CYTOCHROMES OF MITOCHONDRIES AND ACTIVITY OF HEME METABOLISM ENZYMES IN THE LIVER UNDER DIFFERENT NUTRIENT REGIMES
G.P. Kopylchuk, Z.-M. I. Grynenkiv, O.M. Voloshchuk
Yuriy Fedkovych Chernivtsi National University, Ukraine
DOI: https://doi.org/10.15407/fz67.02.037
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Abstract
The content of mitochondrial cytochromes and the activity of
key enzymes of heme metabolism in the liver of rats under conditions of different dietary supply of protein and sucrose were
investigated. The quantitative determination of mitochondrial
cytochrome was performed by differential spectrophotometry,
δ-aminolevulinate synthase activity was determined spectrophotometrically taking into account the molar extinction
coefficient of 0.023x10(3) M(-1)sm(-1). Hemoxygenase activity
was determined using the amount of formed bilirubin. It was
found that under conditions of consumption of high-sucrose
diet a significant decrease in the content of all mitochondrial
cytochromes is noted: the content of cytochromes aa3, b and
c1 decreases within 1.2-1.7 times, and content of cytochrome
c decreases in two times. In the case of excessive consumption
of sucrose on the background of alimentary protein deprivation
the content of cytochromes b and c1 in the liver of rats does
not differ statistically from similar indicators of the group of
animals kept on a high-sucrose diet. At the same time, the
content of cytochromes aa3 and c is significantly reduced.
According to the activity of δ-aminolevulinate synthase
under conditions of consumption of a high-sucrose diet, the studied enzymatic activity decreases by about 1.5 times with
a simultaneous increase in the activity of heme oxygenase.
Thus, there is a marked decrease in heme synthesis against
the background of increased catabolism, which explains the
decrease in the content of cytochromes in the mitochondria
of the liver of rats under conditions of excess sucrose in the
diet. The maximum increase in the activity of heme oxygenase
(almost threefold) is observed in animals that were kept on
a high-sugar diet deficient in protein content. Thus, dietary
protein deficiency is a critical factor affecting the heme metabolism in the mitochondria of liver cells. The established
changes in the content of mitochondrial cytochromes and the
activities of key enzymes of heme metabolism in the liver
could be considered as prerequisites for deepening its energy
imbalance in conditions of different supply of sucrose and
protein in diet.
Keywords:
Nutrients; mitochondria; cytochrome; δ-aminolevulinic acid synthase; hemoxygenase.
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