Influence of portal hypertension on the state of the hemomicrocirculatory level of rat skin: morphological study
N.R. Grytsevych1, V.V. Vereschaka2
- Higher educational communal institution of the Lviv regional council “Andrei Krupinsky Lviv Medical
Academy”, Lviv, Ukraine
- Educational and Scientific Center “Institute of Biology and Medicine” of Taras Shevchenko National
University of Kyiv; Kyiv, Ukraine
The effect of portal hypertension on the condition of the hemomicrocirculatory tract of the skin male white
nonlinear rats was studied. Hypertension was simulated by applying a ligature to the portal vein according
to the conventional method. After 30, 60 and 90 days, skin biopsies were taken from the anterior abdominal
wall for optical microscopy examination, which was subjected to routine histological processing. In animals
of the control group, which underwent sham surgery, the skin remained unchanged. In rats of the experimental
group after 30 days in the basal layer of the epidermis there were cells with signs of degenerative changes.
The basement membrane of the epidermis had small areas with thinning and thickening, which in both cases
were accompanied by the formation of small and massive leukocyte infiltrates. Its edema was noted, which
extended to the papillary and reticular layer of the dermis. Elastic fibers were unchanged. Only 50% of the
microvessels looked intact, 20% microvessels were narrowed, and 30% had signs of stagnant hyperemia
due to dilatation. After 60 days the epidermis was thinned, in the basal layer the cells with picnotized
and displaced to the periphery nucleus were detected. In all studied layers of epidermis, besides reticular
layer, expansion of arterioles, venules and capillary loops was observed. After 90 days the epidermis was
represented by 3-6 layers of cells, its general refinement was observed due to the reduction of the thickness
of the spinous and granular layers with simultaneous thickening of the horny layer. In the papillary layer of
the dermis, the number of thin-walled blood vessels was increased. The average diameter of the arterioles
of the hypodermis decreased relative to the values on the 60th day. The mean diameter of the arterioles of
the subdermal network on the border of the papillary and reticular layers and capillary loops under the
basal membrane was reduced what can be a sign of sclerotic changes in the microvessels. The venules
diameter remained increased. In arterioles and venules, stasis of the microvessels, edema of their walls,
microaneurysm, and venous sacculation were detected. Thus, we believe that the changes in the microvessels
of the skin, which are observed in portal hypertension, are the result of hemodynamic disturbances in the
tissue, which is a complex chain of hemomicrocirculatory flow to the direct action of hypertension in the
portal vein and is a secondary response to systemic vascular damage.
portal hypertension; morphology; skin; hemomicrocirculation.
- Snegyrev FG. Progress and future of Ukrainian surgery. Ukr Med J. 2019;1(1)-I/II.
- Syiromaha S. Cardiac surgery in Ukraine: problems and prospects of a progressive industry. 2015;amnu.gov.ua› Naukova diyalnyst [Research activities].
- Vereshchaka VV. Pathophysiological mechanismes of the resistantionalreduce of skin capillary in case of patientes. Fiziol Zh. 2000;46(6):116-8. [Ukrainian].
- Kalyuzhnaya LD, Vereshchaka VV. Functional state of the skin in its premature involution. Probl Aging Develop. 2002;6(2):193-8.
- Cherkasov VG, Vereshchaka VV. Ultrastructural changes of facial skin in the process of aging of modern Caucasian type. Rep Morphol. 2005;(2):203-6. [Ukrainian].
- Van der Graaff D, Kwanten WJ, Couturier FJ, Govaerts JS, Verlinden W, Brosius I, D'Hondt M, Driessen A, De Winter BY, De Man JG, Michielsen PP, Francque SM. Severe steatosis induces portal hypertension by systemic arterial hyporeactivity and hepatic vasoconstrictor hyperreactivity in rats. Lab Invest. 2018;98(10):1263-75.
- Schwabl P, Brusilovskaya K, Supper P, Bauer D, Königshofer P, Riedl F, Hayden H, Fuchs CD, Stift J, Oberhuber G, Aschauer S, Bonderman D, Gnad T, Pfeifer A, Uschner FE, Trebicka J, Rohr-Udilova N, Podesser BK, Peck-Radosavljevic M, Trauner M, Reiberger T. The soluble guanylate cyclase stimulator riociguat reduces fibrogenesis and portal pressure in cirrhotic rats. Sci Rep. 2018 Jun 19;8(1):9372.
- Königshofer P, Brusilovskaya K, Schwabl P, Reiberger T. Animal models of portal hypertension. Biochim Biophys Acta Mol Basis Dis. 2019 May1;1865(5):1019-30.
- Sarkisov DS, Perov YuL, editors. Microscopic technique: Manual. Moscow: Medicine; 1996.
- Tsvetkova GM, Mordovtsev VN. Pathomorphological Diagnostics of Skin Diseases: Manual. - Moscow: Medicine; 1986.
- Avtandilov GG. Fundamentals of Quantitative Pathological Anatomy: Study Manual. Moscow: Medicine; 2002.
- Filimonova NB, Fil IO, Mykhailova TS. Statistical analysis of data in accordance with the principles of science of sound medicine. Medicine of Ukrainian Transport. 2004;4:85-93.
- Diskovskyi IS. Morphological peculiarities of the skin and reparation processes under the influence of opioid in experiment. Thesis for a Candidate Degree in Medicine: specialty 14.03.01 - normal anatomy. - Ivano-Frankivsk National Medical University, Ministry of Health of Ukraine, Ivano-Frankivsk, 2016.