Українська English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2021; 67(6): 60-67


LIPOPOLYSACCHARIDE-INDUCED SYSTEMIC INFLAMMATORY RESPONSE ENHANCES THE DEVELOPMENT OF OXIDATIVE-NITROSATIVE STRESS IN SALIVARY GLANDS OF RATS UNDER ALCOHOL DAMAGE

R.S. Kozaeva1, M.O. Klymenko1, V.О. Kostenko2

  1. Petro Mohyla Black Sea National University, Mykolayiv, Ukraine
  2. Poltava State Medical University, Ukraine
DOI: https://doi.org/10.15407/fz67.06.060

Abstract

We addressed the role of lipopolysaccharide (LPS)-induced systemic inflammatory response (SIR) in the development of oxidative-nitrosative stress in the salivary glands of rats under the influence of alcohol. Ethanol (40%) at the dose of 24 mg/kg was administered intraperitoneally (ip) twice per day for 14 days. SIR was induced by ip administration of LPS (Salmonella typhi) at the dose 0.4 mg/kg for 1 week followed by a weekly LPS administration for 7 weeks. We found that long-term administration of ethanol in the back- ground of LPS-induced SIR increased the circulating level of proinflammatory markers (TNFa, IL-6) and C-reactive protein and this increase exceeded the respective values when LPS and alcohol were administered separately. Under these conditions, in submandibular salivary glands, the superoxide anion production by mitochondria respiratory chain was increased by 25.9 and 30.5%, by microsomal monooxygenases and NO synthase by 19.0 and 27,1%, by phagocyte NADPH-oxidase by 29.5 and 30.0%. The activity of inducible NO-synthase increased by 15.5 and 83.6%, the concentration of peroxynitrites of alkali and alkali-earth metals elevated by 32.5 and 58, 3%, and S- nitrosothiols raised by 20.2 and 22.7%. These changes were accompanied by a decrease in α-amylase activity and the aquaporin-5 concentration that impairs water and protein excretion by salivary glands. We conclude that adminis- tration of ethanol in the background of LPS-induced SIR results in more pronounced development of oxidative- nitrosative stress in the submandibular salivary glands and more marked dysfunction compared to separate use of LPS and alcohol.

Keywords: lipopolysaccharide-induced systemic inflam- matory response; alcohol; oxidative-nitrosative stress; salivary glands.

