCentral
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