Українська English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2022; 68(1): 20-27


EFFECT OF EPIGALLOCATECHIN-3- GALLATE AND QUERCETIN ON THE PRODUCTION OF REACTIVE OXYGEN AND NITROGEN SPECIES IN LIVER OF RATS EXPOSED TO ROUND-THE-CLOCK LIGHT AND KEPT ON CARBOHYDRATELIPID DIET

Yu.D. Frenkel’1, V.O. Zyuzin1, V.S. Cherno1, V.O. Kostenko2

  1. Petro Mohyla Black Sea National University, Mykolayiv, Ukraine
  2. Poltava State Medical University, Ukraine
DOI: https://doi.org/10.15407/fz68.01.020


Abstract

The purpose of this study is to investigate the effects of bioflavonoids, epigallocatechin-3-gallate (EGCG) and quercetin, on the production of reactive oxygen and nitrogen species in the liver of rats under round-the-clock light exposure with an intensity of 1500 lux for 30 days while being kept on carbohydrate-lipid diet (20% fructose solution and appropriate food) for 60 days. In the liver tissues, the rate of the superoxide anion production by NADPH-dependent electron transport chains (microsomal monooxygenases and constitutive uncoupled NO synthases) increased by 1.93 times, by the mitochondrial respiratory chain by 1.89 times, and it was doubled by leukocyte NADPH-oxidase. The total activity of NO synthase was increased by 2.35 times, the activity of its inducible isoform increased by 2.57 times, and the concentration of alkali and alkaline earth metals peroxynitrites elevated by 1.68 times. Administration of bioflavonoids-epigallocatechin-3-gallate and quercetinsignificantly restrained the rate of superoxide anion production in the liver tissues by microsomal monooxygenases and NO synthase by 39.1 and 40.1%, by the mitochondrial respiratory chain by 37.2 and 34.4%, by leukocyte NADPH-oxidase by 35.0 and 32.1%, respectively. Epigallocatechin-3-gallate and quercetin inhibited the total activity of NO-synthase by 46.7 and 36.2%, the activity of its inducible isoform by 49.6 and 39,0%, increased the activity of the constitutive isoenzyme NO-synthase by 2.9 times and its coupling index by 4.5 and 4.7 times. Additionally, administration of these bioflavonoids lowered the concentration of peroxynitrites of alkali and alkaline earth metals by 30.5 and 34.3% compared to the respective values obtained in the group of rats, which did not receive the bioflavonoids, but were exposed to light and carbohydrate-lipid-rich diet. We suggest that epigallocatechin3-gallate and quercetin in the above experimental conditions are effective means to restrain the formation of reactive oxygen and nitrogen species in the liver tissue.

Keywords: round-the-clock light; carbohydrate-lipid diet; metabolic syndrome; reactive oxygen and nitrogen species; bioflavonoids; epigallocatechin-3-gallate; quercetin.