References

  1. Global status report on alcohol and health. Geneva: World Health Organization; 2018.
  2. Rajesh E, Sangeetha Priya P, Babu NA, Masthan KMK. A review on effects of alcohol in oral diseases. Ind J Publ Health Res Dev. 2019;10(11):3159-61. CrossRef
  3. Merlo C, Bohl L, Carda C, et al. Parotid sialosis: morphometrical analysis of the glandular parenchyme and stroma among diabetic and alcoholic patients. J Oral Pathol Med. 2010 Jan;39(1):10-5. CrossRef PubMed
  4. Lee KC, Mandel L. Wine and Dental Destruction: Case Report. New York State Dent J. 2017 Apr;83(3):22-5.
  5. Shevchenko KV, Yeroshenko GA, Vilkhova OV, et al. Remodeling of the duct system of the rat submandibular salivary glands in chronic ethanol intoxication. Wiad Lek. 2020;73(1):128-33. CrossRef PubMed
  6. Kim SK, Hong SH, Chung JH, Cho KB. Association Between Alcohol Consumption and Metabolic Syndrome in a Community-Based Cohort of Korean Adults. Med Sci Monit. 2017;23:2104-10. CrossRef PubMed PubMedCentral
  7. Magis DC, Jandrain BJ, Scheen AJ. Alcohol, insulin sensitivity and diabetes. Rev Med Liege. 2003 Jul-Aug; 58(7-8):501-7.
  8. Stafeev IS, Menshikov MY, Tsokolaeva ZI et al. Molecular mechanisms of latent inflammation in metabolic syndrome. Possible role of sirtuins and peroxisome proliferator-activated receptor type γ. Biochemistry (Mosc). 2015 Oct;80(10):1217-26. CrossRef PubMed
  9. Chen Y, Liu S, Leng SX. Chronic Low-grade Inflammatory Phenotype (CLIP) and Senescent Immune Dysregulation. Clin Ther. 2019 Mar;41(3):400-9. CrossRef PubMed
  10. Yelins'ka AM, Shvaykovs'ka OO, Kostenko VO. Influ- ence of ammonium pyrrolidine dithiocarbamate on the production of reactive oxygen and nitrogen species in tissues of periodontium and salivary glands in rats ex- posed to Salmonella typhi lipopolisaccharide. Fiziol Zh. 2018;64(5):63-9. [Ukrainian]. CrossRef
  11. Ceni E, Mello T, Galli A. Pathogenesis of alcoholic liver disease: role of oxidative metabolism. World J Gastroenterol. 2014;20(47):17756-72. CrossRef PubMed PubMedCentral
  12. Yeroshenko GA, Shevchenko KV, Yakushko OS. Morphometric characteristics of rat salivary glands hemomicrovasculature capacity component under normal conditions and in ethanol chronic intoxication. Svit Med Biol. 2018;(3):149-52. CrossRef
  13. Yelins'ka AM, Shvaykovs'ka OO, Kostenko VO. Epigal- locatechin-3-gallate prevents disruption of connective tissue in periodontium and salivary glands of rats during systemic inflammation. Wiad Lek. 2018;71(4):869-73.
  14. Kostenko VO, Tsebrzhins'kii OI. Production of superoxide anion radical and nitric oxide in renal tissues sutured with different surgical suture material. Fiziol Zh. 2000; 46(5):56-62. [Ukrainian].
  15. Akimov O Ye, Kostenko VO. Functioning of nitric oxide cycle in gastric mucosa of rats under excessive combined intake of sodium nitrate and fluoride. Ukr Biochem J. 2016; 88(6):70-5. CrossRef PubMed
  16. Yelins'ka AM, Akimov OYe, Kostenko VO. Role of AP-1 transcriptional factor in development of oxidative and nitrosative stress in periodontal tissues during systemic inflammatory response. Ukr Biochim J. 2019;91(1):80-5. CrossRef Gaston B, Reilly J, Drazen JM et al. Endogenous nitrogen oxides and bronchodilator S-nitrosothiols in human airways. Proc Natl Acad Sci USA. 1993;90(23):10957- CrossRef PubMed PubMedCentral
  17. The effect of alcohol on biological markers associated with the risk of coronary insufficiency: a systematic review and meta-analysis of interventional studies. Líki Ukrayini. 2011;(6):14-21. [Russian].
  18. Sierksma A, van der Gaag M, Kluft C et al. Moderate alcohol consumption reduces plasma C-reactive protein and fibrinogen levels; a randomized, diet-controlled intervention study. Eur J Clin Nutr. 2002; 56:1130-6. CrossRef PubMed
  19. Danovska M, Alexandrova M, Peychinska D, Gencheva I. Alcohol abuse enhances systemic inflammatory response in patients after spontaneous intracerebral haemorrhage. J IMAB. 2010; 16(3):27-31 CrossRef
  20. Ryzhkova OV Alcoholic liver disease. Irkutsk: ISMU; 2021.82 p. [Russian].
  21. Di Meo S, Venditti P. Evolution of the Knowledge of Free Radicals and Other Oxidants. Oxid Med Cell Longev. 2020;2020:9829176. CrossRef PubMed PubMedCentral
  22. Abdelmegeed MA, Ha SK, Choi Y, Akbar M, Song BJ. Role of CYP2E1 in Mitochondrial Dysfunction and Hepatic Injury by Alcohol and Non-Alcoholic Substances. Current Mol Pharmacol. 2017;10(3):207-25. CrossRef PubMed PubMedCentral
  23. Song B.-J., Akbar M, Abdelmegeed M et al. Mitochondrial dysfunction and tissue injury by alcohol, high fat, nonalcoholic substances and pathological conditions through post-translational protein modifications. Redox Biol. 2014; 3C:109-23. CrossRef PubMed PubMedCentral
  24. Vergis N, Khamri W, Beale K et al. Defective monocyte oxidative burst predicts infection in alcoholic hepatitis and is associated with reduced expression of NADPH oxidase. Gut. 2017;66:519-29. CrossRef PubMed PubMedCentral
  25. Akimov OYe, Kostenko VO. Role of NF-κB transcriptional factor activation during chronic fluoride intoxication in development of oxidative-nitrosative stress in rat's gastric mucosa. J Trace Elem Med Biol. 2020;61:126535. CrossRef PubMed
  26. Guijarro-Muñoz I, Compte M, Álvarez-Cienfuegos A et al. Lipopolysaccharide activates Toll-like receptor 4 (TLR4)- mediated NF-κB signaling pathway and proinflammatory response in human pericytes. J Biol Chem. 2014 Jan 24;289(4):2457-68. CrossRef PubMed PubMedCentral
  27. Deng XS, Deitrich RA. Ethanol metabolism and effects: nitric oxide and its interaction. Current Clin Pharmacol. 2007;2(2):145-53. CrossRef PubMed
  28. Zoga M, Tzavellas E, Ioannidis A, et al. Total Nitric Oxide and Inducible Nitric Oxide Synthase in Alcohol- dependent Individuals During Detoxification Therapy. In Vivo.2014; 28(6):1175-9.
  29. Tang Y, Forsyth CB, Farhadi A, et al. Nitric oxide-mediated intestinal injury is required for alcohol-induced gut leakiness and liver damage. Alcoholism, Clin Exp Res. 2009;33:1220-30. CrossRef PubMed PubMedCentral
  30. Wang X, Abdel-Rahman AA. Effect of chronic ethanol administration on hepatic eNOS activity and its association with caveolin-1 and calmodulin in female rats. Am J Physiol Gastrointest Liver Physiol. 2005;289(3):G579-85. CrossRef PubMed
  31. Arias-Salvatierra D, Silbergeld EK, Acosta-Saavedra LC, Calderon-Aranda ES. Role of nitric oxide produced by iNOS through NF-κB pathway in migration of cerebellar granule neurons induced by Lipopolysaccharide. Cell Signal. 2011 Feb;23(2):425-35. CrossRef PubMed
  32. Karbach S, Wenzel P, Waisman A, Munzel T, Daiber A. eNOS uncoupling in cardiovascular diseases - the role of oxidative stress and inflammation. Current Pharm Des. 2014;20(22):3579-94. CrossRef PubMed
  33. Delporte C, Bryla A, Perret J. Aquaporins in Salivary Glands: From Basic Research to Clinical Applications. Int J Mol Sci. 2016;17(2):166. CrossRef PubMed PubMedCentral

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2022.