EFFECT OF TRIFLUOROPERAZINE AND SODIUM DECYL SULFATE ON POSTHYPERTENSIVE SHOCK OF HUMAN AND RABBIT ERYTHROCYTES
N.A. Yershova, O.O. Chabanenko, N.М. Shpakova, O.Е. Nipot, N.V. Orlova
Institute for Problems of Cryobiology and Cryomedicine National Academy of Sciences of Ukraine, Kharkiv? Ukraine
DOI: https://doi.org/10.15407/fz68.01.062
Abstract
The effects of trifluoroperazine and sodium decyl sulfate
on posthypertonic shock of human and rabbit erythrocytes
were studied. For this purpose, the level of hemolysis in
posthypertonic shock and the percentage of potassium ions
released from erythrocytes in dehydration and rehydration
solutions in the presence of trifluoroperazine and sodium
decyl sulfate were determined. It is shown that the protective
effect of amphiphilic compounds is manifested at 0°C, but
not at 37°C. There is a decrease in the level of hemolysis in a
certain concentration range of each of the studied substances.
It was found that human erythrocytes are more sensitive to the
protective action of the studied amphiphilic compounds and are
characterized by a wider range of protective concentrations.
This could be explained by the different lipid composition
of the erythrocyte membranes of the studied mammals.
Measurement of the leak of potassium ions in dehydration and
rehydration media in the presence of amphiphilic compounds
suggested that the latter do not affect the permeability of
the membrane of mammalian erythrocytes for potassium
in posthypertonic shock. Based on the obtained results and
literature data, it is assumed that the protective effects of
trifluoroperazine and sodium decyl sulfate occur independenly
of the formation of temporary defects in permeability for
potassium ions, but involve an increase in the cell surface area
due to the incorporation of amphiphilic molecules. This results
to an increase in the critical hemolytic volume of erythrocytes
and reduction in the level of damage during change from
hypertonic conditions to isotonic ones.
Keywords:
human and rabbit erythrocytes; posthypertonic shock; hemolysis; amphiphilic compounds; potassium ions; membrane permeability.
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