EFFECT OF EPIGALLOCATECHIN-3- GALLATE AND QUERCETIN ON THE PRODUCTION OF REACTIVE OXYGEN AND NITROGEN SPECIES IN LIVER OF RATS EXPOSED TO ROUND-THE-CLOCK LIGHT AND KEPT ON CARBOHYDRATELIPID DIET
Yu.D. Frenkel’1, V.O. Zyuzin1, V.S. Cherno1, V.O. Kostenko2
- Petro Mohyla Black Sea National University, Mykolayiv, Ukraine
- Poltava State Medical University, Ukraine
DOI: https://doi.org/10.15407/fz68.01.020
Abstract
The purpose of this study is to investigate the effects of
bioflavonoids, epigallocatechin-3-gallate (EGCG) and
quercetin, on the production of reactive oxygen and nitrogen
species in the liver of rats under round-the-clock light
exposure with an intensity of 1500 lux for 30 days while
being kept on carbohydrate-lipid diet (20% fructose solution
and appropriate food) for 60 days. In the liver tissues, the rate
of the superoxide anion production by NADPH-dependent
electron transport chains (microsomal monooxygenases
and constitutive uncoupled NO synthases) increased by
1.93 times, by the mitochondrial respiratory chain by 1.89
times, and it was doubled by leukocyte NADPH-oxidase.
The total activity of NO synthase was increased by 2.35 times, the activity of its inducible isoform increased by 2.57
times, and the concentration of alkali and alkaline earth
metals peroxynitrites elevated by 1.68 times. Administration
of bioflavonoids-epigallocatechin-3-gallate and quercetinsignificantly restrained the rate of superoxide anion production
in the liver tissues by microsomal monooxygenases and NO
synthase by 39.1 and 40.1%, by the mitochondrial respiratory
chain by 37.2 and 34.4%, by leukocyte NADPH-oxidase by
35.0 and 32.1%, respectively. Epigallocatechin-3-gallate and
quercetin inhibited the total activity of NO-synthase by 46.7
and 36.2%, the activity of its inducible isoform by 49.6 and
39,0%, increased the activity of the constitutive isoenzyme
NO-synthase by 2.9 times and its coupling index by 4.5 and
4.7 times. Additionally, administration of these bioflavonoids
lowered the concentration of peroxynitrites of alkali and
alkaline earth metals by 30.5 and 34.3% compared to the
respective values obtained in the group of rats, which did
not receive the bioflavonoids, but were exposed to light and
carbohydrate-lipid-rich diet. We suggest that epigallocatechin3-gallate and quercetin in the above experimental conditions
are effective means to restrain the formation of reactive oxygen
and nitrogen species in the liver tissue.
Keywords:
round-the-clock light; carbohydrate-lipid diet; metabolic syndrome; reactive oxygen and nitrogen species; bioflavonoids; epigallocatechin-3-gallate; quercetin.
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