EFFICIENCY OF RHYTHMIC COLD EXPOSURES ON THE ACTIVITY OF PROTEINASES AND THEIR INHIBITORS IN RATS WITH ALCOHOL-DEPENDENT HYPERTENSION
L.M. Samokhina1, V.V. Lomako2
- GD L.T. “Malaya National Institute of Therapy of National Academy of Medical Sciences of Ukraine,» Kharkiv, Ukraine
- Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv, Ukraine
The aim of the work is to study the efficiency of rhythmic cold
exposures (RCEs; 5 ± 1°C, frequency 0.1 Hz, 65 min) on the
activities of proteinases, nontrypsin-like proteinases (NTLP),
tripsininhibitory activity (TIA) α-1-proteinase inhibitor
(α-1-PI) and α-2-macroglobulin (α-2-MG) in blood serum,
tissues of the brain and internal organs in male rats with
alcohol-dependent hypertension (ADH) by highly sensitive
(10-9 – 10-10 g) enzymatic methods. ADH was modelled
by chronic (for 10 months) alcoholization of rats by the
«two-bottle» method. It was noted that ADH decreases the
proteinases activity in tissues, maximally in the lungs, kidneys
and heart (by 6, 7 and 10 times, respectively). RCEs promotes
the proteinases activation, it is most pronounced in blood
serum, kidneys and liver (20, 8 and 5 times, respectively), in
intact rats – in the lungs (5 times). ADH decrease the NTLP
activity in the liver by 10 times and less in the kidneys, which
may be due to a violation of protein biosynthesis, and in the
blood serum and brain tissues it increases, in the cerebral
cortex by 10 times. RCEs promotes the NTLP activation, at
ADH by 2-4 times, in the intact rats by 10 or more times. The
ADH decreased the α-2-MG activity, it is most pronounced in
the hypothalamus, lungs, kidneys by 100 times and less in the
heart. RCEs promotes the α-2-MG activation: at ADH below
the control level, in the intact rats – in the brain tissues and
kidneys, which may be due to the participation of syn- and
catatoxical adaptive mechanisms. The α-1-PI activity decreases
at ADH and the background of RCEs, which is associated with
a shift in the balance in the proteinase-proteinase inhibitor
system. Thus, RCEs lead to reverse changes caused by ADH
in rats, promote activation of proteinases, NTLP, α-2-MG,
which is associated with the functioning of regulatory systems
of the body, the development of hormesis, the formation of
high resistance to external and internal stressors, expanding
adaptive capabilities. At the same time, low TIA α-1-IP
promotes activation of proteinases, NTLP.
rhythmic cold exposures; proteinase activity; nontrypsin-like proteinases; α-1-proteinase inhibitor; α-2- macroglobulin; alcohol-dependent hypertension.
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