Українська English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2022; 68(1): 62-68


N.A. Yershova, O.O. Chabanenko, N.М. Shpakova, O.Е. Nipot, N.V. Orlova

    Institute for Problems of Cryobiology and Cryomedicine National Academy of Sciences of Ukraine, Kharkiv? Ukraine


The effects of trifluoroperazine and sodium decyl sulfate on posthypertonic shock of human and rabbit erythrocytes were studied. For this purpose, the level of hemolysis in posthypertonic shock and the percentage of potassium ions released from erythrocytes in dehydration and rehydration solutions in the presence of trifluoroperazine and sodium decyl sulfate were determined. It is shown that the protective effect of amphiphilic compounds is manifested at 0°C, but not at 37°C. There is a decrease in the level of hemolysis in a certain concentration range of each of the studied substances. It was found that human erythrocytes are more sensitive to the protective action of the studied amphiphilic compounds and are characterized by a wider range of protective concentrations. This could be explained by the different lipid composition of the erythrocyte membranes of the studied mammals. Measurement of the leak of potassium ions in dehydration and rehydration media in the presence of amphiphilic compounds suggested that the latter do not affect the permeability of the membrane of mammalian erythrocytes for potassium in posthypertonic shock. Based on the obtained results and literature data, it is assumed that the protective effects of trifluoroperazine and sodium decyl sulfate occur independenly of the formation of temporary defects in permeability for potassium ions, but involve an increase in the cell surface area due to the incorporation of amphiphilic molecules. This results to an increase in the critical hemolytic volume of erythrocytes and reduction in the level of damage during change from hypertonic conditions to isotonic ones.

Keywords: human and rabbit erythrocytes; posthypertonic shock; hemolysis; amphiphilic compounds; potassium ions; membrane permeability.


  1. Muldrew K. The salting-in hypothesis of post-hypertonic lysis. Cryobiology. 2008;57(3):251-56. CrossRef PubMed
  2. Lundbæk JA. Lipid bilayer-mediated regulation of ion channel function by amphiphilic drugs. J Gen Physiol. 2008;131(5):421-29. CrossRef PubMed PubMedCentral
  3. Steinkopf S, Schelderup AK, Gjerde HL, Pfeiffer J, Thoresen S, Gjerde AU, Holmsen H. The psychotropic drug olanzapine (Zyprexa®) increases the area of acid glycerophospholipid monolayers. Biophys Chem. 2008;134(1-2):39-46. CrossRef PubMed
  4. Hägerstrand H, Isomaa B. Amphiphile-induced antihaemolysis is not causally related to shape changes and vesiculation. Chem Biol Interact. 1991;79(3):335-47. CrossRef
  5. Ficarra S, Russo A, Barreca D, Giunta E, Galtieri A, Tellone E. Short-term effects of chlorpromazine on oxidative stress in erythrocyte functionality: activation of metabolism and membrane perturbation. Oxidat Med Cell Longevit. 2016;2016:2394130. CrossRef PubMed PubMedCentral
  6. Yershova NA, Nipot EE, Shpakova NM, Yershov SS, Orlova NV. Effect of trifluoperazine and dodecylβ,D-maltoside on hypertonic stress of mammalian erythrocytes. Probl Cryobiol Cryomed. 2014;24(3):231- CrossRef
  7. [Russian].
  8. Iershov SS, Pysarenko NA, Orlova NV, Shpakova NM. Effect of cationic and anionic amphiphilic compounds on hypertonic cryohemolysis of mammalian red blood cells. Fiziol Zh. 2007;53(6):78-84. [Ukrainian].
  9. Orlova NV, Shpakova NM. Mechanism of protective effect of amphiphilic compounds during hypertonic hemolysis of erythrocytes. Fiziol Zh. 2006;52(5):55-61. [Ukrainian].
  10. Uesono Y, Toh-e A, Kikuchi Y, Araki T, Hachiya T, Watanabe CK, Noguchi K, Terashima I. Local anesthetics and antipsychotic phenothiazines interact nonspecifically with membranes and inhibit hexose transporters in yeast. Genetics. 2016;202(3):997-1012. CrossRef PubMed PubMedCentral
  11. Minetti M, Di Stasi AM. Involvement of erythrocyte skeletal proteins in the modulation of membrane fluidity by phenothiazines. Biochemistry. 1987;26(25):8133-7. CrossRef PubMed
  12. Gorga FR, Lienhard GE. Equilibria and kinetics of ligand binding to the human erythrocyte glucose transporter. Evidence for an alternating conformation model for transport. Biochemistry. 1981;20(18):5108-13. CrossRef PubMed
  13. Raess BU, Vincenzi FF. Calmodulin activation of red blood Cell (Ca2+ + Mg2+)-ATPase and its antagonism by phenothiazines. Mol Pharmacol. 1980;18(2):253-8.
  14. Shpakova NM, Semionova EA, Kovalenko IF, Iershova NA, Orlova NV. Morphological peculiarities of temperature and osmotic response of erythrocytes in presence of chloropromazine. Fiziol Zh. 2017;63(5):62-9. [Ukrainian]. CrossRef
  15. Alvesa I, Stanevab G, Tessierac C, Salgadod GF, Nussac P. The interaction of antipsychotic drugs with lipids and subsequent lipid reorganization investigated using biophysical methods. Biochim Biophys Acta - Biomembr. 2011;1808(8):2009-18. CrossRef PubMed
  16. Wesołowska O, Michalak K, Hendrich AB. Direct visualization of phase separation induced by phenothiazine-type antipsychotic drugs in model lipid membranes. Mol Membrane Biol. 2011;28(2):103-14. CrossRef PubMed
  17. Habibi S, Lee HY, Moncada-Hernandez H, Gooding J, Minericka AR. Impacts of low concentration surfactant on red blood cell dielectrophoretic responses. Biomicrofluidics. 2019;13(5):054101. CrossRef PubMed PubMedCentral
  18. Malheiros SVP, Meirelles NC, de Paula E. Pathways involved in trifluoperazine-, dibucaine- and praziquantelinduced hemolysis. Biophys Chem. 2000;83:89-100. CrossRef
  19. Virtanen J A, Cheng K H, Somerharju P. Phospholipid composition of the mammalian red cell membrane can be rationalized by a superlattice model. Proc Natl Acad Sci USA. 1998;95(9):4964-9. CrossRef PubMed PubMedCentral
  20. Trandum C, Westh P, Jørgensen K, Mouritsen OG. Association of ethanol with lipid membranes containing cholesterol, sphingomyelin and ganglioside: a titration calorimetry study. Biochim Biophys Acta - Biomembr. 1999;1420(1-2):179-88 CrossRef
  21. Verstraeten SL, Deleu M, Janikowska-Sagan M, Claereboudt EJS, Lins L, Tyteca D, Mingeot-Leclercq M-P. The activity of the saponin ginsenoside Rh2 is enhanced by the interaction with membrane sphingomyelin but depressed by cholesterol. Sci Rep. 2019;9:7285. CrossRef PubMed PubMedCentral
  22. Saeedimasine M, Montanino A, Kleiven S, Villa A. Role of lipid composition on the structural and mechanical features of axonal membranes: a molecular simulation study. Sci Rep. 2019;9:8000. CrossRef PubMed PubMedCentral

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2024.