Українська English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2021; 67(6): 32-39


O.M. Lynnyk1,2, O.I. Osadcha2, H.P. Kozynets1,2, I.R. Yanchiy3, O.O. Shmatova2, G.M. Boiarska2

  1. Shupyk National Healthcare University of Ukraine, Kyiv, Ukraine
  2. Institute of Haematology and Transfusiology of NAMS of Ukraine, Kyiv, Ukraine
  3. Komisarenko Institute of Endocrinology and Metabolism of Ukraine, Kyiv, Ukraine


To study the effect of thermal trauma on the immune response formation, 43 patients aged 16 to 58 with body surface area of burns 20-60% were examined. The neutrophilic granulocytes (NG) and monocytes functional activity, the content of myeloperoxidase and substances with a positive reaction to Schiff’s periodic acid (PAS- stained substances), the cytokines content were determined: interleukin-1β (IL-1β), IL-2, IL-4, IL- 6, tumor necrosis factor-α (TNF-α). Thermal damage caused changes in the immune response, which were characterized by a pro-inflammatory phase in which innate immunity cells (neutrophilic granulocytes, monocytes) acquired high functional activity, producing a higher content of proinflammatory cytokines. On the 2-3rd day after the burn injury in the peripheral blood, the interleukin-1β (IL-1β) content was 133.5 ± 21.1 pg/ml, the tumor necrosis factor α (TNF-α) content was 265 ± 115.5 pg/ml, which exceeded the reference values by 5.1 and 10.9 times, respectively. The content of IL-6 on the 2-3rd day was 85.30 ± 13.10 pg/ml. Also a concomitant syndrome of compensatory anti-inflammatory response developed with increasing production of anti-inflammatory IL-4: on the 2-3rd day after burn injury, its content was 268.5 pg/ml, exceeding the reference values by 8.2 times. The content of anti-inflammatory IL-10 was decreased. This suggests that anti-inflammatory cytokines do not compensate for the high content of pro-inflammatory factors. On the 7th-8th day after the burn injury, there was a tendency to further increase the content of pro-inflammatory cytokines IL- 1β and TNF-α to 148.0 ± 27.0, and 281.2 ± 146.7 pg/ml, respectively, while the content of IL-6 on the 7-8th day was 131.0 ± 11.1 pg/ml, the anti-inflammatory cytokine IL-10 increased slightly, and the content of IL-4 decreased. These changes in the early period of burn disease dynamics, as well as reduced activity of myeloperoxidase and PAS- stained substances NG, point for a functional deficiency of NG, reduced enzymatic activity and cells energy resources decompensation. These changes could clinically lead to SIRS progression and multiorgan dysfunction.

Keywords: thermal trauma; immunological reactivity; systemic inflammatory response syndrome; compensatory anti-inflammatory response; cytokines.


