Effect of caffeine and coffee diets on calcium signalling in rat hippocampal neurons
V.M. Shkryl1, T.G. Turytska2, V.A. Yavorsky1, V.P. Lyashenko2, S.M. Lukashov3, E.A. Lukyanetz1
- Bogomoletz Institute of Physiology National Academy of Sciences of Ukraine, Kyiv, Ukraine
- Dnipro National Oles Honchar University, Dnipro, Ukraine
- Medical center “Headache”, Regional Clinical Hospital N.A. Mechnikov, Dnipro, Ukraine
DOI: https://doi.org/10.15407/fz67.04.037
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Abstract
The effects of long-lasting high concentration coffee and caffeine diets on calcium mobilization in rat
hippocampal neurons were studied. Changes in the basal calcium level in the hippocampal neurons of
control and experimental rats kept on a coffee or caffeine diet were measured. We also recorded the changes
in the Ca2+ transients’ amplitude evoked by membrane depolarization or emptying the Ca2+ depot of the
endoplasmic reticulum (ER) induced by caffeine activator of the ryanodine receptors. In rats on a coffee
or caffeine diet, the basal Ca2+ level was increased by 7.4% and 11%, respectively, compared to control
animals. In these groups, the amplitude of Ca2+ transients increased by 70% and 90%, respectively, of the
basal level in response to the membrane depolarization. In the same groups, the amount of Ca2+ released
from the ER was increased by two and three times, respectively, compared to the control after activation
of ryanodine receptors. We concluded that long-term coffee and caffeine diets in rats cause a significant
disruption of the hippocampal neurons’ endoplasmic reticulum function. The diets evoke an increase in
Ca2+ concentration in the neurons and an excessive release of Ca2+ in response to excitation. The latter
can lead to increased excitability of neurons and their further death from excessive Ca2+ levels.
Keywords:
hippocampal neurons, coffee, caffeine, calcium, ryanodine receptor, endoplasmic reticulum, diet.
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