Modifications of proteins of membrane-cytoskeleton complex and production of reactive oxygen species in erythrocytes cryopreserved with polyethylene glycol
N.G. Zemlianskykh, L.O. Babiychuk
Institute for Problems of Cryobiology and Cryomedicine, National Academy of Sciences of Ukraine, Kharkov, Ukraine
DOI: https://doi.org/10.15407/fz67.02.044
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Abstract
Protein modifications in the membrane-cytoskeleton complex (MCC) of human erythrocytes, as well as
changes in the intensity of reactive oxygen species (ROS) production upon cell cryopreservation with
polyethylene glycol (PEG) were investigated. The protein profile of ghosts of erythrocytes frozen with PEG
has common features with both the control and cells frozen without cryoprotectant. PEG makes it possible
to restrict the structural rearrangements of the main MCC proteins under the effect of extreme factors and
to restrain the amount of high molecular weight polypeptide complexes induced by the protein-cross-linking
reagent diamide at the control level, in contrast to cells frozen without a cryoprotectant. However, changes
related to the protein peroxiredoxin 2 in ghosts of erythrocytes cryopreserved with PEG are also attributed
to cells frozen without a cryoprotectant that may be associated with the activation of oxidative processes.
This is evidenced by a 10-fold increase in ROS formation in erythrocytes frozen under PEG protection. Thus,
upon cryopreservation of erythrocytes with PEG, certain disorders in MCC proteins may be associated with
increased formation of ROS, which may contribute to the disorganization of the structural components of
MCC and disrupt the stability of cryopreserved cells under physiological conditions.
Keywords:
Erythrocyte; membrane; cytoskeleton; protein; reactive oxygen species; cryopreservation; polyethylene glycol.
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