BKCA CHANNELS MEDIATE THE EFFECTS OF DOCOSAHEXAENOIC ACID ON THE RESPIRATION PARAMETERS OF MYOCARDIAL MITOCHONDRIA AT HIGH CALCIUM CONCENTRATIONS
O.S. Panasiuk, А.I. Bondarenko
O.O. Bogomoletz Institute of Physiology, NAS Ukraine, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz66.06.074
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Abstract
Omega-3 polyunsaturated fatty acids (PUFA) provide
protection against myocardial damage in ischemia-reperfusion. However, the mechanisms that provide cardioprotection are
not fully understood. In this study, we investigated the effect
of docosahexaenoic acid (DHA), a member of omega -3
PUFA, on mitochondrial respiration parameters and the role of
mitochondrial calcium-dependent potassium channels of large
conductance (ВКСа) in the implementation of these effects.
Using the patch-clamp method, it was shown that functional
ВКСа channels are expressed in the inner mitochondrial
membrane of cardiac cells and their activity increases with the
addition of DHA. We investigated the role of mitochondrial
ВКСа channels in the regulation of mitochondrial respiratory
processes. In experiments with isolated mitochondria from
rat hearts, we showed that DHA prevented an increase in the
respiratory rate of mitochondria in the V4 state and a decrease
in the respiratory control elicited by addition of 10 μM Ca2+.
Qualitatively the same effect was caused by NS1619, the ВКСа
opener. In the presence of 10 μM Ca2+, the ВКСа channel
inhibitor paxilin (1 μM) prevented the protective effect of
DHA and NS1619 on the parameters of respiratory control.
We conclude that mitochondrial ВКСа channels are involved
in the implementation of the effects of DHA on mitochondrial
respiration.
Keywords:
mitochondria; polyunsaturated fatty acids; potassium channels.
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