    World Health Organization. Global Health Estimates. 2018. disease/en/
  1. Rock KL, Hearn A, Chen CJ, Shi Y. Natural endog- enous adjuvants. Springer Semin Immunopathol. 2005 Jan;26(3):231-46. CrossRef PubMed
  2. Abreu MT, Arditi M. Innate immunity and toll-like recep- tors: clinical implications of basic science research. J Pediatr. 2004 Apr;144(4):421-9. CrossRef PubMed
  3. Latz E, Xiao TS, Stutz A. Activation and regulation of inflammasome. Nat Rev Immunol. 2013;13:397-411. CrossRef PubMed PubMedCentral
  4. Belozorov AP. Autoinflammatory mechanisms connects with inflammasomapathies, in the pathogenesis of some skin diseases. Dermatol Venerol. 2017;76 (2);8-11.
  5. Contassot E, Beer HD, French LE. Interleukin-1, inflam- masomes, autoinflammation and the skin. Swiss Med Wkly. 2012 May 31;142:w13590. CrossRef PubMed
  6. Yarilin DA. The role of tumor necrosis factor in the regula- tion of the inflammatory response of monocytes and mac- rophages. Immunology. 2014 July-Aug;35(4):195-201.
  7. Gavrilenko TI, Rizhkova NA, Parkhomenko OM, Dov- gan EV, Dovgan NV, Pasichnichenko OM, Babiy SM. Modern views on the role of neutrophils in the immune response. Fiziol Zh. 2021;67(3):75-86. [Ukrainian]. CrossRef
  8. Ruleva NYu, Zvyaghintseva MA, Dughin SF. Myeloper- oxidase: Biological functions and clinical value. Modern Righ Eechnol. 2007;8:11-4.
  9. Moretta A. Natural killer cells and dendritic cells: ren- dezvous in abused tissues. Nat Rev Immunol. 2002 Dec;2(12):957-64. CrossRef PubMed
  10. Boiars'ka HM, Osadcha OI, Kozynets HP. Immunologi- cal reactivity conditions of burned children. Fiziol Zh. 2000;46(6):68-74. [Ukrainian].
  11. Morrison VV, Bozhedomov AYu, Simonyan MA, Morri- son AV. Systemic inflammatory response and cytokine profile at burn injury in dynamics. Saratov J Med Sci Res. 2017 Apr-Jun;13(2);229-32.
  12. Kovalchuk LV, Immunology. Practicum. Moscow, GEO- TAR. 2010.
  13. Kozinets GP, Osadchaya OI, Tsygankov VP, Isaenko NP, Zhernov AA, Boyarskaya AM. Correction of metabolic hypoxia in patients with severe thermal burn injury under septicotoxemia. Klin Khirurgiia. 2012 Dec;838(12):38-42.
  14. Schwacha MG. Macrophages and post-burn immune dysfunction. Burns. 2003 Feb;29(1):1-14. CrossRef
  15. Hesketh M, Sahin KB, West ZE, Murray RZ. Macrophage phenotypes regulate scar formation and chronic wound healing. Int J Mol Sci. 2017 Jul 17;18(7):1545. CrossRef PubMed PubMedCentral
  16. Kovalenko AO. Improvement of surgical treatment of pa- tients with dermal burns through the use of wound dress- ings [dissertation]. Kyiv, 2018. 14.01.03 surgery. 186 p.
  17. Venet F, Foray AP, Villars-Méchin A, Malcus C, Poitevin- Later F, Lepape A, Monneret G. IL-7 restores lympho- cyte functions in septic patients. J Immunol. 2012 Nov 15;189(10):5073-81. CrossRef PubMed
  18. Ni Choileain N, MacConmara M, Zang Y, Murphy TJ, Mannick JA, Lederer JA. Enhanced regulatory T cell activity is an element of the host response to injury. J Immunol. 2006 Jan 1;176(1):225-36. CrossRef PubMed
  19. Artemiev SA, Nazarov IP, Kamzalakova NI, Bulygin GV. Activity of intracellular lymphocyte enzymes in severe burn shock in children. Siberian Med Rev. 2008;50 (2);40-2.
  20. Huang LF, Yao YM, Dong N, Yu Y, He LX, Sheng ZY. Association between regulatory T cell activity and sepsis and outcome of severely burned patients: a prospective, observational study. Crit Care. 2010;14(1):R3. CrossRef PubMed PubMedCentral
  21. Osuka A, Ogura H, Ueyama M, Shimazu T, Lederer JA. Immune response to traumatic injury: harmony and discordance of immune system homeostasis. Acute Med Surg. 2014 Jan 28;1(2):63-9. CrossRef PubMed PubMedCentral
  22. Zaitseva GA, Vershinina OA, Matrokhina OI, Senkina EA, Karpova MV. Cytokine status of donors of blood and its components. Fundament Res. 2011;3:61-5.
  23. Moins-Teisserenc H, Cordeiro DJ, Audigier V, Ressaire Q, Benyamina M, Lambert J, Maki G, Homyrda L, Toubert A, Legrand M. Severe altered immune status after burn injury is associated with bacterial infection and septic shock. Front Immunol. 2021 Mar 2;12:586195. CrossRef PubMed PubMedCentral

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2023.