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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2002; 48(3): 3-8


Effect of acetylcholineand adenosine triphosphate on membrane potential of the intact endotheliumof rat aorta in aging

M.N. Tkachenko V.V. Yarotsky S.M. Marchenko V.F. Sagach

    A.A. Bogomoletz Institute of Physiology NationalAcademy of Science of Ukraine, Kiev


Abstract

Changes in the electric reactions of intact endothelium of isolated rat aorta were studied in conditions of aging. A value of resting membrane potential of endothelium of old rats (24-26 mo) was significantly greater, than in control group of animals (6 mo). In responses to acetylcholine and ATP the old rats did not show a typical course of reactions, which was peculiar to control animals. Inability of old rats to produce a typical response to acetylcholine and ATP may signify a disturbance of links between endothelial cells, which resulted in functional clusterization of the latter. The data obtained suggest a possible mechanism of decrease in production and release of potent vasodilator – nitric oxide – due to changes that occur in the electric properties of endothelium in aging.

References

  1. Реутов В.П., Сорокина Е.Г., Охотин В.Е., Косицын Н.С. Циклические превращения оксида азота в организме млекопитающих. – М.: Наука, 1997. – 158 с.
  2. Ткаченко М.М. Оксид азоту та судинна регуляція // Журн. АМН України. – 1997. – 3, № 2. –С. 241-254.
  3. Фролькис В.В., Безруков В.В., Кульчицкий О.К.Старение и экспериментальная патология сердечнососудистой системы. – К.: Наук. думка, 1994. – 248 с.
  4. Яроцький В.В., Сагач В.Ф., Марченко С.М.Електричні властивості інтактного ендотелію аорти кроля // Фізіол. журн. – 2001. – 47, № 1. –С. 9-16.
  5. Alvarez de Sotomayor M., Stoclet J.C., Andriantsitohaina R. Effect of ageing on the endotheliumdependent vasorelaxation induced by acetylcholine in rat mesenteric arteries. - In: Endothelium-Deri-ved Hyperpolarizing Factor / Ed. P.M.Vanhoutte. -Harwood Academic Publishers, Amsterdam, 1999. -P. 323-332.
  6. Atkinson J., Tatchum-Talom R., Capdeville-Atkinson C. Reduction of endothelial function with age in the mesenteric arterial bed of the normotensive rat // Br. J. Pharmacol. – 1994. – 111, № 4. –P. 1184-1188.
  7. Cernadas M.R, Sanchez de Miguel L., Garcia-Duran M. et al. Expression of constitutive and inducible nitric oxide synthases in the vascular wall of young and aging rats // Circulat. Res. – 1998. – 83, № 3. –P. 279-286.
  8. Chou T.C., Yen M.N., Li C.Y., Ding Y.A. Alterations of nitric oxide synthase expression with aging and hypertension in rats // Hypertension. – 1998. –31, № 2. – P. 643-648.
  9. 9. Dohi Y., LЬsсher T.F. Aging differentially affects direct and indirect actions of endothelin-1 in perfused mesenteric arteries of the rat // Br. J. Pharmacol. – 1990. – 100, № 4. – P. 889-893.
  10. 10. Egashira K., Inou T., Hirooka Y. et al. Effects of age on endothelium-dependent vasodilation of resistance coronary artery by acetylcholine in humans // Circulat. – 1993. – 88, № 1. – P. 77-81.
  11. Hongo K., Nakagomi T., Kassel N.F. et al. Effects of aging and hypertension on endothelium-dependent vascular relaxation in rat carotid artery //Stroke. – 1988. – 19, № 7. – P. 892-897.
  12. Koga T., Takata Y., Kobayashi K. et al. Ageing supresses endothelium-dependent relaxation and generates contraction mediated by the muscarinic receptors in vascular smooth muscle of normotensive Wistar-Kyoto and spontaneously hypertensive rats // J. Hypertension. – 1988. – 6,Suppl. 4. – P. S243-245.
  13. KЬng C.N.F., LЬscher T.F. Different mechanisms of endothelial dysfunction with aging and hypertension in rat aorta // Hypertension. – 1995. –25, № 2. – P. 194-200.
  14. Marchenko S.M., Sage S.O. Electrical properties of resting and acetylcholine-stimulated endothelium in intact rat aorta // J. Physiol. – 1993. –462, № 3. – P. 735-751
  15. Marchenko S.M., Sage S.O. Mechanism of acetylcholine action on membrane potential of endothelium of intact rat aorta // Amer. J. Physiol. 1994. – 266, № 5. – P. H2388-95.
  16. Matz R.L., Schott C., Stoclet J.C., Andriantsitohaina R. Age-related endothelial dysfunction with respect to nitric oxide, endothelium-derived hyperpolarizing factor and cyclooxygenase products// Physiol. Res. – 2000. – 49, № 1. – P. 11-18.
  17. Moritoki H., Hosoki E., Ishida Y. Age-related decrease in endothelium-dependent dilator response to histamine in rat mesenteric artery // Eur. J.Pharmacol. – 1986. – 126, № 1-2. – P. 61-67.
  18. Nilius B., Viana F., Droogmans G. Ion channels in vascular endothelium // Annu. Rev. Physiol. –8 ISSN 0201-8489 Фізіол. журн., 2002, Т. 48, № 31997. – 59, № 2. – P. 145-170.
  19. 19. Shimizu I., Toda N. Alterations with age of the response to vasodilator agents in isolated mesenteric arteries of the beagle // Br. J. Pharmacol. – 1986. – 89, № 4. – P. 769-778.
  20. 20. Taddei S., Virdis A., Mattei P. et al. Aging and endothelial function in normotensive subjects and patients with essential hypertension // Circulat. –1995. – 91, № 7. – P. 1981-1987.
  21. Tschudi M.R., Barton M., Bersinger N.A. et al. Effect of age on kinetics of nitric oxide release in rat aorta and pulmonary artery // J. Clin. Invest. –1996. – 98, № 4. – P. 899-905.
  22. Usachev Y.M., Marchenko S.M., Sage S.O. Cytosolic calcium concentration in resting and stimulated endothelium of excised intact rat aorta // J. Physiol. – 1995. – 489, № 2. – P. 309-317.
  23. Vane J.R., Botting R.M. Control of the circulation by vasoactive mediators from endothelial cells // J. Lipid Mediat. Cell Signal. – 1994. – 9, № 1. – P. 1-8.

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