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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2020; 66(5): 46-54


AGE RELATED FEATURES OF THE BOMBESIN EFFECT ON HEPATIC BILE FORMATION

O.F. Moroz1, S.P. Veselsky2, T.P. Lyaschenko1

  1. ESC “Institute of Biology and Medicine” of Taras Shevchenko National University of Kyiv, Ukraine
  2. Institute of High technologies of Taras Shevchenko National University of Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz66.05.046

Abstract

Hepatic bile formation under bombesin action (1 µg/100 g body weight, intraportally) was examined in acute experiments on 30 male rats of three age categories: juvenile (body weight 130-175 g), adult (200-250 g) and elder (more than 300 g). Changes in the bile flow rate were recorded and the amount of various bile acids (BA) was detected using thin layer chromatography/densitometry. Bombesin caused the increase of the bile flow rate in juvenile and adult rats without any effect on it in old animals. The concentration of free bile acids increased in juvenile and adult rats and decreased in old animals. This peptide stimulated secretion of taurocholic and glicocholic acids in adult and aged rats but had no effect on the secretion of tauroconjugated dihydroxycholates. Secretion of dihydroxy bile salts conjugated with glycine was more potently enhanced by bombesin in adult rats but in the elder group this parameter dropped below the control. Bombesin had a lower effect on the BA secretion in aged rats but its influence on the juvenile animals was bidirectional. In this group the peptide stimulates free bile salts formation and, without affecting de novo synthesis, inhibits BA conjugation with amino acids in aged group. Bombesin activated classic pathway of BA biosynthesis in juvenile rats and suppressed it in aged animals. The data obtained demonstrate that different effect of bombesin on bile acids content in rats of different age could change biliary lipid-solubilizing capacity under age related damage of the gut with impairment of normal bombesin release.

Keywords: bile acids; bile flow; bombesin; conjugation; glycine; taurine; age

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