References

  1. Kaidashev IP. The role of the molecular clock of circadian rhythms in the pathogenesis of metabolic syndrome. Endokrynologia. 2020;25(2):158-70. [Russian]. CrossRef
  2. Ríos PAZ, Zavala MOQ. Circadian dyssynchrony and its effect on metabolic syndrome parameters in workers. Enfermería Global. 2021;20(2): 603-13.
  3. Rochlani Y, Pothineni NV, Kovelamudi S, Mehta JL. Metabolic syndrome: pathophysiology, management, and modulation by natural compounds. Ther Adv Cardiovascul Dis. 2017;11(8):215-25. CrossRef PubMed PubMedCentral
  4. Mishchenko TV, Gladkih AI, Poltorak VV, Bondarenko LO. Hypopinealism as a factor of metabolic syndrome development. Endokrynologia. 2015; 20(2):494-500. [Ukrainian].
  5. Cardinali DP, Vigo DE. Melatonin, mitochondria, and the metabolic syndrome. Cell Mol Life Sci. 2017 Nov;74(21):3941-54. CrossRef PubMed
  6. Belikova OI, Cherno VS, Frenkel, YuD, Kostenko VO. Influence of chronic hypomelatoninemia on carbohydrate and lipid metabolism of rats kept on "Western pattern diet". Fiziol Zh. 2018;64(3):52-60. [Ukrainian]. CrossRef
  7. Belikova EI, Frenkel YuD, Cherno VS. Influence of exogenous melatonin on free radical processes in rats exposed to light around the clock under modeling of insulin resistance syndrome. Modern Probl Hygiene, Radiat Environment Med (Grodno, Belarus). 2017;(7):35-51. [Russian].
  8. Xu H, Huang L, Zhao J, Chen S, Liu J, Li G. The circadian clock and inflammation: A new insight. Clin Chim Acta. 2021 Jan;512:12-7. CrossRef PubMed
  9. Spengler ML, Kuropatwinski KK, Comas M, Gasparian AV, Fedtsova N, Gleiberman AS, Gitlin II, Artemicheva NM, Deluca KA, Gudkov AV, Antoch MP. Core circadian protein CLOCK is a positive regulator of NF-κBmediated transcription. Proc Natl Acad Sci USA. 2012 Sep 11;109(37):E2457-65. CrossRef PubMed PubMedCentral
  10. Belikova OI, Frenkel, YuD, Cherno VS, Kostenko VO. Influence of nuclear factor κB inhibitor on biochemical markers of insulin resistance syndrome under hypopinealism and high-calorie carbohydrate-lipid diet. Svit Med Biol. 2017;(3):80-2. [Ukrainian]. CrossRef
  11. Frenkel YuD, Cherno VS, Kostenko VO. Effect of ammonium pyrrolidine dithiocarbambate on the formation of reactive oxygen and nitrogen species in liver of rats kept on carbohydrate-lipid diet and exposed to round-the-clock lighting. Aktual Probl Suchasn Med. 2021;21(3):214-8. [Ukrainian]. CrossRef
  12. Chabicovsky M, Prieschl-Grassauer E, Seipelt J, Muster T, Szolar OH, Hebar A, Doblhoff-Dier O. Pre-clinical safety evaluation of pyrrolidine dithiocarbamate. Basic Clin Pharmacol Toxicol. 2010 Sep;107(3):758-67. CrossRef PubMed
  13. Bathaie SZ, Tamanoi F (Eds). Natural products and cancer signaling: isoprenoids, polyphenols and flavonoids. Acad Press. Elsevier; 2014.
  14. Yang H, Landis-Piwowar K, Chan TH, Dou QP. Green tea polyphenols as proteasome inhibitors: implication in chemoprevention. Current Cancer Drug Targets. 2011;11(3):296-306. CrossRef PubMed PubMedCentral
  15. Moybenko AA, editor. Bioflavonoids as organoprotectors (quercetin, corvitin, quertin). Kyiv: Naukova dumka; 2012. [Russian].
  16. Kravtsova-Ivantsiv Y, Ciechanover A. The ubiquitinproteasome system and activation of NF-κB: involvement of the ubiquitin ligase KPC1 in p105 processing and tumor suppression. Mol Cell Oncol. 2015;2(4):e1054552. CrossRef PubMed PubMedCentral
