STATE OF NEUROENDOCRINE SYSTEMS DURING ACTIVATION AND INHIBITION OF CENTRAL CHOLINERGIC SYSTEMS IN BRAIN INJURY
S.V. Ziablitsev1, S.O. Khudoley2
- Bogomolets National Medical University, Kyiv, Ukraine
- Medical Center for Modern Addictology “MedicoMente”, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz66.05.064
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Abstract
The aim of the study was to determine the state of the
neuroendocrine systems during the activation and inhibition
of the central cholinergic systems (CChS) in traumatic brain
injury (TBI) and their effect on mortality and neurological
deficit. TBI was applied by the standard method with the
free fall of metal weight on the fixed animal head. 161
white male Wistar rats were divided into three groups:
in the 1st group, 0.5 ml of Ringer’s solution was injected
intraperitoneally before injury (control), in the 2nd – solution
of choline alfoscerate at a dose 6 mg/kg (CChS activation),
in the 3rd – solution of biperidene hydrochloride at a dose
0.6 mg/kg (CChS blockade). Neurological deficits were
assessed using the 100-point Todd scale. In the blood was
determined the content of Adrenocorticotropic (ACTH)
and Thyroid-Stimulating Hormones, Corticosterone (Cs),
free Thyroxine and Triiodothyronine (fT3) by the enzyme
immunoassay. It was established that in the acute period
of TBI, post-traumatic stress central hypercortisolism is
formed with an increase in blood levels of ACTH and Cs,
and central hypothyroidism with a predominant decrease
in blood fT3. The CChS activation significantly reduced
mortality and neurological deficit, which was accompanied
by moderate activation of ACTH and Cs and no effect on the
thyroid system. The CChS blockade led to the suppression
of the post-traumatic reaction of ACTH and Cs activation
and the development of deep central hypothyroidism against
the significant neurological deficit. Thus, the important role
of CChS in the implementation of the post-traumatic stress
reaction of the neuroendocrine system has been established,
and the possibility of using pharmacological stimulation of
the CNS with central cholinomimetics has been substantiated.
Keywords:
central cholinergic systems, experimental traumatic brain injury
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