Українська English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2021; 67(5): 30-38


S.P. Beschasnyi, O.M. Hasiuk

    Kherson State University, Ukraine


We investigated the metabolism of mouse isolated heart under the influence of tricarbonyldichlorothenium (II)- dimer (CORM-2 and 2,3-4,5-bis-O-isopropylidene-βD-fructopyranose sulfamate (topiramate) as potential blockers of aquaporine channel (AQP3) of cardiac myocytes. The results were compared with those obtained from the group receiving anti-AQP3 monoclonal antibodies. A decrease in coronary flow was found during the period preceding ischemia (topiramate did not cause this effect). However, at the end of reperfusion, CORM-2 was responsible for its stabilization. This compound did not affect glucose intake (topiramate increased it only at the end of reperfusion), decreased Ca2+ deposition in cardiac muscle (AQP3-IgG antibodies and topiramate had similar effect), decreased creatinine release, AST (especially at the end of reperfusion). The action of CORM-2 increased the amplitude of the R waveform before ischemia and during reperfusion. At the end of reperfusion the R-wave amplitude decreased. The effect of topiramate caused an increase in amplitude only at the beginning of reperfusion. Administration of CORM-2, topiramate and antibodies resulted in prolongation of the interval before and during ischemia. At the same time, the effect of these drugs and antibodies reduced the development of ischemic damage. The results indicate that the released CO from CORM-2 has effects similar to those of anti-AQP3 antibodies. The action of topiramate had signs of calcium channel blocking.

Keywords: aquaporins; AQP3; gas-transmitter; carbon monoxide donor; CORM-2; isolated heart; topiramat


  1. Sagach VF, Dorofeyeva NA, Drachuk KO. Contribution of hydrogen sulfide to cardio-vascular function restoration in old rats. J Mol Cell Cardiol. 2018;120:35. CrossRef
  2. Panneerselvam L, Raghunath A, Perumal E. Differential expression of myocardial heat shock proteins in rats acutely exposed to fluoride. Cell Stress Chaperones. 2017;22:743-50. CrossRef PubMed PubMedCentral
  3. Ling K, Men F, Wang WC, Zhou YQ, Zhang HW, Ye DW. Carbon monoxide and its controlled release: therapeutic application, detection, and development of carbon monoxide releasing molecules (CORMs) miniperspective. J Med Chem. 2017;61(7):2611-35. CrossRef PubMed
  4. Queiroga CS, Almeida AS, Alves PM, Brenner C, Vieira HL Carbon monoxide prevents hepatic mitochondrial membrane permeabilization. BMC Cell Biol. 2011;12:1-8. CrossRef PubMed PubMedCentral
  5. Beschasnyi S, Hasiuk O. CO-releasing molecule (CORM-2) in the regulation of Ca2+-dependent K+-permeability of erythrocyte. Ukr Zh Med Bìol Sportu. 2020;5(2),166-71. CrossRef
  6. Kukoba TV, Moybenko OO, Kotsjuruba AV. Cardioprotective effect of heme oxigenase-1 induction by hemin on the isolated heart of rat at ischemia/reperfusion. Fiziol Zh. 2003;49(6):14-21. [Ukrainian].
  7. Johnson TE, Wells RJ, Bell A, Nielsen VG, Olver CS. Carbon monoxide releasing molecule enhances coagulation and decreases fibrinolysis in canine plasma exposed to Crotalus viridis venom in vitro and in vivo. Basic Clin Pharmacol Toxicol. 2019;125(4):328-36. CrossRef PubMed
  8. Motterlini R, Foresti R. Biological signaling by carbon monoxide and carbon monoxide-releasing molecules. Am J Physiol Cell Physiol. 2017;312(3):302-13. CrossRef PubMed
  9. Beschasnyi SP, Hasiuk OM. The effect of carbon monoxide's donor CORM-2 on erythrocyte aquaporins. World Med Biol. 2021;17(76):167-73. CrossRef Ikarashi N, Aburada T, Kon R, Sugiyama K. Water control mechanism of byakkokaninjinto and its active components via aquaporins. Traditional Kampo Med. 2019;6(2):57-61. Material nadiishov do redaktsii 17.08.2021 CrossRef
  10. Ma T, Hara M, Sougrat R, Verbavatz JM, Verkman AS. Impaired stratum corneum hydration in mice lacking epidermal water channel aquaporin-3. J Biol Chem. 2002;277:17147-53. CrossRef PubMed
  11. Verkman AS, Anderson MO, Papadopoulos MC. Aquaporins: important but elusive drug targets. Nat Rev Drug Discov. 2014;13(4):259-77. CrossRef PubMed PubMedCentral
  12. Kitchen P, Day RE, Salman MM, Conner MT, Bill RM, Conner AC. Beyond water homeostasis: Diverse functional roles of mammalian aquaporins. Biophys Acta Gen Subj. 2015;1850(12):2410-21. CrossRef PubMed
  13. Bollag WB, Aitkens L, White J, Hyndman KA. Aquaporin-3 in the epidermis: more than skin deep. Am J Physiol Cell Physiol. 2020;318(6):C1144-53 CrossRef PubMed PubMedCentral
  14. Rutkovsky A, Stenslokken KO, Kaljusto M-L, Hafstad A, Larsen T, Vaage J. Degree of phosphorylation of survival kinases in isolated mouse hearts depends on the mode of perfusion. J Mol Cell Cardiol. 2008;44:809. CrossRef
  15. Abir-Awan M, Kitchen P, Salman MM, Conner MT, Conner AC, Conner AC, Bill RM. Inhibitors of mammalian aquaporin water channels. Int J Mol Sci. 2019;20(7):1589. CrossRef PubMed PubMedCentral
  16. Butler TL, Au CG, Yang B, Egan JR, Tan YM, Hardeman EC, Winlaw DS. Cardiac aquaporin expression in humans, rats, and mice. Am J Physiol Heart Circ Physiol. 2006;291(2):H705-13. CrossRef PubMed
  17. Kazory A, Ronco C. Are we barking up the wrong tree? Rise in serum creatinine and heart failure. Blood Purif. 2019;48(3):193-5. CrossRef PubMed
  18. Alyasin A, Momeni HR, Mahdieh M. Aquaporin 3 expression and the potential role of aquaporins in motility and mitochondrial membrane potential in human spermatozoa. Andrologia. 2020;52(6):e13588. CrossRef
  19. Takashi Y, Tomita K, Kuwahara Y, Roudkenar MH, Roushandeh AM, Igarashi K, Sato T. Mitochondrial dysfunction promotes aquaporin expression that controls hydrogen peroxide permeability and ferroptosis. Free Radic Biol Med. 2020;161:60-70. CrossRef PubMed PubMedCentral

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2024.