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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2020; 66(6): 3-12


EFFECT OF CALCIUM LOAD ON HEART FUNCTION, MPTP OPENING IN SITU AND UCP2/3 MRNA EXPRESSION IN THE HEART OF TRAINED RATS

Yu.V. Goshovska, N.A. Strutynska, V.F. Sagach

    О.О. Bogomoletz Institute of Physiology of NAS of Ukraine, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz66.06.003

Abstract

We have studied the effect of calcium load (1.7 to 15 mmol/l in perfusate) on isolated heart function, mitochondrial factor release (as a marker of mitochondrial permeability transition pore, MPTP), and cardiac uncoupling proteins (UCP2/3) mRNA expression in untrained and trained rats (swimming for 4 weeks). It was found that the improvement in the isolated heart function of trained rats was accompanied by an increase in the expression of UCP3, but not UCP2. A gradual increase of the calcium content in the perfusate led to an increase in contractile function, more pronounced in trained rats. However, 10 mmol/l and higher concentration of calcium led to arrhythmia and drastic decrease in contractility of isolated heart more obvious in untrained rats. Swimming course prevented the calcium-induced release of mitochondrial factor exerting a stabilizing effect on mitochondrial membranes which was, however, diminished by a nitric oxide synthesis blocker (L-NAME). We have found that UCPs genes expression is calcium-sensitive: an increase in UCP3 mRNA at 5 mmol of calcium and a sharp decrease in UCP2/3 expression at 12.5 mmol/l of calcium in perfusate in both trained and untrained rats indicating the participation of UCPs in the regulation of calcium homeostasis. Our data suggest that the calcium load may serve as a test for in situ MPTP titration. Activation of UCPs together with up-regulated nitric oxide may play a protective role against increasing extracellular calcium inhibiting MPTP formation during physical trainings.

Keywords: heart; nitric oxide; calcium load; mitochondrial uncoupling proteins; mitochondrial permeability transition pore; training; swimming.

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