  17. Du X, Yu J, Sun X, Qu S, Zhang H, Hu M, Yang S, Zhou P. Impact of epigallocatechin-3-gallate on expression of nuclear factor erythroid 2-related factor 2 and γ-glutamyl cysteine synthetase genes in oxidative stress-induced mouse renal tubular epithelial cells. Mol Med Rep. 2018:17:7952-8. CrossRef
  18. Sharma A, Parikh M, Shah H, Gandhi T. Modulation of Nrf2 by quercetin in doxorubicin-treated rats. Heliyon. 2020;6(4):e03803. CrossRef PubMed PubMedCentral
  19. Yavtushenko IV, Nazarenko SM, Katrushov OV, Kostenko VO. Quercetin limits the progression of oxidative and nitrosative stress in the rats' tissues after experimental traumatic brain injury. Wiad Lek. 2020;73(10):2127-32. CrossRef PubMed
  20. Yelins'ka AM, Liashenko LI, Kostenko VO. Quercetin potentiates antiradical properties of epigallocatechin-3- gallate in periodontium of rats under systemic and local administration of lipopolisaccharide of Salmonella typhi. Wiad Lek. 2019 Aug 31;72(8):1499-503. CrossRef PubMed
  21. Yelins'ka AM, Kostenko VO. Combined effects of quercetin and modulators of redox sensitive factors on the indicators of systemic inflammatory response, carbohydrate and lipid metabolism in rats exposed to intraperitoneal and intragingival administration of Salmonella typhi lipopolysaccharide. Aktual Probl Suchasn Med. 2020;20(1):13-8. CrossRef
  22. Frenkel' YuD, Belikova OI, Cherno VS, Larycheva OM, Chebotar LD, inventors; Frenkel' YuD, assignee. Method of metabolic syndrome modeling. Ukraine patent UA 122249, Publ. 12. 26. 2017, Bull. No. 24.
  23. Kostenko VO, Tsebrzhins'kii OI. Production of superoxide anion radical and nitric oxide in renal tissues sutured with different surgical suture material. Fiziol Zh. 2000; 46(5):56-62. [Ukrainian].
  24. Akimov O Ye, Kostenko VO. Functioning of nitric oxide cycle in gastric mucosa of rats under excessive combined intake of sodium nitrate and fluoride. Ukr Biochem J. 2016; 88(6):70-5. CrossRef PubMed
  25. Yelins'ka AM, Akimov OYe, Kostenko VO. Role of AP-1 transcriptional factor in development of oxidative and nitrosative stress in periodontal tissues during systemic inflammatory response. Ukr Biochem J. 2019;91(1):80-5. CrossRef
  26. Hwang DH, Kim JA, Lee JY. Mechanisms for the activation of Toll-like receptor 2. 4 by saturated fatty acids and inhibition by docosahexaenoic acid. Eur J Pharmacol. 2016 Aug 15;785:24-35. CrossRef PubMed PubMedCentral
  27. Dekker MJ, Su Q, Baker C, Rutledge AC, Adeli K. Fructose: a highly lipogenic nutrient implicated in insulin resistance, hepatic steatosis, and the metabolic syndrome. Am J Physiol Endocrinol Metab. 2010 Nov;299(5):E685-94. CrossRef PubMed
  28. Morgan MJ, Liu ZG. Crosstalk of reactive oxygen species and NF-κB signaling. Cell Res. 2011 Jan;21(1):103-15. CrossRef PubMed PubMedCentral
  29. Lukyanova LD. Signaling mechanisms of hypoxia. Moscow: Rus Acad Sci; 2019. [Russian].
  30. Zhao RZ, Jiang S, Zhang L, Yu ZB. Mitochondrial electron transport chain, ROS generation and uncoupling. Int J Mol Med. 2019 Jul;44(1):3-15. CrossRef
  31. Oktem G, Uslu S, Vatansever SH, Aktug H, Yurtseven ME, Uysal A. Evaluation of the relationship between inducible nitric oxide synthase (iNOS) activity and effects of melatonin in experimental osteoporosis in the rat. Surg Radiol Anat. 2006 May;28(2):157-62. CrossRef PubMed
  32. Luo S, Lei H, Qin H, Xia Y. Molecular mechanisms of endothelial NO synthase uncoupling. Current Pharm Des. 2014;20(22):3548-53. CrossRef PubMed
  33. Ignarro LJ, Freeman B (Eds). Nitric Oxide: Biology and Pathobiology; 3rd ed. Acad Press; 2017.

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2024.