The aim of the study was to determine the characteristics of the psychophysiological functions of 363 military and 110 civilian pilots by comparative analysis of the results of evaluating their values and the structure of correlation relationships. The analysis of the obtained data was carried out using the well-known methods of variation statistics and correlation analysis. It was shown that among military pilots the best were indicators of neurodynamic functions, attention and reliability of task performance, and among civilians – orientation in space and short-term memory. In the group of military pilots, psychophysiological functions are fairly well correlated with each other and are closely related to most of the personified characteristics, forming 45,5 ± 6,1% of relationships of the maximum number. Among civilian pilots, there are fewer such relationships 24,2 ± 5,3%; there is no correlation between psychophysiological functions and personified characteristics. The regulation of possible negative shifts in psychophysiological functions and the reliability of mission performance in military pilots is carried out primarily as a result of the stabilizing effect of the balance of nervous processes and, to a lesser extent, the strength of nervous processes and orientation in space. Among civilian pilots, such regulation is fairly evenly distributed between the balance of nervous processes, attention, orientation in space, and short-term memory.