AGE RELATED FEATURES OF THE BOMBESIN EFFECT ON HEPATIC BILE FORMATION
O.F. Moroz1, S.P. Veselsky2, T.P. Lyaschenko1
- ESC “Institute of Biology and Medicine” of Taras Shevchenko National University of Kyiv, Ukraine
- Institute of High technologies of Taras Shevchenko National University of Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz66.05.046

Abstract
Hepatic bile formation under bombesin action (1 µg/100 g
body weight, intraportally) was examined in acute experiments on 30 male rats of three age categories: juvenile (body
weight 130-175 g), adult (200-250 g) and elder (more than
300 g). Changes in the bile flow rate were recorded and the
amount of various bile acids (BA) was detected using thin
layer chromatography/densitometry. Bombesin caused the
increase of the bile flow rate in juvenile and adult rats without
any effect on it in old animals. The concentration of free bile
acids increased in juvenile and adult rats and decreased in old
animals. This peptide stimulated secretion of taurocholic and
glicocholic acids in adult and aged rats but had no effect on the
secretion of tauroconjugated dihydroxycholates. Secretion of
dihydroxy bile salts conjugated with glycine was more potently
enhanced by bombesin in adult rats but in the elder group this
parameter dropped below the control. Bombesin had a lower
effect on the BA secretion in aged rats but its influence on the
juvenile animals was bidirectional. In this group the peptide
stimulates free bile salts formation and, without affecting de
novo synthesis, inhibits BA conjugation with amino acids in
aged group. Bombesin activated classic pathway of BA biosynthesis in juvenile rats and suppressed it in aged animals. The
data obtained demonstrate that different effect of bombesin on
bile acids content in rats of different age could change biliary
lipid-solubilizing capacity under age related damage of the gut
with impairment of normal bombesin release.
Keywords:
bile acids; bile flow; bombesin; conjugation; glycine; taurine; age
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